I-Fluorescein Isothiocyante CAS:3326-32-7

Izakhiwo ze-Fluorescence: I-FITC ine-quantum vield ephezulu futhi ikhipha i-fluorescence eluhlaza okwesibhakabhaka lapho ivuselelwa cishe ku-488 nm. Izakhiwo zayo ze-fluorescence ziyenza ifaneleke ukutholakala kusetshenziswa amasu ahlukahlukene okuthwebula izithombe, njenge-fluorescence microscopy, i-flow cytometry, kanye ne-fluorescence spectroscopy.

Ukubopha kwe-Covalent: I-FITC iqukethe iqembu le-isothiocyanate eliyivumela ukuthi ibophe ndawonye emaqenjini ama-amino akhona kumaphrotheni, ama-antibodies, kanye namanye ama-biomolecule. Lokhu kubopha kwe-covalent kuqinisekisa ukulebula okuzinzile nokuqondile kwama-molecule aqondiwe, okuvumela ukutholakala kwawo nokubonakala kwawo.

I-Immunofluorescence: Ama-antibodies anelebula le-FITC avame ukusetshenziswa ekuhlolweni kwe-immunofluorescence ukuthola ama-antigen athile noma amaprotheni kumaseli nezicubu. I-fluorescence eluhlaza okotshani ekhishwa yi-FITC ivumela abacwaningi ukuthi babone futhi babone ukusatshalaliswa kwama-molecule aqondiwe ngaphakathi kwamasampula ebhayoloji.

I-Flow cytometry: Ama-antibodies anelebula le-FITC noma amanye ama-biomolecule ahlanganiswe ne-fluorochrome asetshenziswa kabanzi ku-flow cytometry ukuhlaziya nokuhlunga amaseli ngokusekelwe kuma-surface markers awo noma izingxenye zangaphakathi kweseli. I-FITC iyahambisana ne-laser engu-488 nm esetshenziswa kakhulu kumathuluzi e-flow cytometry.

Izifundo zokusebenzisana kwamaprotheni: I-FITC ingasetshenziswa ezifundweni zokusebenzisana kwamaprotheni ukuhlola ukubopha nokusebenzisana kwamaprotheni. Ngokufaka amalebula amaprotheni athile nge-FITC, abacwaningi bangahlaziya ukusebenzisana kwawo besebenzisa amasu afana nokudluliselwa kwamandla e-fluorescence resonance (FRET) noma i-fluorescence polarization.

Ukulandelela ama-biomolecule: I-FITC ingasetshenziswa ukulandelela nokulandelela ukunyakaza kwama-biomolecule ngaphakathi kwamaseli noma izicubu eziphilayo. Ngokufaka amalebula kuma-molecule athakazelisayo nge-FITC, abacwaningi bangafunda indawo yawo, amandla awo, kanye namaphethini okushushumbisa ngesikhathi sangempela besebenzisa i-fluorescence microscopy.

Izicelo zokuxilonga: Ama-probe noma ama-antibodies anelebula le-FITC asetshenziswa ekuxilongeni izinhlelo zokusebenza ezahlukahlukene, okuhlanganisa ukutholakala kwama-antigen athile, izinto ezithathelwanayo, noma ama-biomarker kumasampula eziguli. La ma-probe akhanyayo ahlinzeka ngokutholakala okubucayi nokuqondile, okuvumela ukuxilonga okusheshayo nokunembile.