I-Tapso Sodium CAS: 105140-25-8 Intengo Yomkhiqizi

Ukuzinzisa i-pH: I-TAPS ivame ukusetshenziswa njenge-buffer yokuzinzisa i-pH yezixazululo ekuhlolweni nasekusetshenzisweni okuhlukahlukene. Ine-pKa eseduze ne-pH yemvelo, cishe engu-8.5, okwenza ifaneleke kakhulu ekugcineni i-pH ezinzile ezinhlelweni zezinto eziphilayo.

Izifundo zamaphrotheni: I-TAPS isetshenziswa kabanzi ezifundweni ze-biochemistry yamaphrotheni kanye ne-structural biology. Ivame ukusetshenziswa njenge-ejenti yokuvimba izixazululo zamaphrotheni kanye nama-buffer ukuze kugcinwe ukwakheka kweprotheni kanye nokuqina kwayo ngesikhathi sokuhlanzwa, ukugcinwa, kanye nokuhlolwa. I-TAPS iwusizo kakhulu lapho isebenza ngamaphrotheni azwela ushintsho lwe-pH.

Ukuhlolwa kwe-enzyme: I-TAPS ivame ukusetshenziswa njenge-buffer ekuhlolweni kwe-enzyme, lapho i-pH idinga ukulawulwa ngokunembile. Amandla ayo okugcina i-buffer kanye nokuqina kwayo kuyenza ibe yisinqumo esithandwayo sezinhlobo eziningi ze-enzyme reactions.

Ukukhuliswa kwamaseli: I-TAPS ivame ukufakwa kwimidiya yokukhuliswa kwamaseli njenge-ejenti yokuvimba ukuze kulondolozwe izimo ze-pH ezifanele zokukhula kwamaseli. Ukuhambisana kwayo nezinto eziphilayo kanye nokuqina kwayo kwenza kube usizo ezinhlotsheni ezahlukene zamaseli kokubili ocwaningweni kanye nasezisetshenzisweni zezimboni.

I-Electrophoresis: I-TAPS ingasetshenziswa njenge-ejenti yokubopha kumasu e-gel electrophoresis, njenge-SDS-PAGE (Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis). Kuyasiza ukugcina i-pH ezinzile ngesikhathi sokuhlukaniswa nokuhlaziywa kwamaprotheni, ama-nucleic acid, kanye namanye ama-biomolecule.