I-DDT CAS:3483-12-3 Intengo Yomkhiqizi

I-DL-Dithiothreitol, eyaziwa nangokuthi i-DTT, iyi-ejenti yokunciphisa evame ukusetshenziswa ocwaningweni lwe-biochemical kanye ne-molecular biology. Iyi-molecule encane eneqembu le-thiol (eliqukethe i-sulfur) ekugcineni ngakunye.

I-DTT ivame ukusetshenziselwa ukuphula izibopho ze-disulfide kumaprotheni, okusiza ukuwavula noma ukuwasusa. Lokhu kunciphisa izibopho ze-disulfide kubalulekile ezinqubweni ezahlukene zelebhu njengokuhlanzwa kwamaprotheni, i-gel electrophoresis, kanye nezifundo zesakhiwo samaprotheni. I-DTT ingasetshenziswa futhi ukuvikela amaqembu e-thiol nokuvimbela i-oxidation ngesikhathi sezinqubo zokuhlola.

I-DTT ivame ukungezwa ezixazululweni zokuhlola ngamanani amancane, futhi umsebenzi wayo uncike ekubeni khona komoya-mpilo. Kubalulekile ukuphatha i-DTT ngokucophelela njengoba izwela emoyeni, ekushiseni, kanye nomswakama, okunganciphisa ukusebenza kwayo.

Oda noma Cela Isilinganiso

Imininingwane Yomkhiqizo

Amathegi Omkhiqizo

Ukusetshenziswa kanye Nomphumela

Ukunciphisa Ama-Disulfide Bonds: I-DTT isetshenziswa kakhulu ukuphula ama-disulfide bonds, okuyizibopho ezihambisanayo ezakhiwe phakathi kwezinsalela ezimbili ze-cysteine kumaprotheni. Ngokunciphisa lezi zibopho, i-DTT isiza ekuguquleni amaprotheni abe ngamaprotheni, okwenza kube lula ukutadisha isakhiwo kanye nomsebenzi wawo.

Ukugoqwa Kwamaprotheni: I-DTT ingasiza ekugoqweni kahle kwamaprotheni ngokuvimbela ukwakheka okungalungile kwesibopho se-disulfide. Inciphisa noma yiziphi izibopho ze-disulfide ezingezona ezasendaweni ezingase zakheke ngesikhathi sokugoqwa kwamaprotheni, okuvumela iphrotheni ukuthi yamukele ukwakheka kwayo kwasendaweni.

Umsebenzi we-Enzyme: I-DTT ingavuselela ama-enzyme athile ngokunciphisa noma yiziphi izibopho ze-disulfide ezivimbelayo ezikhona. Ngaphezu kwalokho, i-DTT ingavimbela ukushiswa kwezinsalela ezibalulekile ze-cysteine, okungadingeka emsebenzini we-enzyme.

Ukukhiqizwa Kwama-Antibody: I-DTT ivame ukungezwa ukunciphisa ama-disulfide bonds ngesikhathi sokukhiqizwa kwama-antibodies. Kuyasiza ekuvimbeleni ukwakheka kwama-disulfide bonds angalungile, okungavimbela ukubopha kahle kwe-antigen.

Ukuqinisa Amaprotheni: I-DTT ingasetshenziswa ukuqinisa amaprotheni ngokuvimbela ukushiswa noma ukuhlangana kwawo. Kusiza ukugcina isimo esinciphile samaprotheni ngesikhathi sokugcina kanye nezinqubo zokuhlola.

Ama-Reducing Ejenti ku-Molecular Biology: I-DTT ivame ukusetshenziswa ezindleleni ezahlukene ze-molecular biology njengokulandelana kwe-DNA, i-PCR, kanye nokuhlanzwa kwamaprotheni. Ingasiza ekugcineni isimo esinciphile sezingxenye ezibalulekile, iqinisekise imiphumela emihle kakhulu yokuhlola.

Isampula Yomkhiqizo

Ukupakisha Umkhiqizo:

Ulwazi Olwengeziwe:

Ukwakheka	C4H10O2S2
Ukuhlolwa	99%
Ukubukeka	Impuphu emhlophe
Inombolo ye-CAS	3483-12-3
Ukupakisha	Encane futhi enkulu
Impilo Yeshelufu	iminyaka engu-2
Isitoreji	Gcina endaweni epholile neyomile
Isitifiketi	I-ISO.