5,5-Dithiobis (2-nitrobenzoic acid) CAS:69-78-3

I-5,5-Dithiobis (2-nitrobenzoic acid), eyaziwa nangokuthi i-DTNB noma i-Ellman's reagent, iyinhlanganisela yamakhemikhali evame ukusetshenziswa ocwaningweni lwe-biochemical kanye nemithi. Iyimpuphu ephuzi, ekristalu encibilika kakhulu emanzini nasezincibilikisini eziphilayo.

I-DTNB isetshenziswa kakhulu ukukala ukuhlushwa kwamaqembu e-sulfhydryl (-SH) kumaprotheni nama-peptide. Inhlanganisela isabela nama-thiol, okuholela ekwakhekeni kwe-disulfide exubile bese ikhipha i-2-nitro-5-thiobenzoate, enombala ophuzi ongalinganiswa nge-spectrophotometric. Ngokulinganisa ubukhulu bombala ophuzi, kungenzeka ukulinganisa ukuhlushwa kwama-thiol kusampula. Le ndlela ivame ukusetshenziswa ukutadisha ukusebenzisana kwamaprotheni nama-protein, ukugoqwa kwamaprotheni, kanye nokusabela kwama-enzyme okubandakanya amaqembu e-thiol.

Ngaphezu kokulinganisa kwe-thiol, i-DTNB isetshenziswe kwezinye izinhlelo zokusebenza, njengokuhlola isimo se-redox samaseli, ukutadisha ukuzinza kwamakhemikhali e-metal-sulfur, kanye nokulinganisa umsebenzi wama-enzyme ahlukahlukene.

Oda noma Cela Isilinganiso

Imininingwane Yomkhiqizo

Amathegi Omkhiqizo

Ukusetshenziswa kanye Nomphumela

Ukulinganiswa kwe-Thiol: I-DTNB ivame ukusetshenziswa ukuhlola ukuhlushwa kwama-thiol kumasampula amaprotheni. Lolu lwazi lubalulekile ekutadisheni ukusebenzisana kwamaprotheni namaphrotheni, ukugoqwa kwamaprotheni, kanye nokusabela kwama-enzyme okubandakanya amaqembu e-thiol. Impendulo ye-colorimetric ye-DTNB ivumela ukulinganiswa kwama-thiol ngokusebenzisa izilinganiso ze-spectrophotometric.

Ukuhlolwa Kwesimo Se-Redox: I-DTNB isetshenziswe ukuhlola isimo se-redox ngaphakathi kwamaseli, izicubu, noma amasampula ebhayoloji. Ngokulinganisa izinguquko ekugxilweni kwe-thiol, abacwaningi bangathola ukuqonda ngokucindezeleka kwe-oxidative, umsebenzi we-antioxidant, kanye nezindlela zokubonisana ze-redox.

Ukuhlolwa Kwe-Enzyme: Ukusebenza okuyingqayizivele kwe-DTNB ngama-thiols kuyenza ibe ithuluzi eliwusizo lokuhlolwa kwe-enzyme. Ama-enzyme amaningi, njenge-glutathione-S-transferases, i-cystathionine-γ-lyase, kanye ne-thioredoxin reductase, ancike ku-thiol futhi angalinganiswa noma ahlolwe kusetshenziswa i-DTNB.

Ukuhlaziywa Kokuzinza Kwama-Metal-Sulfur Compounds: I-DTNB isetshenziselwa ukuhlola ukuzinza kanye nokusabela kwama-metal-sulfur complexes kanye nama-metalloproteins. I-compound ingathola futhi ilinganise ubukhona bama-thiols ahilelekile ekuqhekekeni kwama-ion ensimbi, inikeze ulwazi olubalulekile mayelana nokusebenzisana kwe-metal-sulfur kanye ne-chemistry yokuxhumana.

Ukupakisha Umkhiqizo:

Ulwazi Olwengeziwe:

Ukwakheka	C14H8N2O8S2
Ukuhlolwa	99%
Ukubukeka	Okuqinile okuphuzi
Inombolo ye-CAS	69-78-3
Ukupakisha	Encane futhi enkulu
Impilo Yeshelufu	iminyaka engu-2
Isitoreji	Gcina endaweni epholile neyomile
Isitifiketi	I-ISO.