I-ONPG CAS:369-07-3 Intengo Yomkhiqizi

I-O-nitrophenyl-β-D-galactopyranoside (ONPG) iyi-substrate yokwenziwa esetshenziswa ekuhlolweni kwe-biochemical kanye ne-molecular biology ukukala umsebenzi we-enzyme i-β-galactosidase. Ivame ukusetshenziswa ekuhlolweni ukuthola ukubonakaliswa kwezakhi zofuzo ezinhlelweni zamagciwane, njenge-Escherichia coli. I-ONPG iyi-compound engenambala ehlukaniswa yi-β-galactosidase, okuholela ekukhishweni kwe-compound ephuzi, i-o-nitrophenol. Umbala ophuzi okhiqizwayo ungalinganiswa nge-spectrophotometric, unikeze isilinganiso esingaqondile somsebenzi we-enzyme. Ukuhlolwa okusebenzisa i-ONPG kuvame ukubizwa ngokuthi i-ONPG assay futhi kuyindlela esetshenziswa kabanzi ocwaningweni lwe-molecular biology ukuhlola amazinga okubonakaliswa kwezakhi zofuzo ezilawulwa yi-lac operon kumaseli ebhaktheriya.

Oda noma Cela Isilinganiso

Imininingwane Yomkhiqizo

Amathegi Omkhiqizo

Ukusetshenziswa kanye Nomphumela

Umphumela we-ONPG njenge-substrate uzohlukaniswa yi-enzyme i-β-galactosidase, okuholela ekukhishweni komkhiqizo ophuzi, i-o-nitrophenol. Lokhu kushintsha kombala kungalinganiswa nge-spectrophotometric, okuvumela ukulinganiswa komsebenzi we-β-galactosidase. Ukusetshenziswa kwe-ONPG kugxile kakhulu ekuhlolweni kokubonakaliswa kwezakhi zofuzo ku-molecular biology kanye nocwaningo lwe-microbiology. Ngokuvamile kusetshenziselwa ukukala umsebenzi we-β-galactosidase njengentatheli yezifundo zokubonakaliswa kwezakhi zofuzo, ikakhulukazi kumabhaktheriya afana ne-E. coli. Izakhi zofuzo ze-lacZ, ezifaka ikhodi ye-β-galactosidase, zivame ukusetshenziswa njengophawu lokuhlaziywa kokubonakaliswa kwezakhi zofuzo, njengoba ukubonakaliswa kwazo kungabangelwa yizimo ezithile noma kulawulwa ngabathuthukisi abathile. Ukuhlolwa kwe-ONPG kunikeza indlela elula nethembekile yokuhlola izinga lokubonakaliswa kwezakhi zofuzo ngokulinganisa umsebenzi we-β-galactosidase. Lokhu kuhlolwa kusetshenziswa kabanzi ezinhlotsheni ezahlukene, njengokutadisha umsebenzi wokukhuthaza, ukulawulwa kwezakhi zofuzo, kanye nokusebenzisana kwamaprotheni namaprotheni. Ngaphezu kwalokho, ingasetshenziswa ukunquma i-enzyme kinetics nokuhlola imiphumela yokuguqulwa kwezakhi zofuzo noma ukwelashwa emsebenzini we-enzyme.

Isampula Yomkhiqizo

Ukupakisha Umkhiqizo:

Ulwazi Olwengeziwe:

Ukwakheka	C12H15NO8
Ukuhlolwa	99%
Ukubukeka	Impuphu emhlophe
Inombolo ye-CAS	369-07-3
Ukupakisha	Encane futhi enkulu
Impilo Yeshelufu	iminyaka engu-2
Isitoreji	Gcina endaweni epholile neyomile
Isitifiketi	I-ISO.