I-BCIP Na) 5-Bromo-4-chloro-3-indolyl phosphate disodium salt CAS:102185-33-1

Umphumela:

I-BCIP iyinhlanganisela engenambala eguqulwa ibe yi-precipitate eluhlaza okwesibhakabhaka ngokusebenza kwe-alkaline phosphatase. Lokhu kushintsha kombala kuvumela ukubona okubonakalayo kanye nokulinganisa umsebenzi we-enzyme.

Izicelo:

Ukuhlolwa kwe-enzyme: I-BCIP isetshenziswa ekuhlolweni okuhlukahlukene kwamakhemikhali ukuze kulinganiswe umsebenzi we-alkaline phosphatase. Lokhu kuhlanganisa ukuthola umsebenzi we-phosphatase kuma-lysates eseli, ama-enzyme ahlanziwe, noma ukuhlolwa kwe-in vitro.

I-Immunohistochemistry: I-BCIP ingasetshenziswa ukubona ngeso lengqondo ama-antibodies abhalwe nge-alkaline phosphatase ezingxenyeni zezicubu noma ebusweni bamaseli. Le ndlela ivame ukusetshenziswa kumaphrothokholi okuvikela amasosha omzimba.

Amasu okufiphaza: I-BCIP isetshenziswa ku-Western blotting, Southern blotting, kanye ne-Northern blotting ukuthola amaprotheni athile noma ama-nucleic acid abhalwe ngama-probe ahlanganiswe ne-alkaline phosphatase.

Ukuxutshwa kwezakhi zofuzo ezitholakala endaweni eyodwa: I-BCIP ingasetshenziswa ukubona ngeso lengqondo amaphethini okubonakaliswa kwezakhi zofuzo ezicutshini noma emaseli ngezindlela zokuxutshwa kwezakhi zofuzo ezitholakala endaweni eyodwa.

Ukuhlolwa kokuxilonga: I-BCIP isetshenziswa njengesisekelo kuma-kit ahlukahlukene okuxilonga ukuze kutholakale i-alkaline phosphatase. Lawa ma-kit asetshenziswa emalabhorethri emitholampilo ukuze kutholakale futhi kuqashwe izifo.