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I-Ninhydrin hydrate CAS: 485-47-2 Intengo Yomkhiqizi
I-Ninhydrin hydrate iyinhlanganisela yamakhemikhali evame ukusetshenziswa ekuhlaziyweni kwe-forensic kanye ne-organic chemistry. Iyimpuphu yekristalu engenambala noma ephuzi ekhanyayo encibilikayo emanzini nasezincibilikisini eziphilayo.
I-Ninhydrin hydrate yaziwa ngekhono layo lokusabela nama-amino acid kanye nama-amine ayinhloko, okukhiqiza umbala obonakalayo onsomi-oluhlaza okwesibhakabhaka. Lokhu kusabela kuvame ukusetshenziswa ekuhlaziyweni kweminwe ye-forensic ukuze kubonwe futhi kuthuthukiswe ukubonakala kweminwe efihliwe ezindaweni ezinezimbobo. Isixazululo se-ninhydrin sivame ukufuthwa phezu kobuso, futhi iminwe yeminwe ikhula ngokuhamba kwesikhathi njengoba i-ninhydrin isabela nama-amino acid akhona ezinsaleleni zeminwe yeminwe.
Kumakhemikhali e-organic, i-ninhydrin hydrate isetshenziswa njenge-reagent yokuthola nokuhlaziya ama-amino acid, ama-peptide, namaprotheni. Ingasetshenziswa ukunquma ubukhona kanye nokulinganisa inani lama-amino acid kusampula. Ukusabela nge-ninhydrin kuphumela ekwakhekeni komkhiqizo onombala, ongalinganiswa nge-spectrophotometric ukuze kulinganiswe ukuhlushwa kwe-analyte.
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I-D-Luciferin CAS: 2591-17-5 Intengo Yomkhiqizi
I-D-Luciferin iyi-molecule encane ekhipha ukukhanya etholakala kakhulu ezintweni eziphilayo ezikhanyayo njenge-fireflies, amabhaktheriya, kanye nezinto eziphilayo zasolwandle. Iyingxenye ebalulekile yokusabela kwe-luciferase, inqubo yamakhemikhali ekhiqiza ukukhanya.
I-D-Luciferin ivame ukusetshenziswa njengesisekelo ekuhlolweni kwe-bioluminescence kanye namasu okuthwebula izithombe, lapho ukusabela kwayo ne-luciferase kukhiqiza ukukhanya okungatholakala futhi kulinganiswe. Lokhu kuyenza ithuluzi eliwusizo ezindaweni ezahlukene zocwaningo, okuhlanganisa i-molecular biology, ukutholakala kwezidakamizwa, kanye nokuthwebula izithombe kwezokwelapha.
Ngenxa yezakhiwo zayo ezihlukile zokubona, i-D-Luciferin isetshenziswe kabanzi ezifundweni ezihilela ukubonakaliswa kwezakhi zofuzo, ukusebenzisana kwamaprotheni namaphrotheni, kanye nokusabalalisa amaseli. Inikeza indlela engangenisi uhlaselo futhi ebucayi yokuphenya izinqubo zezinto eziphilayo ngesikhathi sangempela.
Ngaphezu kwalokho, kuye kwathuthukiswa ama-derivatives e-D-Luciferin nama-analog, okuvumela ukuguqulwa okuhlukahlukene kanye nezinhlelo zokusebenza. Lawa ma-derivatives aguquliwe avumela amaza ahlukahlukene okukhishwa kokukhanya, ukuqina okuthuthukisiwe, kanye nokungena kwezicubu okuthuthukisiwe.
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i-p-hydroxybenzoic acid, i-monosodium CAS:114-63-6
I-p-Hydroxybenzoic acid, i-monosodium (eyaziwa nangokuthi i-sodium 4-hydroxybenzoate) iyinhlanganisela yamakhemikhali enefomula yama-molecule i-C7H5NaO3. Iwusawoti we-sodium otholakala ku-p-hydroxybenzoic acid, okuyinhlanganisela yemvelo etholakala ezitshalweni ezifana nezithelo nemifino.
I-p-Hydroxybenzoic acid, i-monosodium isetshenziswa ezimbonini ezahlukahlukene ngenxa yezakhiwo zayo zokulwa namagciwane. Ivame ukusetshenziswa njengesivikelo ekudleni, eziphuzweni, ezimonyweni, nasemikhiqizweni yokunakekela umuntu siqu ukuvimbela ukukhula kwamagciwane, isikhunta, kanye nemvubelo. Isiza ukwandisa impilo yale mikhiqizo ngokuvimbela ukonakala nokugcina ikhwalithi yayo.
Ekusetshenzisweni kwemithi, i-p-Hydroxybenzoic acid, i-monosodium isetshenziswa njengesithako emithini yomlomo kanye nasemithini yokwelapha. Izakhiwo zayo zokulwa namagciwane zisiza ekugcineni ukuzinza kanye nokungazali kwale mikhiqizo, ukuqinisekisa ukuphepha nokusebenza kwayo.
Ngaphezu kwalokho, i-p-Hydroxybenzoic acid, i-monosodium inezakhiwo ze-antioxidant, ezenza kube usizo ekuvimbeleni umonakalo we-oxidative emikhiqizweni eyahlukahlukene. Ingasiza ekuvikeleni ekuwohlokeni kwezithako futhi yandise ukuqina komkhiqizo..
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4-Nitrophenyl phosphate disodium salt hexahydrate CAS:4264-83-9
I-4-Nitrophenyl phosphate disodium salt hexahydrate iyinhlanganisela yamakhemikhali evame ukusetshenziswa njengesisekelo sokuthola umsebenzi wama-enzyme e-phosphatase. Ibonakala njengempuphu emhlophe kuya kwemhlophe futhi incibilika kakhulu emanzini. Uma isetshenziswa ama-enzyme e-phosphatase, ibhekana nokusabela okuholela ekukhiqizweni kombala ophuzi, ongalinganiswa nge-spectrophotometric. Le nhlanganisela ithola ukusetshenziswa ezivivinyweni ezahlukahlukene ze-biochemical kanye nama-diagnostic kit okuthola nokulinganisa umsebenzi we-phosphatase kumasampula..
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I-Methylphenazinium methosulfate CAS:299-11-6
I-Methylphenazinium methosulfate (MPMS) iyinhlanganisela esebenza nge-redox evame ukusetshenziswa njengesithwali sama-electron ezifundweni ezahlukahlukene ze-biochemical kanye ne-biophysical. Iwusawoti oqukethe i-methylphenazinium cation (inhlanganisela ye-heterocyclic) kanye ne-methosulfate anion.
I-MPMS ivame ukusetshenziswa njengendlela ehlukile kunezinye izithwali zama-electron zendabuko, njenge-ferricyanide noma i-phenazine ethosulfate, ngenxa yokuqina kwayo kanye nokuncibilika kwayo okuphezulu emanzini. Inezakhiwo ezinhle ze-redox, ezivumela ukuthi yamukele futhi idlulise ama-electron ngesikhathi sokuphendula kwe-enzyme.
Enye yezindlela ezibalulekile zokusebenzisa i-MPMS kusezihlolweni ezihilela ukukalwa kokudluliswa kwama-electron noma umsebenzi we-enzyme. Ivame ukusetshenziswa kanye nohlelo lwe-enzyme ukuqapha ukudluliswa kwama-electron phakathi kwezingxenye ezahlukene. Ukuncipha kwe-MPMS kungatholakala nge-spectrophotometric, lapho ukumuncwa kwayo kushintsha khona ngenxa yezinqubo zokudluliswa kwama-electron.
I-MPMS isetshenziswa nasezifundweni ezihlobene nokuphefumula kwe-mitochondrial kanye ne-oxidative phosphorylation. Ingasebenza njengokwamukela ama-electron okwenziwa, okuvumela abacwaningi ukuthi baphenye ukusebenza kanye nokulawulwa kwalezi zinqubo ezinhlelweni ezahlukene zezinto eziphilayo.
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4-Nitrophenyl-alpha-L-fucopyranoside CAS:10231-84-2
I-4-Nitrophenyl-alpha-L-fucopyranoside iyinhlanganisela yamakhemikhali eyingxenye yomndeni wama-glycoside. Yakhiwa i-molecule kashukela we-fucose exhunywe eqenjini le-4-nitrophenyl. Le nhlanganisela ivame ukusetshenziswa njenge-substrate ekuhlolweni kwe-enzyme ukuze kufundwe umsebenzi wama-fucosidases, angama-enzyme ahilelekile ekuqhekekeni kwama-molecule aqukethe i-fucose. Uma isetshenziswa yi-enzyme ye-fucosidase, i-4-nitrophenyl-alpha-L-fucopyranoside iyaqhekeka, okuholela ekukhishweni kwe-4-nitrophenol, engalinganiswa ngobuningi kusetshenziswa i-spectrophotometry. Le substrate iwusizo kakhulu ezifundweni ezihlobene nomsebenzi we-enzymatic, ukucaciswa kwe-substrate, ukuhlolwa kwe-inhibitor, kanye ne-kinetics yama-enzyme e-fucosidase.
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I-N-Ethylmaleimide CAS:128-53-0 Intengo Yomkhiqizi
I-N-Ethylmaleimide (NEM) iyinhlanganisela encane ephilayo evame ukusetshenziswa ocwaningweni lwe-biochemistry kanye ne-molecular biology. Isebenza njengesivimbeli esithile samaqembu e-protein sulfhydryl (thiol) ngokushintsha nokuvimba ngokungenakuguqulwa umsebenzi wawo. I-NEM isabela kakhulu kumaqembu e-sulfhydryl, njengalawo atholakala ku-amino acid cysteine, futhi ingasabela kokubili namaqembu e-sulfhydryl amahhala kanye nalawo angaphakathi kwamaprotheni. Lokhu kwenza i-NEM ibe ithuluzi eliwusizo lokufunda umsebenzi wamaprotheni, ukusebenzisana kwamaprotheni namaprotheni, kanye nomsebenzi we-enzyme. Izici zayo zokuvimbela zisetshenziswe ezinhlobonhlobo zezicelo, kufaka phakathi i-proteomics, i-enzymology, i-structural biology, kanye nokutholakala kwezidakamizwa.
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I-5-Sulfosalicylic acid dihydrate CAS:5965-83-3
I-5-Sulfosalicylic acid dihydrate iyinhlanganisela yamakhemikhali evame ukusetshenziswa emalabhorethri e-biochemical kanye nemithi. Iyinhlobo emhlophe yekristalu encibilika kakhulu emanzini. I-5-Sulfosalicylic acid dihydrate iyinhlobo ye-salicylic acid futhi inefomula yama-molecule ye-C7H6O6S. Ivame ukusetshenziswa njenge-reagent yokukhipha amaprotheni futhi ingasetshenziswa ukukala amaprotheni kumasampula ebhayoloji, njengomchamo kanye ne-serum. Ngaphezu kwalokho, ingasetshenziswa ekunqumeni izinto ezahlukahlukene, kufaka phakathi ama-nucleic acid, ama-enzyme, ama-hormone, kanye nemithi. Uhlobo lwe-dihydrate lwenhlanganisela lubonisa ukuthi iqukethe ama-molecule amabili amanzi nge-molecule ngayinye ye-asidi.
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I-potassium iodide CAS:7681-11-0
I-Potassium iodide (KI) iyinhlanganisela engeyona into ephilayo equkethe ama-potassium cations (K+) kanye nama-iodide anions (I-). Iyisithako esimhlophe, esicwebezelayo esincibilika kakhulu emanzini. I-Potassium iodide inezindlela ezahlukene zokusetshenziswa ngenxa yezakhiwo zayo.
Enye yezindlela eziyinhloko zokusebenzisa i-potassium iodide isemithini. Ivame ukusetshenziswa njengesengezo sokwelapha nokuvimbela izimo ezihlobene nokuntuleka kwe-iodine, njenge-goiter, ukungalingani kwama-hormone e-thyroid, kanye nezinhlobo ezithile zomdlavuza we-thyroid. Ingasetshenziswa futhi njengokwelashwa okuphuthumayo kokuchayeka emisebeni, njengoba kusiza ukuvimba ukumuncwa kwe-iodine emisebeni yi-thyroid gland.
I-potassium iodide nayo inokusetshenziswa ezindaweni zelebhu. Isetshenziswa njenge-reagent kumakhemikhali okuhlaziya ukuthola ukuba khona kwezinto ezithile, njenge-lead kanye ne-mercury, ngokwakhiwa kwama-precipitates aphuzi angancibiliki. Ngaphezu kwalokho, ingasetshenziswa njengomthombo wama-ion e-iodide ekusabeleni okuhlukahlukene kwamakhemikhali.
Emkhakheni wokupheka, i-potassium iodide ngezinye izikhathi ifakwa kusawoti wetafula (usawoti one-iodized) njengendlela yokuvimbela ukuntuleka kwe-iodine kubantu abaningi. Usawoti one-iodized udliwa abantu abaningi emhlabeni jikelele njengomthombo wokudla we-iodine.
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8-Anilino-1-naphthalenesulfonic acid usawoti we-ammonium CAS:28836-03-5
I-8-Anilino-1-naphthalenesulfonic acid Usawoti we-ammonium uyinhlanganisela yamakhemikhali evame ukusetshenziswa njengodayi we-fluorescent. Inesakhiwo esakhiwe yiqembu le-aniline elixhunywe ku-molecule ye-naphthalenesulfonic acid enosawoti we-ammonium. Le nhlanganisela iyancibilika emanzini futhi ikhombisa ukumuncwa okunamandla nokukhishwa kwayo ebangeni lokukhanya okubonakalayo.
Ngenxa yezakhiwo zayo ezikhanyayo, usawoti we-ammonium we-8-Anilino-1-naphthalenesulfonic acid uvame ukusetshenziswa njenge-probe noma inkomba ezifundweni ezahlukahlukene ze-biochemical kanye ne-biophysical. Ungasetshenziswa ukuthola izinguquko ku-pH, izinguquko zesakhiwo kumaprotheni, ukusebenzisana kwamaprotheni ne-ligand, kanye nokuba khona kwama-ion athile.
Uma ivuselelwa ubude bokukhanya obufanele, le nhlanganisela ikhipha ukukhanya okuluhlaza okwesibhakabhaka okukhanyayo, okuvumela ukutholakala nokulinganiswa okulula. Izakhiwo zayo zokukhanya ziyenza ithuluzi eliwusizo ezindaweni zocwaningo ezifana ne-molecular biology, i-biochemistry, kanye ne-cell biology.
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I-Sodium dodecyl sulfate CAS:151-21-3
I-Sodium dodecyl sulfate (SDS) iyi-anionic surfactant esetshenziswa kakhulu esetshenziswa ezimbonini ezahlukahlukene kanye nezicelo zocwaningo. Iyi-solid noma i-powder emhlophe encibilikayo emanzini futhi yakha i-foam eqinile, eqinile uma ishukunyiswa. I-SDS yaziwa ngekhono layo lokuncibilikisa nokuguqula amaprotheni, okwenza kube usizo ekukhipheni amaprotheni, ekuhlanzeni, kanye nasemasu e-electrophoresis. Ngaphezu kwalokho, i-SDS isetshenziswa kabanzi njengesihlanzi emikhiqizweni yokunakekelwa kwasekhaya kanye neyomuntu siqu, kanye nasezinhlelweni zokuhlanza izimboni. Futhi iyisithako esibalulekile ezivivinyweni eziningi ze-biochemical kanye ne-molecular biology, lapho isetshenziselwa khona i-cell lysis, i-DNA isolation, kanye ne-ejenti yokunciphisa ubukhona ku-gel electrophoresis.
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I-BCA-2K CAS:207124-63-8 Intengo Yomkhiqizi
I-BCA-2K imele i-Beta-Carotene Apocarotenoid-2-Ketolase. Iyi-enzyme edlala indima ebalulekile ekwakhiweni kwemibala yezitshalo ebizwa ngokuthi ama-apocarotenoids. Lawa mabala atholakala ku-molecule eyandulela i-beta-carotene futhi ahilelekile ezinqubweni ezahlukene zezinto eziphilayo ezitshalweni.
I-BCA-2K ikhuthaza ngqo ukuguqulwa kwe-beta-carotene ibe ama-apocarotenoid athile ngokufaka iqembu le-ketone ku-molecule. Lokhu kusabela kwe-enzyme kubalulekile ekukhiqizweni kwama-apocarotenoid athile anemisebenzi ehlukene yezinto eziphilayo kanye nemisebenzi ezitshalweni.
Ama-apocarotenoid akhiqizwa yi-BCA-2K atholakale edlala indima ekuthuthukisweni kwezitshalo, ekuphenduleni ukucindezeleka, nasekuvikeleni ezifweni ezibangelwa amagciwane kanye nezinambuzane. Angasebenza futhi njengezimpawu zama-molecule ahilelekile ezinqubweni ezahlukene zomzimba.
Ucwaningo lwe-BCA-2K kanye nendima yalo ekwakhiweni kwe-apocarotenoid alubalulekile nje kuphela ekuqondeni i-biology yezitshalo kodwa futhi lunezinhlelo zokusebenza ezingaba khona ezindaweni ezifana nokuthuthukiswa kwezitshalo, ukufuya izitshalo, kanye nokuthuthukiswa kwemikhiqizo yemvelo enezinzuzo zokwelapha noma zokudla okunempilo.
