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I-Tapso Sodium CAS: 105140-25-8 Intengo Yomkhiqizi
i-3-[N-Tris(hydroxymethyl)methylamino]-2-hydroxypropanesulfonic acid,KuyimiI-sa zwitterionic buffering agent ngesimo sikasawoti we-sodium. Ivame ukusetshenziswa ekuhlolweni kwe-biochemical kanye ne-molecular biology ukuze kugcinwe i-pH ezinzile ezixazululweni.It incibilika kakhulu emanzini futhi ine-pKa eseduze ne-pH yemvelo, okwenza ifaneleke ezinhlotsheni eziningi zezicelo ezifana nokuhlanzwa kwamaprotheni, ukuhlolwa kwama-enzyme, ukukhuliswa kwamaseli, kanye ne-electrophoresis. Isakhiwo sayo samakhemikhali esiyingqayizivele kanye nokuhambisana kwayo nezinhlelo zezinto eziphilayo kuyenza ibe yingxenye ebalulekile kumaphrothokholi ahlukahlukene elebhu.
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Usawoti we-4-Nitrophenyl phosphate di(tris) CAS:68189-42-4
Usawoti i-4-Nitrophenyl phosphate di(tris) usawoti uyinhlanganisela yamakhemikhali esetshenziswa ocwaningweni lwe-biochemical kanye nokuxilongwa. Uvame ukusetshenziswa njengesisekelo sokuhlolwa kwe-enzyme ye-alkaline phosphatase. Losawoti, uma ufakwa i-hydrolyzed yi-alkaline phosphatase, ukhiqiza umkhiqizo ophuzi ongalinganiswa kalula nge-spectrophotometric. Lokhu kusabela kusetshenziswa kabanzi ezinhlotsheni ezahlukene zokusebenza, kufaka phakathi ukuhlolwa kwe-immunosorbent okuxhunywe yi-enzyme (ELISAs) kanye nokutholwa komsebenzi we-phosphatase. Ukuqina kombala ophuzi kuhambisana ngqo nomsebenzi we-enzyme, okuvumela ukulinganiswa okunembile kwamazinga e-alkaline phosphatase kumasampula ebhayoloji. Usawoti i-4-Nitrophenyl phosphate di(tris) uzinzile, uyancibilika emanzini, futhi uzwela kakhulu, okwenza kube ukukhetha okuthandwayo kokuhlolwa kwe-enzyme ocwaningweni lwebhayoloji nolwezokwelapha.
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I-Nitrotetrazolium blue chloride CAS:298-83-9
I-Nitrotetrazolium blue chloride, eyaziwa nangokuthi i-NBT noma i-NBT-Cl, iyinhlanganisela yamakhemikhali evame ukusetshenziswa ekuhlolweni kwezinto eziphilayo kanye nezamakhemikhali. Iyimpuphu ephuzi ephuzi encibilikayo emanzini.
I-NBT ivame ukusetshenziswa njengesibonakaliso sokubona ukuba khona kwama-superoxide radicals noma ama-enzyme awakhiqizayo. Ishintsha umbala uma incishiswa ama-superoxide radicals, ishintsha isuka kophuzi iye ku-deep blue formazan precipitate. Lokhu kusabela kuvame ukusetshenziswa ezivivinyweni zokufunda ukucindezeleka kwe-oxidative, ukuphefumula kwamaseli, kanye nomsebenzi wama-enzyme.
I-NBT ivame ukusetshenziswa ezindleleni ezahlukene, njengokunciphisa i-nitroblue tetrazolium, ukubona ngeso lengqondo ukukhiqizwa kwe-superoxide emaseli nasezicutshini. Iphinde yasetshenziswa ku-immunohistochemistry kanye ne-histochemistry ukuthola nokuthola amanye ama-enzyme kanye nezingxenye zamaseli.
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I-NSP-AS CAS:211106-69-3 Intengo Yomkhiqizi
I-compound 3-[9-(((3-(carboxypropyl)[4-Methxylphenyl]sulfonyl)aMine)carboxyl]-10-acridiniuMyl)-1-propanesulfonate usawoti wangaphakathi iyi-molecule eyinkimbinkimbi equkethe ingxenye ye-acridinium enamathele kusawoti wangaphakathi we-propanesulfonate. Iqukethe iqembu le-carboxypropyl, iqembu le-4-Methxylphenylsulfonyl, kanye neqembu le-amine. Le compound cishe yenzelwe ukusetshenziswa okuthile ezindaweni ezifana ne-chemistry, imithi, noma ucwaningo.
Ngaphezu kwalokho, i-NAC ibonise isithembiso ekusekeleni impilo yesibindi ngokusiza ekususeni izinto ezinobuthi, njenge-acetaminophen, umuthi ovamile wokuqeda ubuhlungu. Kungase futhi kube nemiphumela yokuvikela ekulimaleni kwesibindi okubangelwa ukuphuza utshwala.
Ngaphezu kwezakhiwo zayo zokuvikela ama-antioxidant kanye nokusekela ukuphefumula, i-NAC iye yahlolwa ngezinzuzo zayo ezingaba khona empilweni yengqondo. Ucwaningo oluthile lubonisa ukuthi ingaba nomthelela omuhle ezinkingeni zemizwa, njengokucindezeleka kanye nesifo sokuzikhukhumeza (OCD).
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I-L-Leucine p-nitroanilide hydrochloride CAS:16010-98-3
I-L-Leucine p-nitroanilide hydrochloride iyinhlanganisela yamakhemikhali eyingxenye yeqembu lezinto eziphuma ku-p-nitroanilide. Iyimpuphu yekristalu emhlophe kuya kwemhlophe evame ukusetshenziswa ocwaningweni lwe-biochemical kanye nokuhlolwa kwama-enzyme.
I-L-Leucine p-nitroanilide hydrochloride ivame ukusetshenziswa njenge-substrate ukukala umsebenzi wama-enzyme afana nama-protease nama-peptidases. Lapho la ma-enzyme ehlukana i-L-Leucine p-nitroanilide hydrochloride, ikhipha i-p-nitroaniline, engatholakala nge-spectrophotometric. Izinga lokushintsha kombala lilingana ngqo nomsebenzi we-enzyme, okuvumela ukubalwa komsebenzi we-enzyme kumasampula ebhayoloji.
Le nhlanganisela iyancibilika emanzini futhi ingancibilika kalula ezixazululweni ezifanele ze-buffer ukuze isetshenziswe ekuhlolweni kwe-enzyme. Kubalulekile ukugcina i-L-Leucine p-nitroanilide hydrochloride endaweni epholile neyomile ukuze ihlale izinzile.
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I-Guanidine Thiocyanate Sulfate CAS:593-84-0
I-Guanidine thiocyanate sulfate iyinhlanganisela yamakhemikhali etholakala kuma-ion e-guanidine, thiocyanate, kanye ne-sulfate. Ivame ukusetshenziswa ocwaningweni lwe-biology yamangqamuzana kanye ne-biochemistry njenge-ejenti ye-chaotropic.
I-Guanidine thiocyanate sulfate ivame ukusetshenziswa kumaphrothokholi okuhlukaniswa kwe-DNA ne-RNA ukuphazamisa ulwelwesi lwamaseli kanye namaprotheni aqhekekile, okuvumela ukukhishwa kahle kwama-nucleic acid. Kuyasiza ekuncibilikisweni kwe-DNA ne-RNA, okuvumela ukuhlukaniswa kwazo nokuhlanzwa kwazo kumasampula ahlukahlukene ebhayoloji.
Ngenxa yezakhiwo zayo ze-chaotropic, i-guanidine thiocyanate sulfate isiza ekuphuleni i-hydrogen bonding kanye nokunye ukusebenzisana okungena-covalent, okwenza kube lula ukuhlukaniswa kwama-nucleic acid kumaprotheni, ama-lipid, nezinye izingxenye zamaseli. Iphinde isize ekuvimbeleni umsebenzi we-RNase, iqinisekise ubuqotho nobumsulwa bama-nucleic acid akhishwe..
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I-BCIP-Toluidine)5-Bromo-4-chloro-3-indolylphosphate-p-toluidine usawoti i-CAS:6578-06-9
I-BCIP-Toluidine, eyaziwa nangokuthi usawoti we-5-Bromo-4-chloro-3-indolylphosphate-p-toluidine, iyinhlanganisela yamakhemikhali evame ukusetshenziswa ocwaningweni lwebhayoloji yama-molecule kanye ne-biochemistry. Isetshenziswa ngokuyinhloko njengesisekelo ekuhlolweni kwe-immunosorbent okuxhunywe yi-enzyme (i-ELISAs) noma amasu okufaka imibala e-immunohistochemical ukuthola umsebenzi we-alkaline phosphatase.
Uma i-BCIP-Toluidine ihlukaniswa yi-alkaline phosphatase, ibhekana nokusabela kokushintsha kombala, okukhiqiza ukuchacha okuluhlaza okwesibhakabhaka. Lokhu kusabela kuvumela ukubonakala kanye nokulinganisa umsebenzi we-alkaline phosphatase kumasampula. Ukuqina kombala kungalinganiswa nge-spectrophotometric futhi kuhambelana ngqo nomsebenzi we-enzyme.
Ukusetshenziswa kwe-BCIP-Toluidine njenge-substrate kuvame kakhulu ekusetshenzisweni okubandakanya ukulebula kwe-DNA noma i-RNA, njenge-in situ hybridization (ISH) noma i-Southern and Northern blotting. Kuhlinzeka ngendlela ethembekile nebucayi yokuthola ukuba khona kwama-nucleic acid aqondiwe kumasampula.
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I-3-Aminophthalhydrazide CAS:521-31-3
I-3-Aminophthalhydrazide iyinhlanganisela yamakhemikhali enefomula yama-molecule i-C8H8N2O. Ibizwa ngokuthi i-aminohydrazide derivative ye-phthalic acid. Le nhlanganisela yakhiwe umgogodla we-phthalic acid onama-athomu amabili e-hydrogen athathelwe indawo yiqembu lama-amino kanye neqembu le-hydrazine.
I-3-Aminophthalhydrazide inezinhlelo ezahlukene zokusetshenziswa emkhakheni we-organic chemistry kanye nemithi. Ingasetshenziswa njengento yokuqala ekuhlanganisweni kwamakhemikhali ahlukahlukene emithi kanye nodayi. Ngaphezu kwalokho, ivame ukusetshenziswa ekulungiseleleni izisekelo ze-Schiff, okuyizinto ezibalulekile ekuhlanganisweni kwezinto eziphilayo.
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4-Aminoantipyrine CAS: 83-07-8
I-4-Aminoantipyrine, eyaziwa nangokuthi i-aminoantipyrine noma i-aminopyrine, iyinhlanganisela yezinto eziphilayo ezenziwe ngokwenziwa. Ivame ukusetshenziswa njenge-reagent embonini yamakhemikhali okuhlaziya kanye nemithi. Isakhiwo sayo samakhemikhali siqukethe indandatho enuka kamnandi eneqembu lama-amino (NH2) kanye neqembu le-pyrazolone, okwenza kube yinkimbinkimbi eguquguqukayo yezinhlelo zokusebenza ezahlukahlukene.
Kumakhemikhali okuhlaziya, i-4-Aminoantipyrine isetshenziswa kabanzi njenge-reagent ye-chromogenic ekuhlolweni kwe-colorimetric. Isabela nezinto ezithile, njenge-phenols nama-peroxide, ukuze yakhe ama-complexes anemibala angalinganiswa nge-spectrophotometric. Lesi sici siyenza ibe usizo ekuhlolweni okuhlukahlukene, okuhlanganisa nokunqunywa kwama-enzyme afana ne-peroxidase, i-glucose, ne-amylase.
Embonini yezemithi, i-4-Aminoantipyrine ibilokhu isetshenziswa njengomuthi wokudambisa ubuhlungu kanye nowokulwa nokuvuvukala. .
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I-TAPS-NA CAS:91000-53-2 Intengo Yomkhiqizi
I-N-[Tris(hydroxymethyl)methyl]-3-aminopropanesulfonic acid usawoti we-sodium, owaziwa nangokuthi i-TAPS-Na, iyinhlanganisela yamakhemikhali evame ukusetshenziswa njenge-ejenti yokubopha ezinhlotsheni ezahlukahlukene zebhayoloji kanye ne-biochemical. Iyinhlanganisela ye-zwitterionic, okusho ukuthi inamashaji amahle nangalungile, okusiza ukugcina i-pH ezinzile ezixazululweni.
I-TAPS-Na inobubanzi be-pH obungaba ngu-7.7 kuya ku-9.1, okwenza ifaneleke ukusetshenziswa okudinga indawo ye-pH ene-alkaline kancane. Ivame ukusetshenziswa ocwaningweni lwamaprotheni nama-enzyme, kanye nasemasu e-molecular biology njenge-gel electrophoresis kanye ne-DNA sequencing.
Njengosawoti we-sodium, i-TAPS-Na incibilika kakhulu emanzini, okuvumela ukulungiswa okulula kwezixazululo ezihlanganisiwe. Iyaziwa ngobuthi bayo obuphansi kanye nokuphazamiseka okuncane kwama-molecule ezinto eziphilayo, okwenza kube ukukhetha okuthandwayo kokuhlolwa okuningi.
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5,5-Dithiobis (2-nitrobenzoic acid) CAS:69-78-3
I-5,5-Dithiobis (2-nitrobenzoic acid), eyaziwa nangokuthi i-DTNB noma i-Ellman's reagent, iyinhlanganisela yamakhemikhali evame ukusetshenziswa ocwaningweni lwe-biochemical kanye nemithi. Iyimpuphu ephuzi, ekristalu encibilika kakhulu emanzini nasezincibilikisini eziphilayo.
I-DTNB isetshenziswa kakhulu ukukala ukuhlushwa kwamaqembu e-sulfhydryl (-SH) kumaprotheni nama-peptide. Inhlanganisela isabela nama-thiol, okuholela ekwakhekeni kwe-disulfide exubile bese ikhipha i-2-nitro-5-thiobenzoate, enombala ophuzi ongalinganiswa nge-spectrophotometric. Ngokulinganisa ubukhulu bombala ophuzi, kungenzeka ukulinganisa ukuhlushwa kwama-thiol kusampula. Le ndlela ivame ukusetshenziswa ukutadisha ukusebenzisana kwamaprotheni nama-protein, ukugoqwa kwamaprotheni, kanye nokusabela kwama-enzyme okubandakanya amaqembu e-thiol.
Ngaphezu kokulinganisa kwe-thiol, i-DTNB isetshenziswe kwezinye izinhlelo zokusebenza, njengokuhlola isimo se-redox samaseli, ukutadisha ukuzinza kwamakhemikhali e-metal-sulfur, kanye nokulinganisa umsebenzi wama-enzyme ahlukahlukene.
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I-PHENOLPHTHALEIN MONOPHOSPHATE BIS(CYCLOHEXYLAMMONIUM) USAWOTI I-CAS:14815-59-9
I-Phenolphthalein monophosphate I-dicyclohexylammonium salt iyinhlanganisela yamakhemikhali etholakala ku-phenolphthalein, inkomba ye-pH evame ukusetshenziswa ezindaweni zelebhu. Uhlobo lwe-dicyclohexylammonium salt lwe-phenolphthalein monophosphate luvame ukusetshenziswa njenge-reagent yokuhlaziya ocwaningweni lwe-biochemical kanye ne-molecular biology. Isetshenziswa kakhulu ekuhlolweni noma ekuhlolweni ukuthola nokukala ubukhona bama-enzyme athile, kufaka phakathi lawo ahilelekile emisebenzini ye-ATP (adenosine triphosphate) hydrolysis noma i-phosphatase. Izakhiwo zayo ezihlukile ziyenza ithuluzi eliwusizo ocwaningweni lwesayensi oluhlobene ne-enzyme kinetics kanye nezinqubo zamaseli..
