Iodoacetamide CAS:144-48-9 Intengo Yomkhiqizi

Ukuguqulwa kwe-Cysteine: I-Iodoacetamide isabela namaqembu e-thiol yamahhala kuma-residues e-cysteine, yakha isibopho esihambisanayo ne-cysteine ​​​​sulfur. Lokhu kuguqulwa kusiza ekuhlaziyweni kwamaprotheni ngokuvimbela ama-residues e-cysteine ​​​​nokuvimbela ukusabela okungafuneki noma i-oxidation.

Ukugaya Amaprotheni: I-Iodoacetamide ivame ukusetshenziswa emisebenzini yokugaya amaprotheni yokuhlaziywa kwe-mass spectrometry. Isabela nezinsalela ze-cysteine ​​​​ngaphambi kokugaya ama-enzyme, ivimbele ukwakheka kwesibopho se-disulfide okungafuneki futhi igcine iphrotheni isesimweni sayo esinciphile.

Izifundo ze-Proteomics: I-Iodoacetamide isetshenziswa emasu okulebula ahilela i-proteomics esekelwe ku-mass spectrometry. Ngokusabela kwayo nama-cysteine ​​residues, ivumela ukulebula okukhethiwe kanye nokuhlonza ama-peptide aqukethe i-cysteine.

Izifundo Zesakhiwo Samaprotheni: I-Iodoacetamide isetshenziswa ezifundweni zesakhiwo ukuguqula amakhemikhali ama-cysteine ​​​​residues futhi ifunde umsebenzi wawo kanye nokubandakanyeka kwawo ekugoqeni, ekubopheni, kanye nasekushintsheni kwesimo samaprotheni. Ingasiza futhi ekuhlolweni kokuxhumanisa ukuze kuhlolwe ukusebenzisana kwamaprotheni namaphrotheni.

Ukwenziwa kwama-Peptide Anelebula: I-Iodoacetamide ingasetshenziswa ukuhlanganisa ama-peptide anelebula ngokushintsha izinsalela ze-cysteine ​​​​ngama-compounds anelebula eliyi-isotopic, njenge-deuterium noma i-carbon-13. Lawa ma-peptide anelebula abe esesetshenziswa ezicelweni ezahlukene zocwaningo, okuhlanganisa ukulinganiswa kwamaprotheni kanye nezifundo ze-dynamics.