I-Nitrotetrazolium Blue Chloride CAS:298-83-9

I-Nitrotetrazolium Blue Chloride (NBT) iyinkomba ye-redox evame ukusetshenziswa ekuhlolweni kwezinto eziphilayo kanye nezamakhemikhali. Iyimpuphu ephuzi ekhanyayo ephenduka ibe luhlaza okwesibhakabhaka uma incishisiwe, okwenza kube wusizo ekutholeni ukuba khona kwama-enzyme athile kanye nomsebenzi we-metabolic.

I-NBT isabela nabathwali bama-electron nama-enzyme afana nama-dehydrogenase, ahilelekile ezinqubweni ezahlukene zamaseli. Lapho i-NBT incishiswa yilawa ma-enzyme, yakha i-blue formazan precipitate, okuvumela ukutholwa okubonakalayo noma kwe-spectrophotometric.

Le reagent ivame ukusetshenziswa ekuhlolweni okufana nokuhlolwa kokunciphisa i-NBT, lapho isetshenziswa khona ukuhlola umsebenzi wokuqhuma kokuphefumula kwamaseli omzimba. Ingasetshenziswa futhi ukutadisha imisebenzi ye-enzyme kanye nezindlela ze-metabolic ocwaningweni oluphathelene nokucindezeleka kwe-oxidative, ukuphila kwamaseli, kanye nokuhlukaniswa kwamaseli.

I-NBT ithole izinhlelo zokusebenza emikhakheni eyahlukene, okuhlanganisa i-microbiology, i-immunology, kanye ne-cell biology. Iyakwazi ukushintshashintsha, izinzile, futhi kulula ukuyisebenzisa, okwenza kube ukukhetha okuthandwayo kwezinqubo eziningi zokuhlola.

Oda noma Cela Isilinganiso

Imininingwane Yomkhiqizo

Amathegi Omkhiqizo

Ukusetshenziswa kanye Nomphumela

Umphumela oyinhloko we-NBT ukwakheka kwe-blue formazan precipitate lapho incishiswa ama-enzyme athile. Lokhu kushintsha kombala kuvumela ukubonwa okubonakalayo noma kwe-spectrophotometric komsebenzi we-enzyme.

I-NBT inezindlela ezahlukahlukene zokusebenzisa ocwaningweni nasekuxilongeni. Nazi ezinye zezindlela eziyinhloko zokusebenzisa kwayo:

Ukuhlolwa komsebenzi we-enzyme: I-NBT ingasetshenziswa ukukala umsebenzi wama-dehydrogenase, ahilelekile ezinqubweni ezifana nokuphefumula kwamaseli kanye nokusetshenziswa kwe-metabolism. Ngokuqapha ukwehla kwe-NBT ibe yi-formazan, abacwaningi bangahlola umsebenzi wala ma-enzyme.

Ukuhlolwa komsebenzi wamaseli omzimba: I-NBT ivame ukusetshenziswa esivivinyweni sokunciphisa i-NBT ukuhlola umsebenzi wokuqhuma kokuphefumula kwamaseli omzimba, ikakhulukazi ama-phagocyte. Ukuhlolwa kulinganisa ikhono lala maseli lokukhiqiza izinhlobo ze-oxygen ezisabelayo, ezinganciphisa i-NBT futhi zakhe ukusha kwe-blue precipitate.

Ucwaningo lwe-microbiology: I-NBT isetshenziswa ku-microbiology ukutadisha i-metabolism yamagciwane nokuhlola umsebenzi wama-enzyme athile. Isibonelo, isetshenziswe ukuthola ama-bacterial nitrate reductases noma ama-bacteria enza i-formazan.

Izifundo zokuphila kwamaseli: Ukunciphisa i-NBT kuvumela abacwaningi ukuthi bahlole umsebenzi we-metabolic kanye nokuphila kwamaseli. Ngokulinganisa ubukhali bomkhiqizo we-blue formazan, kungenzeka ukuthola inani lamaseli asebenzayo kusampula ethile.

Isampula Yomkhiqizo

Ukupakisha Umkhiqizo:

Ulwazi Olwengeziwe:

Ukwakheka	C40H30ClN10O6+
Ukuhlolwa	99%
Ukubukeka	Impuphu ephuzi
Inombolo ye-CAS	298-83-9
Ukupakisha	Encane futhi enkulu
Impilo Yeshelufu	iminyaka engu-2
Isitoreji	Gcina endaweni epholile neyomile
Isitifiketi	I-ISO.