I-BCIP-Toluidine)5-Bromo-4-chloro-3-indolylphosphate-p-toluidine usawoti i-CAS:6578-06-9

Usawoti we-5-Bromo-4-chloro-3-indolyl phosphate p-toluidine uyinhlanganisela yamakhemikhali evame ukusetshenziswa ocwaningweni lwe-molecular biology kanye ne-biochemistry. Uvame ukusetshenziswa ekuhlolweni kwe-enzyme-linked immunosorbent assays (ELISA) kanye namasu okufaka ilebula lamaprotheni.

Le nhlanganisela iyi-substrate ye-alkaline phosphatase, i-enzyme esetshenziswa kabanzi kwi-biology yama-molecule ukuthola ubukhona bama-molecule noma amaprotheni athile ngokusebenzisa ukusabela kwe-colorimetric noma kwe-chemiluminescent.

Uma i-enzyme ye-alkaline phosphatase ikhona, ihlukanisa amaqembu e-phosphate kusawoti we-5-Bromo-4-chloro-3-indolyl phosphate p-toluidine, okuholela ekwakhekeni kodayi oluhlaza okwesibhakabhaka noma onsomi. Lokhu kushintsha kombala kungabonakala kalula futhi kulinganiswe, okuvumela ukukalwa komsebenzi we-enzyme noma ukuba khona kwama-molecule aqondiwe.

Oda noma Cela Isilinganiso

Imininingwane Yomkhiqizo

Amathegi Omkhiqizo

Ukusetshenziswa kanye Nomphumela

Ukuqothula kwe-Western: I-BCIP isetshenziselwa ukuthola nokubona ngeso lengqondo amaprotheni adluliselwa kuma-membrane ngemuva kwe-gel electrophoresis. Ama-antibodies esibili ahlanganiswe ne-alkaline phosphatase asetshenziselwa ukubopha kuma-antibodies ayinhloko, futhi ukufukamela okulandelayo nge-BCIP kuphumela ekwakhiweni kwe-blue precipitate endaweni yama-protein band.

I-Immunohistochemistry: I-BCIP isetshenziswa kumasu okufaka umbala we-immunohistochemical ukuthola ukuthi kukhona amaprotheni athile ezicutshini. Ama-antibodies esibili ahlanganiswe ne-alkaline phosphatase asetshenziselwa ukubopha kuma-antibodies ayinhloko, kanti ukwelashwa kwe-BCIP kukhiqiza umbala oluhlaza okwesibhakabhaka lapho kukhona i-enzyme, okubonisa indawo yeprotheni ngaphakathi kwesampula yezicubu.

I-in situ hybridization: I-BCIP isetshenziselwa futhi ukuthola ukulandelana kwe-nucleic acid ezicutshini noma emaseli aqinile. Ama-hybridization probes abhalwe nge-alkaline phosphatase, futhi uma kungezwa i-BCIP, ukuba khona kwe-target mRNA noma i-DNA kuboniswa ukuthuthukiswa kombala oluhlaza okwesibhakabhaka.

Ukupakisha Umkhiqizo:

Ulwazi Olwengeziwe:

Ukwakheka	C8H6BrClNO4P.C7H9N
Ukuhlolwa	99%
Ukubukeka	Impuphu emhlophe
Inombolo ye-CAS	6578-06-9
Ukupakisha	Encane futhi enkulu
Impilo Yeshelufu	iminyaka engu-2
Isitoreji	Gcina endaweni epholile neyomile
Isitifiketi	I-ISO.