I-Nitrotetrazolium blue chloride CAS:298-83-9

I-Nitrotetrazolium blue chloride, eyaziwa nangokuthi i-NBT noma i-NBT-Cl, iyinhlanganisela yamakhemikhali evame ukusetshenziswa ekuhlolweni kwezinto eziphilayo kanye nezamakhemikhali. Iyimpuphu ephuzi ephuzi encibilikayo emanzini.

I-NBT ivame ukusetshenziswa njengesibonakaliso sokubona ukuba khona kwama-superoxide radicals noma ama-enzyme awakhiqizayo. Ishintsha umbala uma incishiswa ama-superoxide radicals, ishintsha isuka kophuzi iye ku-deep blue formazan precipitate. Lokhu kusabela kuvame ukusetshenziswa ezivivinyweni zokufunda ukucindezeleka kwe-oxidative, ukuphefumula kwamaseli, kanye nomsebenzi wama-enzyme.

I-NBT ivame ukusetshenziswa ezindleleni ezahlukene, njengokunciphisa i-nitroblue tetrazolium, ukubona ngeso lengqondo ukukhiqizwa kwe-superoxide emaseli nasezicutshini. Iphinde yasetshenziswa ku-immunohistochemistry kanye ne-histochemistry ukuthola nokuthola amanye ama-enzyme kanye nezingxenye zamaseli.

Oda noma Cela Isilinganiso

Imininingwane Yomkhiqizo

Amathegi Omkhiqizo

Ukusetshenziswa kanye Nomphumela

I-Nitrotetrazolium blue chloride (NBT) iwudayi ovame ukusetshenziswa ekuhlolweni kwezinto eziphilayo kanye nezamakhemikhali. Isetshenziswa kakhulu njengesibonakaliso se-redox ukuthola nokubona ngeso lengqondo ukuba khona kwama-enzyme e-dehydrogenase kumasampula ezinto eziphilayo.

Uma i-NBT incishiswa ama-enzyme e-dehydrogenase, ishintsha umbala kusukela kokuphuzi okukhanyayo kuya kokuluhlaza okwesibhakabhaka okumnyama. Lokhu kuguqulwa kuvumela abacwaningi ukuthi babone umsebenzi kanye nendawo yama-enzyme e-dehydrogenase ezicutshini nasemaseli ahlukahlukene. Ngenxa yalokho, i-NBT ivame ukusetshenziswa ekuhlolweni kokuxilonga kanye nasezifundweni ze-immunohistochemical ukuhlola umsebenzi we-metabolic weseli kanye nokusebenza kwama-enzyme.

Ngokulinganisa ubukhulu bombala oluhlaza okwesibhakabhaka owakhiwe, abacwaningi bangahlaziya ngobuningi umsebenzi we-enzyme ye-dehydrogenase, okubalulekile ekuqondeni izinqubo zamaseli njengokucindezeleka kwe-oxidative, ukuphefumula kwamaseli, kanye nokusetshenziswa kwama-carbohydrate nama-lipid. Lolu lwazi lubalulekile emikhakheni ehlukahlukene, kufaka phakathi i-biochemistry, i-cell biology, kanye nokuxilongwa kwemitholampilo.

Ukupakisha Umkhiqizo:

Ulwazi Olwengeziwe:

Ukwakheka	C40H30ClN10O6+
Ukuhlolwa	99%
Ukubukeka	Impuphu ephuzi
Inombolo ye-CAS	298-83-9
Ukupakisha	Encane futhi enkulu
Impilo Yeshelufu	iminyaka engu-2
Isitoreji	Gcina endaweni epholile neyomile
Isitifiketi	I-ISO.