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I-PNPG CAS:3150-24-1 Intengo Yomkhiqizi
I-PNPG, noma i-p-nitrophenyl β-D-glucopyranoside, i-substrate ye-molecule encane evame ukusetshenziswa ekuhlolweni kwamakhemikhali e-biochemical ukukala umsebenzi wama-enzyme e-glucosidase.Ayinambala futhi ayinafluorescent, kodwa uma i-hydrolysis yi-glucosidase, iguqulwa ibe i-p-nitrophenol, enombala ophuzi futhi ibonakala kalula nge-spectrophotometrically.
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I-ONPG CAS:369-07-3 Intengo Yomkhiqizi
I-O-nitrophenyl-β-D-galactopyranoside (ONPG) iyi-substrate yokwenziwa esetshenziswa ekuhlolweni kwebhayoloji ye-biochemical kanye ne-molecular biology ukukala umsebenzi we-enzyme i-β-galactosidase.Ngokuvamile isetshenziswa ekuhlolweni ukuze kutholwe ukubonakaliswa kofuzo ezinhlelweni zebhaktheriya, njenge-Escherichia coli. I-ONPG iyinhlanganisela engenambala eqhekezwa yi-β-galactosidase, okuholela ekukhishweni kwenhlanganisela ephuzi, i-o-nitrophenol.Umbala ophuzi okhiqizwayo ungakalwa nge-spectrophotometrically, unikeze isilinganiso esingaqondile somsebenzi we-enzyme.Isivivinyo esisetshenziswa i-ONPG sivame ukubizwa ngokuthi i-ONPG assay futhi kuyindlela esetshenziswa kakhulu ocwaningweni lwebhayoloji yamangqamuzana ukuhlola amazinga okuveza izakhi zofuzo ezilawulwa yi-lac. i-operon kumaseli webhaktheriya.
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I-Nitrotetrazolium Blue Chloride CAS: 298-83-9
I-Nitrotetrazolium Blue Chloride (NBT) iyinkomba ye-redox evame ukusetshenziswa ekuhlolweni kwebhayoloji ne-biochemical.Kuyimpushana ephuzi ephaphathekile ephenduka ibe luhlaza okwesibhakabhaka lapho incishisiwe, ikwenze kube usizo ekutholeni ukuba khona kwama-enzyme athile kanye nomsebenzi we-metabolic.
I-NBT isabela ngezithwali zama-electron nama-enzyme afana nama-dehydrogenases, abandakanyeka ezinqubweni ezihlukahlukene zamaselula.Uma i-NBT yehliswa yilawa ma-enzyme, yenza i-formazan precipitate eluhlaza okwesibhakabhaka, evumela ukutholwa okubonakalayo noma kwe-spectrophotometric.
Lesi sici sivame ukusetshenziswa ekuhlolweni okufana nokuhlolwa kokunciphisa i-NBT, lapho sisetshenziselwa ukuhlola umsebenzi wokuqhuma kokuphefumula kwamaseli omzimba.Ingasetshenziswa futhi ukutadisha imisebenzi ye-enzyme kanye nezindlela ze-metabolic ocwaningweni oluphathelene nengcindezi ye-oxidative, ukusebenza kahle kwamaseli, kanye nokuhlukaniswa kwamaseli.
I-NBT ithole izinhlelo zokusebenza emikhakheni eyahlukene, okuhlanganisa i-microbiology, i-immunology, kanye ne-cell biology.Iguquguquka, izinzile, futhi kulula ukuyisebenzisa, okuyenza ibe ukukhetha okudumile kumaphrothokholi amaningi okuhlola.
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I-Neocuproine CAS:484-11-7 Intengo Yomkhiqizi
I-Neocuproine i-ejenti ye-chelating evame ukusetshenziswa ezinhlelweni ezihlukahlukene, kuhlanganise namakhemikhali okuhlaziya kanye nemithi.Inobudlelwane obuqinile be-ion yethusi futhi yenza ama-complexes azinzile nawo.Lesi sakhiwo senza i-neocuproine ibe usizo ekutholeni nasekulinganiseni ithusi kuzixazululo noma amasampula.Ukwengeza, i-neocuproine iye yafundelwa izinhlelo zayo zokwelapha ezingaba khona, ikakhulukazi ekwelapheni umdlavuza kanye nezifo ze-neurodeergenerative.
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IPTG CAS:367-93-1 Intengo Yomkhiqizi
I-Isopropyl β-D-1-thiogalactopyranoside (IPTG) iyi-analog yokwenziwa ye-lactose evame ukusetshenziswa ocwaningweni lwebhayoloji yamangqamuzana nasekusetshenzisweni kwe-biotechnology.I-IPTG isetshenziselwa kakhulu ukunxenxa ukuvezwa kofuzo ezinhlelweni zebhaktheriya, lapho isebenza khona njengesibasi samangqamuzana ukuqalisa ukulotshwa kofuzo oluqondiwe.
Uma ingezwe endaweni yokukhula, i-IPTG ithathwa yibhaktheriya futhi ingakwazi ukubopha iphrotheni ye-lac repressor, ukuyivimbela ekuvimbeleni umsebenzi we-lac operon.I-lac operon iyiqoqo lezakhi zofuzo ezihilelekile ku-lactose metabolism, futhi lapho iphrotheni ye-repressor isusiwe, izakhi zofuzo ziyavezwa.
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I-HATU CAS:148893-10-1 Intengo Yomkhiqizi
I-HATU (1--[bis(dimethylamino))methylene]-1H-1,2,3-triazolo[4,5-b]pyridinium 3-oxid hexafluorophosphate) iyi-reagent yokuhlanganisa evame ukusetshenziswa ku-peptide synthesis kanye ne-organic chemistry.
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I-D-fucose CAS:3615-37-0 Intengo Yomkhiqizi
I-D-fucose iyi-monosaccharide, ikakhulukazi ushukela wekhabhoni eyisithupha, eyingxenye yoshukela olula obizwa ngokuthi ama-hexoses.I-isomer ye-glucose, ehlukile ekumisweni kweqembu elilodwa le-hydroxyl.
I-D-fucose ngokwemvelo itholakala ezintweni eziphilayo ezihlukahlukene, okuhlanganisa amagciwane, isikhunta, izitshalo nezilwane.Idlala izindima ezibalulekile ezinqubweni ezimbalwa zebhayoloji, njengokusayina kwamaseli, ukunamathela kwamaseli, kanye nokuhlanganiswa kwe-glycoprotein.Iyingxenye yama-glycolipids, ama-glycoprotein, nama-proteoglycans, abambe iqhaza ekuxhumaneni kwe-cell-to-cell kanye nokuqashelwa.
Kubantu, i-D-fucose iphinde ihileleke ku-biosynthesis yezakhiwo ezibalulekile ze-glycan, njenge-Lewis antigens nama-antigens eqembu legazi, okunomthelela ekusebenzisaneni nokumpontshelwa igazi kanye nokuba sengozini kwezifo.
I-D-fucose ingatholakala emithonjeni ehlukahlukene, kuhlanganise nezimila zasolwandle, izitshalo, kanye nokuvutshelwa kwe-microbial.Isetshenziselwa ucwaningo kanye nezicelo ze-biomedical, kanye nokukhiqizwa kwemithi ethile kanye nezinhlanganisela zokwelapha.
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I-DDT CAS:3483-12-3 Intengo Yomkhiqizi
I-DL-Dithiothreitol, eyaziwa nangokuthi i-DTT, iyi-ejenti yokunciphisa evame ukusetshenziswa ocwaningweni lwebhayoloji yamakhemikhali namangqamuzana.Kuyi-molecule encane eneqembu le-thiol (eliqukethe isulfure) ekugcineni ngakunye.
I-DTT ivame ukusetshenziselwa ukunqamula izibopho ze-disulfide kumaprotheni, esiza ukuwavula noma ukuwaguqula.Lokhu kwehliswa kwamabhondi e-disulfide kubalulekile ezinqubweni zaselabhorethri ezihlukahlukene ezifana nokuhlanzwa kwamaprotheni, i-gel electrophoresis, nezifundo zesakhiwo samaprotheni.I-DTT ingasetshenziswa futhi ukuvikela amaqembu e-thiol nokuvimbela i-oxidation phakathi nezinqubo zokuhlola.
I-DTT ivamise ukungezwa ezixazululweni zokuhlola ezindaweni ezincane, futhi umsebenzi wayo uncike ekubeni khona komoyampilo.Kubalulekile ukuphatha i-DTT ngokucophelela njengoba izwela emoyeni, ukushisa, nomswakama, okunganciphisa ukusebenza kwayo.
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I-D-(+)-Galactose CAS:59-23-4 Intengo Yomkhiqizi
I-D-(+)-I-Galactose ingushukela we-monosaccharide kanye nengxenye ebalulekile yezinqubo eziningi zezinto eziphilayo.Iwushukela ovela ngokwemvelo otholakala ekudleni okuningi, njengezithelo, imikhiqizo yobisi, nemifino.
I-Galactose ivamise ukwenziwa i-metabolized emzimbeni ngochungechunge lokusabela kwe-enzymatic.Idlala indima ebalulekile ekuxhumaneni kwamangqamuzana, ukukhiqizwa kwamandla, kanye ne-biosynthesis yama-molecule abalulekile njenge-glycolipids, i-glycoprotein, ne-lactose.
Ngokwezinhlelo zayo zokusebenza, i-D-(+)-Galactose ivame ukusetshenziswa ku-microbiology kanye ne-biotechnology njengomthombo wekhabhoni emithonjeni yamasiko ukuze kukhule amagciwane ahlukahlukene.Ibuye isetshenziswe ekukhiqizeni izinhlanganisela ezihlukahlukene ze-bioactive, imithi, nemikhiqizo yokudla.Ukwengeza, isetshenziswa kaningi njenge-ejenti yokuxilonga yezokwelapha, ikakhulukazi ekuhlolweni kokuhlola ukusebenza kwesibindi nokuthola ukuphazamiseka kofuzo okuhlobene ne-galactose metabolism.
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i-beta-D-Galactose pentaacetate CAS:4163-60-4
I-Beta-D-Galactose pentaacetate iyinhlanganisela yamakhemikhali etholakala ku-galactose, ushukela we-monosaccharide.Yakhiwa nge-acetylating iqembu ngalinye le-hydroxyl le-molecule ye-galactose enamaqembu amahlanu e-acetyl.
Le nhlanganisela ivame ukusetshenziswa njenge-ejenti yokuvikela i-galactose ekuphenduleni kwamakhemikhali okuhlukahlukene kanye nezinqubo zokwenziwa.Ifomu le-pentaacetate lisiza ukuzinzisa i-galactose futhi livimbele ukusabela okungafuneki noma ukuguqulwa ngesikhathi sokusabela.
Ukwengeza, le nhlanganisela ingasetshenziswa njengesandulela sokuhlanganiswa kwezinye izinto eziphuma ku-galactose.Amaqembu e-acetyl angasuswa ngokukhetha ukuze athole okuphuma kokunye okuhlukile kwe-galactose ngamaqembu athile asebenzayo.
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5-Bromo-4-chloro-3-indolyl-beta-D-glucuronide sodium usawoti CAS:129541-41-9
I-5-Bromo-4-chloro-3-indolyl-beta-D-glucuronide usawoti we-sodium iyinhlanganisela yamakhemikhali evame ukusetshenziswa ocwaningweni lwaselabhorethri kanye nokuxilongwa.Ivamise ukubizwa nge-X-Gluc futhi isetshenziswa kabanzi njenge-substrate yokuthola umsebenzi we-beta-glucuronidase enzyme.
Uma i-beta-glucuronidase ikhona, ihlukanisa isibopho se-glucuronide ku-X-Gluc, okuholela ekukhululweni kodayi oluhlaza okwesibhakabhaka okuthiwa i-5-bromo-4-chloro-3-indolyl.Lokhu kusabela kuvame ukusetshenziselwa ukubona ngokubonakalayo noma nge-spectrophotometric ukuvezwa kwe-enzyme ye-beta-glucuronidase kumaseli noma izicubu.
Ifomu likasawoti we-sodium le-X-Gluc lithuthukisa ukunyibilika kwalo ezixazululweni ezinamanzi, kwenze kube lula ukusetshenziswa kwalo ekuhlolweni kwaselabhorethri.I-X-Gluc isetshenziswa kakhulu ocwaningweni lwebhayoloji yamangqamuzana ukutadisha isisho sofuzo, umsebenzi womgqugquzeli, kanye nokuhlolwa kofuzo lwentatheli.Ingase futhi isetshenziselwe ukuthola ubukhona bezinto eziphilayo ezikhiqiza i-beta-glucuronidase, njengamabhaktheriya athile, ezifundweni ze-microbiological.
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4-Nitrophenyl-beta-D-xylopyranoside CAS:2001-96-9
I-4-Nitrophenyl-beta-D-xylopyranoside i-substrate ye-chromogenic esetshenziswa ekuhlolweni kwe-enzymatic ukuze kutholwe futhi kukale umsebenzi wama-enzyme abizwa ngokuthi i-beta-xylosidases.
