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I-Dodecyl trimethyl ammonium bromide CAS:1119-94-4
I-Dodecyl trimethyl ammonium bromide, eyaziwa nangokuthi i-DTAB noma i-DTB, iyinhlanganisela ye-ammonium ye-quaternary evame ukusetshenziswa njenge-surfactant noma insipho. Iyimpuphu emhlophe, eqinile encibilikayo emanzini.
I-DTAB ivame ukusetshenziswa ezimbonini nasezinhlelweni ezahlukahlukene, okuhlanganisa imikhiqizo yokunakekela umuntu siqu, imithi, kanye namalebhu ocwaningo. Ingasebenza njenge-ejenti yokuhlanza, i-emulsifier, i-ejenti ye-foam, noma i-ejenti yokumanzisa.
Emikhiqizweni yokunakekela umuntu siqu, i-DTAB ingatholakala kuma-shampoo, kuma-conditioner, ensipheni, nasezihlanzweni zomzimba. Iyasiza ekwakheni igwebu, yandise ukuzinza komkhiqizo, futhi ithuthukise ukusabalala kwefomula.
Kwezemithi, i-DTAB isetshenziswa ekwakhiweni kwezinhlelo ezahlukene zokulethwa kwemithi, okuhlanganisa ama-microemulsion, ama-nanoemulsion, kanye nama-liposome. Isiza ekuncibilikisweni kwemithi kanye nokuzinzisa, ithuthukise ukumuncwa kwemithi kanye nokusebenza kahle kwayo.
Emalabhorethri ocwaningo, i-DTAB ivame ukusetshenziswa ezifundweni zezinto eziphilayo kanye nezamakhemikhali. Ingasetshenziswa ekukhipheni nasekuhlanzeni amaprotheni e-membrane, ekukhipheni i-DNA, kanye nezinhlelo zokulethwa kwezakhi zofuzo.
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I-PMSF CAS:329-98-6 Intengo Yomkhiqizi
I-Phenylmethylsulfonyl fluoride, eyaziwa nangokuthi i-PMSF, iyinhlanganisela yamakhemikhali evame ukusetshenziswa njenge-serine protease inhibitor. Iyisivimbeli esisebenzayo nesinamandla esingaguquki esisebenza ngokubopha nokuguqula indawo esebenzayo yama-serine protease, ivimbele ngempumelelo umsebenzi wawo we-enzyme. I-PMSF iwusizo kakhulu ocwaningweni lwe-biochemical kanye ne-molecular biology lokufunda ukuhlanzwa kwamaprotheni, ukusebenzisana kwamaprotheni nama-protein, kanye ne-proteolysis. Ivame ukusetshenziselwa ukuvikela amaprotheni ekuwohlokeni ngesikhathi sezinqubo zokuhlanza nokuvimbela umsebenzi we-protease kuma-lysates nama-extracts eseli.
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I-Ethylenediaminetetraacetic acid i-dipotassium salt dihydrate I-CAS:25102-12-9
I-Ethylenediaminetetraacetic acid i-dipotassium salt dihydrate, eyaziwa nangokuthi i-EDTA Dipotassium, iyinhlanganisela yamakhemikhali enefomula yama-molecule i-K2(C10H14N2O8)·2H2O. Iyimpuphu ekristalu evame ukusetshenziswa njenge-ejenti yokuchela, okusho ukuthi ingabopha kuma-ion ensimbi. I-EDTA Dipotassium yaziwa ngekhono layo lokususa noma ukususa ama-ion ensimbi ezixazululweni, okwenza ibe usizo ezinqubweni ezahlukahlukene zezimboni, njengokwelashwa kwamanzi, ukucubungula ukudla neziphuzo, kanye nokwakheka kwemithi. Iphinde isetshenziswe kumakhemikhali okuhlaziya kanye nokwelashwa kwezokwelapha ngenxa yezakhiwo zayo zokuchela insimbi.
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I-Ethylenediaminetetraacetic acid tripotassium salt dihydrate CAS:65501-24-8
I-Ethylenediaminetetraacetic acid i-tripotassium salt dihydrate, eyaziwa nangokuthi i-EDTA Tripotassium, iyinhlanganisela equkethe ama-ion amathathu e-potassium (K+) nge-molecule ngayinye, kanye nama-molecule amabili amanzi (H2O). I-EDTA Tripotassium iyi-derivative ye-ethylenediaminetetraacetic acid (EDTA) futhi inezakhiwo ezifanayo kanye nezicelo.
I-EDTA Tripotassium isetshenziswa kakhulu njenge-chelating agent, okusho ukuthi inamandla okubopha kuma-ion ensimbi futhi yakhe ama-complexes azinzile. Ivame ukusetshenziswa ukususa ama-ion ensimbi angafuneki ezixazululweni, ikakhulukazi i-calcium, i-magnesium, kanye namanye ama-ion ensimbi esindayo. Ngokuhlukanisa lawa ma-ion, i-EDTA Tripotassium ingavimbela ukuphazamiseka kwawo noma imiphumela emibi ezinqubweni nasezindleleni ezahlukahlukene zamakhemikhali.
Ngaphezu kwezakhiwo zayo zokuchela, i-EDTA Tripotassium ithola nezicelo ezimbonini ezahlukahlukene. Ivame ukusetshenziswa ekuphatheni amanzi ukususa ama-ion ensimbi esindayo emithonjeni yamanzi, ukuqinisekisa ukuphepha kwamanzi kanye nekhwalithi. Ingatholakala futhi embonini yokudla neziphuzo njengesiqinisi kanye nesilondolozi, ivimbela ukubola kwe-oxidation kanye nokugcina ikhwalithi yokudla okucutshungulwayo. Ngaphezu kwalokho, i-EDTA Tripotassium idlala indima ekwakhiweni kwemithi, ikhemistri yokuhlaziya, kanye nezinto ezijwayelekile zokubhekisela.
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I-Fluorescein Isothiocyante CAS:3326-32-7
I-Fluorescein isothiocyanate (FITC) iwudayi we-fluorescent ovame ukusetshenziswa ocwaningweni lwezokwelapha kanye nezicelo zokuxilonga. Iwuhlobo lwe-fluorescein olushintshiwe ukuze luqukathe iqembu le-isothiocyanate, elivumela ukuthi libopheke ndawonye kumaprotheni, ama-antibodies, kanye namanye ama-biomolecule.
I-FITC ikhipha ukukhanya okuluhlaza okukhanyayo uma ishukunyiswa ukukhanya kobude be-wavelength obufanele, ngokuvamile obungu-488 nm. Lokhu kukhanya kungatholakala futhi kulinganiswe kusetshenziswa amasu ahlukahlukene okuthwebula izithombe, njenge-fluorescence microscopy, i-flow cytometry, kanye ne-fluorescence spectroscopy.
Ngokuhlanganisa i-FITC nama-biomolecule athakazelisayo, abacwaningi bangalandelela futhi babone ngeso lengqondo ukusatshalaliswa, indawo, kanye nokusebenzisana kwala ma-biomolecule ngaphakathi kwamaseli, izicubu, noma amasampula ezinto eziphilayo. Ama-antibodies anelebula le-FITC, isibonelo, angasetshenziswa ukuthola amaprotheni noma ama-antigen athile ekuhlolweni kwe-immunofluorescence noma ukuhlaziya inani lamaseli ekuhlolweni kwe-flow cytometry.
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I-GSH CAS: 70-18-8 Intengo Yomkhiqizi
I-GSH, noma i-glutathione, iyi-molecule yemvelo ye-antioxidant etholakala emzimbeni. Idlala indima ebalulekile ekugcineni ukusebenza kahle kwamaseli futhi ihilelekile ezinqubweni eziningana ezibalulekile zomzimba. I-GSH isiza ukuvikela amaseli ekulimaleni kwe-oxidative ngokunciphisa ama-free radical ayingozi kanye nezinhlobo ze-oxygen ezisabelayo. Iphinde isize ezinqubweni zokukhipha ubuthi ngokubopha nokususa ubuthi nezinsimbi ezisindayo emzimbeni.
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I-BCA-2Na CAS:979-88-4 Intengo Yomkhiqizi
I-BCA-2Na, eyaziwa nangokuthi i-Bacillus cereus adenosine triphosphate (ATP) bioluminescent assay, iyisivivinyo se-biochemical esisetshenziselwa ukuthola ukuthi kukhona i-ATP kusampula. I-ATP iyi-molecule etholakala kuzo zonke izinto eziphilayo futhi ivame ukusetshenziswa njengesibonakaliso sokungcoliswa noma ukuhlanzeka kwamagciwane. Isivivinyo se-BCA-2Na sisebenzisa i-enzyme luciferase ukuze sivuselele ukusabela okukhiqiza ukukhanya lapho kukhona i-ATP. Ukuqina kokukhanya okukhishwayo kuyalinganiswa futhi kunganikeza ukuhlolwa okusheshayo nokubucayi komsebenzi wamagciwane noma ukuhlanzeka ezimbonini ezahlukahlukene, njengokukhiqizwa kokudla neziphuzo, ukukhiqizwa kwemithi, kanye nokuhlolwa kwekhwalithi yamanzi. Isivivinyo se-BCA-2Na sivame ukusetshenziswa njengendlela esheshayo nethembekile yokuqapha inhlanzeko kanye nokulawula ikhwalithi.
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I-GSSG CAS:27025-41-8 Intengo Yomkhiqizi
I-GSSG, eyaziwa nangokuthi i-oxidized glutathione, iwuhlobo lwe-disulfide lwe-antioxidant glutathione (GSH). Ngenkathi i-GSH iwuhlobo olunciphile nolusebenzayo, i-GSSG iwuhlobo lwe-oxidized nolungasebenzi. I-GSSG yakheka lapho i-GSH inikela ngama-electron ayo ukuze iqede ama-free radical noma ezinye izinhlobo ezisabelayo. Lokhu kuguqulwa kusiza ukuvikela umzimba ekulimaleni kwe-oxidative. I-GSSG ingaguqulwa ibuyele ku-GSH ngenqubo yokuvuselela ehilela i-enzyme glutathione reductase. Ukuqapha isilinganiso se-GSH kuya ku-GSSG emzimbeni kuyasiza ekuhloleni isimo se-antioxidant kanye nempilo iyonke.
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I-BCIP Na) 5-Bromo-4-chloro-3-indolyl phosphate disodium salt CAS:102185-33-1
Usawoti we-disodium we-5-Bromo-4-chloro-3-indolyl phosphate uyinhlanganisela yokwenziwa evame ukusetshenziswa ocwaningweni lwe-biochemical njenge-substrate ye-chromogenic yokuthola umsebenzi wama-enzyme e-alkaline phosphatase. Ngokuvamile ubizwa ngokuthi i-BCIP ngamafuphi.
Uma i-alkaline phosphatase isebenza ku-BCIP, ihlukanisa iqembu le-phosphate, okuholela ekwakhekeni kwe-precipitate eluhlaza okwesibhakabhaka. Lokhu kushintsha kombala kuvumela abacwaningi ukuthi babone futhi balinganise ukuba khona kwe-alkaline phosphatase kusampula.
I-BCIP ivame ukusetshenziswa ezinhlotsheni ezahlukahlukene, kufaka phakathi amasu okuhlola i-immunohistochemistry, amasu okuhlola i-protein kanye ne-nucleic acid, kanye nokuhlolwa kwe-immunosorbent okuxhunywe yi-enzyme (ELISAs).
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Iodoacetamide CAS:144-48-9 Intengo Yomkhiqizi
I-Iodoacetamide iyinhlanganisela yamakhemikhali evame ukusetshenziswa ocwaningweni lwe-biochemistry kanye ne-molecular biology. Iyinhlanganisela esabelayo ye-thiol eguqula ngqo izinsalela ze-cysteine kumaphrotheni ngokwenza isibopho esihlanganisiwe. Lokhu kuguqulwa kuvame ukusetshenziswa emasu okuhlaziya amaprotheni ukuvimba izinsalela ze-cysteine, njengasezifundweni ze-proteomics lapho isetshenziswa khona ukulebula nokuvimba izinsalela ze-cysteine ngokukhetha ngaphambi kokugaya amaprotheni kanye nokuhlaziywa kwe-mass spectrometry. I-Iodoacetamide isetshenziswa futhi ezifundweni zesakhiwo samaprotheni kanye nasekuhlanganisweni kwama-peptide anelebula..
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I-BCIP-Toluidine)5-Bromo-4-chloro-3-indolylphosphate-p-toluidine usawoti i-CAS:6578-06-9
Usawoti we-5-Bromo-4-chloro-3-indolyl phosphate p-toluidine uyinhlanganisela yamakhemikhali evame ukusetshenziswa ocwaningweni lwe-molecular biology kanye ne-biochemistry. Uvame ukusetshenziswa ekuhlolweni kwe-enzyme-linked immunosorbent assays (ELISA) kanye namasu okufaka ilebula lamaprotheni.
Le nhlanganisela iyi-substrate ye-alkaline phosphatase, i-enzyme esetshenziswa kabanzi kwi-biology yama-molecule ukuthola ubukhona bama-molecule noma amaprotheni athile ngokusebenzisa ukusabela kwe-colorimetric noma kwe-chemiluminescent.
Uma i-enzyme ye-alkaline phosphatase ikhona, ihlukanisa amaqembu e-phosphate kusawoti we-5-Bromo-4-chloro-3-indolyl phosphate p-toluidine, okuholela ekwakhekeni kodayi oluhlaza okwesibhakabhaka noma onsomi. Lokhu kushintsha kombala kungabonakala kalula futhi kulinganiswe, okuvumela ukukalwa komsebenzi we-enzyme noma ukuba khona kwama-molecule aqondiwe.
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I-N,N-Dimethyl-1,4-phenylenediamine dihydrochloride I-CAS:536-46-9
I-N,N-Dimethyl-1,4-phenylenediamine dihydrochloride iyinhlanganisela yamakhemikhali evame ukubizwa ngokuthi i-DMPD dihydrochloride. Yinto eqinile, ekristalu encibilikayo emanzini nakwezinye izinyibilikisi ezibandayo.
I-DMPD dihydrochloride ivame ukusetshenziswa ezinhlobonhlobo zezindlela zokusabela kwamakhemikhali nezinqubo, okuhlanganisa ukusabela kwe-oxidation kanye nokunciphisa. Ingasebenza njengomthombo wama-electron noma njengesamukeli sama-electron, okwenza kube usizo ekwakhiweni kwamadayi, imithi, kanye nezinye izinto eziphilayo.
Ngaphezu kokusebenza kwayo ngendlela ehlelekile, i-DMPD dihydrochloride yaziwa ngezakhiwo zayo zokunciphisa ezinamandla. Ingasetshenziswa njengesinciphisi kumasu e-analytical chemistry, njenge-spectrophotometry, lapho isiza khona ukulinganisa ukuhlushwa kwezinto ezithile.
