5-Bromo-4-chloro-3-indolyl-beta-D-glucuronide sodium salt CAS:129541-41-9 Isithombe Esifakiwe

5-Bromo-4-chloro-3-indolyl-beta-D-glucuronide usawoti wesodium I-CAS:129541-41-9

Usawoti we-sodium i-5-Bromo-4-chloro-3-indolyl-beta-D-glucuronide uyinhlanganisela yamakhemikhali evame ukusetshenziswa ocwaningweni nasekuxilongweni kwelabhorethri. Ivame ukubizwa ngokuthi i-X-Gluc futhi isetshenziswa kabanzi njengesisekelo sokuthola umsebenzi we-enzyme ye-beta-glucuronidase.

Uma i-beta-glucuronidase ikhona, ihlukanisa isibopho se-glucuronide ku-X-Gluc, okuholela ekukhululweni kwedayi eluhlaza okwesibhakabhaka ebizwa ngokuthi i-5-bromo-4-chloro-3-indolyl. Lokhu kusabela kuvame ukusetshenziselwa ukubona ngokubonakalayo noma nge-spectrophotometric ukubonakaliswa kwe-enzyme ye-beta-glucuronidase emaseli noma ezicutshini.

Uhlobo lwe-sodium salt lwe-X-Gluc luthuthukisa ukuncibilika kwalo ezixazululweni zamanzi, okwenza kube lula ukusetshenziswa kwalo ezivivinyweni zelebhu. I-X-Gluc isetshenziswa kakhulu ocwaningweni lwe-molecular biology ukuze kufundwe ukubonakaliswa kwezakhi zofuzo, umsebenzi we-promoter, kanye nokuhlolwa kwezakhi zofuzo ze-reporter. Ingasetshenziswa futhi ukuthola ukuba khona kwezinto eziphilayo ezikhiqiza i-beta-glucuronidase, njengebhaktheriya ethile, ezifundweni ze-microbiological.

Oda noma Cela Isilinganiso

Imininingwane Yomkhiqizo

Amathegi Omkhiqizo

Ukusetshenziswa kanye Nomphumela

Ukutholwa kwe-GUS: I-X-Gluc ihlukaniswa yi-enzyme ye-GUS ibe yinhlanganisela eluhlaza okwesibhakabhaka enganyibiliki eyaziwa ngokuthi i-5-bromo-4-chloro-3-indole (X-Ind). Lokhu kusabela kuvumela ukubonakala kanye nokulinganisa umsebenzi we-GUS kumaseli nezicubu.

Izifundo zokubonakaliswa kwezakhi zofuzo: I-X-Gluc isetshenziswa njenge-molecule yemibiko ezifundweni zokubonakaliswa kwezakhi zofuzo. Ngokuhlanganisa i-GUS gene nomgqugquzeli wesithakazelo, abacwaningi banganquma umsebenzi kanye nephethini yokubonakaliswa kwendawo nesikhathi yomgqugquzeli ngokubona umsebenzi we-GUS kusetshenziswa i-X-Gluc.

Ukuhlaziywa kwezitshalo eziguqulwe izakhi zofuzo: Uhlelo lwezakhi zofuzo lwe-GUS reporter lusetshenziswa kabanzi ku-biology yama-molecule ezitshalo. Ukufaka imibala ku-X-Gluc kuvumela abacwaningi ukuthi bathole futhi bafunde amaphethini okubonakaliswa kwezakhi zofuzo ezitshalweni. Lokhu kusiza ekuqondeni ukulawulwa kwezakhi zofuzo, ukubonakaliswa kwezicubu ezithile, kanye ne-biology yokuthuthukiswa kwezitshalo.

Ubunjiniyela bezakhi zofuzo: I-X-Gluc isetshenziswa njengophawu olukhethekayo ekuhlolweni kobunjiniyela bezakhi zofuzo. Ngokuxhumanisa isakhi sofuzo se-GUS nesakhi sofuzo sangaphandle esithakazelisayo, ukudaya kwe-X-Gluc kungasetshenziswa ukuhlonza ukuguqulwa okuphumelelayo kanye nokuhlanganiswa kwezakhi zofuzo ezifiselekayo emzimbeni.

Ucwaningo lwe-microbiology: I-X-Gluc ingasetshenziswa ukuthola nokuhlonza amabhaktheriya akhiqiza i-GUS. I-enzyme i-GUS itholakala ezinhlotsheni eziningi ezahlukene zamabhaktheriya, futhi ukudaya nge-X-Gluc kuvumela ukubonakala nokuhlonza amabhaktheriya ane-GUS ezifundweni ze-microbiology.

Isampula Yomkhiqizo

Ukupakisha Umkhiqizo:

Ulwazi Olwengeziwe:

Ukwakheka	C14H14BrClNNaO7
Ukuhlolwa	99%
Ukubukeka	Impuphu emhlophe
Inombolo ye-CAS	129541-41-9
Ukupakisha	Encane futhi enkulu
Impilo Yeshelufu	iminyaka engu-2
Isitoreji	Gcina endaweni epholile neyomile
Isitifiketi	I-ISO.