I-PMSF CAS:329-98-6 Intengo Yomkhiqizi

Umphumela:

I-PMSF iyisithiyo se-serine protease esibopha ngokungenakulungiseka endaweni esebenzayo yama-serine protease, ngaleyo ndlela sivimbele umsebenzi wawo.

Iguqula izinsalela ze-serine ngezibopho ze-covalent, okuholela ekungasebenzini kwe-enzyme.

I-PMSF ingavikela amaprotheni ekuwohlokeni ngokuvimbela umsebenzi we-protease, ikakhulukazi ama-serine protease.

Izicelo:

Ucwaningo lwe-biochemical: I-PMSF ivame ukusetshenziswa ngesikhathi sokuhlanzwa kwamaprotheni kanye nezinqubo zokuhlukanisa ukuvimbela ukuwohloka kwamaprotheni. Kuyasiza ekugcineni ubuqotho kanye nokuqina kweprotheni eqondiwe.

Ukuvinjelwa kwama-enzyme: I-PMSF iyi-reagent ebalulekile yokuvimbela ama-serine protease ngesikhathi sokuhlolwa kwama-enzyme noma izifundo ze-biochemical. Ngokuvimba umsebenzi wala ma-protease, abacwaningi bangaphenya indima yama-serine protease ezinqubweni ezahlukahlukene zezinto eziphilayo.

Izifundo ze-Proteolysis: I-PMSF ingasetshenziswa ukulawula noma ukulungisa umsebenzi we-protease kuma-lysates noma kuma-extracts eseli. Lokhu kuwusizo ekutadisheni izehlakalo ze-proteolytic ezihilelekile ezinqubweni zamaseli noma lapho i-proteolysis idinga ukulawulwa ekusethweni kokuhlola.

Ukuvinjelwa kwe-protease okujwayelekile: Nakuba i-PMSF ihlose ngqo ama-serine protease, ingaba nomthelela othile ekuvimbeleni kwamanye ama-cysteine ​​protease. Isetshenziswa njengesivimbeli se-protease esijwayelekile lapho isebenza ngamasampula eprotheyini ukunciphisa ukuwohloka kwe-proteolytic.