I-BCIP-Toluidine)5-Bromo-4-chloro-3-indolylphosphate-p-toluidine usawoti i-CAS:6578-06-9

I-BCIP-Toluidine, eyaziwa nangokuthi usawoti we-5-Bromo-4-chloro-3-indolylphosphate-p-toluidine, iyinhlanganisela yamakhemikhali evame ukusetshenziswa ocwaningweni lwebhayoloji yama-molecule kanye ne-biochemistry. Isetshenziswa ngokuyinhloko njengesisekelo ekuhlolweni kwe-immunosorbent okuxhunywe yi-enzyme (i-ELISAs) noma amasu okufaka imibala e-immunohistochemical ukuthola umsebenzi we-alkaline phosphatase.

Uma i-BCIP-Toluidine ihlukaniswa yi-alkaline phosphatase, ibhekana nokusabela kokushintsha kombala, okukhiqiza ukuchacha okuluhlaza okwesibhakabhaka. Lokhu kusabela kuvumela ukubonakala kanye nokulinganisa umsebenzi we-alkaline phosphatase kumasampula. Ukuqina kombala kungalinganiswa nge-spectrophotometric futhi kuhambelana ngqo nomsebenzi we-enzyme.

Ukusetshenziswa kwe-BCIP-Toluidine njenge-substrate kuvame kakhulu ekusetshenzisweni okubandakanya ukulebula kwe-DNA noma i-RNA, njenge-in situ hybridization (ISH) noma i-Southern and Northern blotting. Kuhlinzeka ngendlela ethembekile nebucayi yokuthola ukuba khona kwama-nucleic acid aqondiwe kumasampula.

Oda noma Cela Isilinganiso

Imininingwane Yomkhiqizo

Amathegi Omkhiqizo

Ukusetshenziswa kanye Nomphumela

I-BCIP-Toluidine (usawoti we-5-Bromo-4-chloro-3-indolylphosphate-p-toluidine) iyi-substrate esetshenziswa kumasu e-molecular biology ukuthola ukuthi kukhona umsebenzi we-alkaline phosphatase. Uma isetshenziswa njenge-substrate, i-BCIP-Toluidine ibhekana nokusabela kokushintsha kombala lapho iqhekeka yi-alkaline phosphatase, okuholela ekwakhekeni kwe-precipitate eluhlaza okwesibhakabhaka. Lokhu kushintsha kombala kuvumela abacwaningi ukuthi babone futhi balinganise umsebenzi we-alkaline phosphatase ezinhlotsheni ezahlukene zokuhlolwa.

Ukusetshenziswa okuyinhloko kwe-BCIP-Toluidine kusezinhlolweni ze-immunosorbent ezixhunywe yi-enzyme (i-ELISA) kanye namasu okupenda nge-immunohistochemical. Ivame ukusetshenziselwa ukuthola ama-antibodies ahlanganiswe yi-alkaline phosphatase noma ama-probe anelebula le-alkaline phosphatase kulezi zivivinyo. I-BCIP-Toluidine ivame ukusetshenziswa ocwaningweni lwe-molecular biology ukuze ibone futhi ilinganise ukubonakaliswa kwezakhi zofuzo, indawo yamaprotheni, kanye nomsebenzi we-enzyme.

Ukupakisha Umkhiqizo:

Ulwazi Olwengeziwe:

Ukwakheka	C8H6BrClNO4P.C7H9N
Ukuhlolwa	99%
Ukubukeka	Impuphu emhlophe
Inombolo ye-CAS	6578-06-9
Ukupakisha	Encane futhi enkulu
Impilo Yeshelufu	iminyaka engu-2
Isitoreji	Gcina endaweni epholile neyomile
Isitifiketi	I-ISO.