Usawoti we-4-Nitrophenyl phosphate di(tris) CAS:68189-42-4

Usawoti we-4-Nitrophenyl phosphate di(tris) uvame ukusetshenziswa njengesisekelo se-alkaline phosphatase ekuhlolweni okuhlukahlukene kwe-biochemical kanye ne-enzyme. Uma i-hydrolyzed yi-alkaline phosphatase, ikhiqiza umkhiqizo ophuzi ongalinganiswa kalula nge-spectrophotometric. Lokhu kusabela kusetshenziswa kabanzi ezicelweni ezifana ne-enzyme-linked immunosorbent assays (ELISAs) kanye nokutholwa komsebenzi we-phosphatase.

Ukuqina kombala ophuzi okukhiqizwayo kuhambisana ngqo nomsebenzi we-enzyme, okuvumela ukulinganiswa okunembile kwamazinga e-alkaline phosphatase kumasampula ezinto eziphilayo. Lokhu kuyenza ibe usizo ezindaweni zezokwelapha nezocwaningo zokufunda umsebenzi we-alkaline phosphatase kumaseli, izicubu, kanye noketshezi lwezinto eziphilayo.

Ngaphezu kwalokho, usawoti we-4-Nitrophenyl phosphate di(tris) uzinzile futhi uncibilika kakhulu emanzini, okwenza ulungele izimo ezahlukahlukene zokuhlola. Ukuzwela kwawo okuphezulu kanye nokulula kokulinganisa kwenza kube ukukhetha okuthandwayo njengesisekelo sokuhlolwa kwama-enzyme, okusiza ekuhlolweni kwe-enzyme kinetics, umsebenzi wama-enzyme, kanye nokutholakala kwezidakamizwa.