I-L-Leucine p-nitroanilide hydrochloride CAS:16010-98-3

I-L-Leucine p-nitroanilide hydrochloride ivame ukusetshenziswa njenge-substrate ocwaningweni lwe-biochemical kanye nokuhlolwa kwe-enzyme ukukala umsebenzi wama-enzyme ahlukahlukene, ikakhulukazi ama-protease nama-peptidases. Lapho la ma-enzyme ehlukanisa i-substrate, ikhipha i-p-nitroaniline, engatholakala nge-spectrophotometric.

Izinga lokushintsha kombala lihambisana ngqo nomsebenzi we-enzyme, okuvumela ukulinganiswa komsebenzi we-enzyme kumasampula ebhayoloji. Lokhu kwenza i-L-Leucine p-nitroanilide hydrochloride ibe ithuluzi eliwusizo lokufunda i-enzyme kinetics, ukuhlola ama-enzyme inhibitors noma ama-activator, kanye nokuhlola imiphumela yezimo zokuhlola noma ukungenelela emsebenzini we-enzyme.

Ukuzwela kwe-L-Leucine p-nitroanilide hydrochloride ekuhlolweni okuhlukene kwe-enzyme kungahluka, kudinga ukwenziwa ngcono kwezinhlelo ezithile. Ngokuvamile isetshenziswa ngamanani asukela ku-0.5 mM kuya ku-5 mM, kuye nge-enzyme ethile kanye nezimo zokuhlola.