-
I-TAPSO CAS: 68399-81-5 Intengo Yomkhiqizi
I-TAPSO (3-[N-tris(hydroxymethyl)methyl]amino]-2-hydroxypropanesulfonic acid) iyi-zwitterionic buffer evame ukusetshenziswa ocwaningweni lwezinto eziphilayo kanye nezamakhemikhali. Iyi-ejenti yokuvimba esebenza kahle ene-pKa eseduze ne-pH yezinto eziphilayo, okwenza ifaneleke ukugcina i-pH ezinzile ekuhlolweni kwezinto eziphilayo. I-TAPSO ivame ukusetshenziswa ekuhlanzweni kwamaprotheni, ekuhlolweni kwama-enzyme, ekukhuleni kwamaseli, kanye nasekusetshenzisweni kwe-electrophoresis. Ukuncibilika kwayo emanzini amaningi kanye nokuphazamiseka okuphansi kwezinqubo zezinto eziphilayo kwenza kube ukukhetha okuthandwayo emphakathini wesayensi. I-TAPSO yaziwa ngomphumela wayo omncane emsebenzini wama-enzyme futhi ivame ukusetshenziswa njengenye indlela kwezinye izinto zokuvimba ezifana nama-Tris noma ama-phosphate buffers.
-
I-6-Methyl-2-pyridinyl2-(acetylamino)-2-deoxy-1-thio-beta-D-glucopyranoside CAS:149263-94-5
I-6-Methyl-2-pyridinyl 2-(acetylamino)-2-deoxy-1-thio-beta-D-glucopyranoside, eyaziwa nangokuthi i-N-acetylglucosamine thiolactone, iyinhlanganisela yamakhemikhali enesakhiwo esikhethekile equkethe indandatho ye-pyridine, iqembu le-acetylamino, kanye nengxenye ye-thiolactone.
.
-
I-Sodium Glycocholate CAS: 863-57-0
I-Sodium glycocholate iyinhlanganisela kasawoti we-bile esiza ekugayweni nasekumuncweni kwamafutha ekudleni. Yenziwa nge-glycine kanye ne-cholic acid futhi idlala indima ebalulekile ekuhlukaniseni amafutha abe amaconsi amancane, yandisa indawo yawo engaphezulu ukuze kugaywe kahle. I-Sodium glycocholate incibilika kakhulu emanzini futhi isetshenziswa ekwakhiweni kwemithi ukuthuthukisa ukuncibilika kanye nokutholakala kwemithi engancibiliki kahle. Ngaphezu kwalokho, isetshenziswa ocwaningweni nasezindaweni zelebhu ukusekela ukukhula kwamagciwane athile kanye nezinhlobo zamaseli..
-
I-TAPS Usawoti we-Sodium I-CAS: 70331-82-7
Usawoti we-TAPS sodium, owaziwa nangokuthi i-N-(Tris(hydroxymethyl)methyl)-2-aminoethanesulfonic acid sodium salt, uyi-ejenti yokubopha eguquguqukayo kanye nomlawuli we-pH. Ivame ukusetshenziswa ocwaningweni lwezinto eziphilayo kanye namakhemikhali, kwezemithi, nasekuxilongeni. I-TAPS-Na iyinhlanganisela ye-zwitterionic esiza ukugcina i-pH ezinzile ezixazululweni, okwenza ifaneleke ezimweni eziningi zokuhlola. Ukusetshenziswa kwayo kufaka phakathi isiko lamaseli, ucwaningo lwamaprotheni, i-electrophoresis, ukuhlanganiswa kwamakhemikhali, kanye nokwakheka kwemithi. I-TAPS-Na iwuhlobo lwe-TAPS oluncibilikayo noluzinzile okulula ukuluphatha nokusebenzisa ezixazululweni zamanzi.
-
I-NBD chloride CAS: 10199-89-0
I-NBD chloride, eyaziwa nangokuthi i-7-chloro-4-nitrobenzo-2-oxa-1,3-diazole, iwudayi we-fluorescent ovame ukusetshenziswa ekusetshenzisweni kwamakhemikhali kanye ne-biological. Ingaphansi kwesigaba se-nitrobenzoxadiazole (NBD), esibonisa izakhiwo eziqinile ze-fluorescence.
I-NBD chloride ibonakala ngekhono layo lokwakha izibopho ze-covalent nama-nucleophile, njenge-primary amines, ama-thiols, namaqembu e-hydroxyl. Lesi sici siyivumela ukuthi isetshenziswe njenge-ejenti yokulebula ama-biomolecule, okuhlanganisa amaprotheni, ama-peptides, ama-nucleic acid, namanye ama-molecule amancane.
Uma i-NBD chloride isabela ne-nucleophile, ibhekana nokusabela kokushintsha, okuholela ekwakhekeni komkhiqizo oqinile onelebula le-NBD. Lo mkhiqizo onelebula ubonisa ukukhishwa okukhulu kwe-fluorescence, okwenza kube usizo ezindleleni ezahlukene zokuthola nokuthwebula izithombe.
Ukukhishwa kwe-fluorescence kwama-molecule anelebula le-NBD kungavuselelwa ngokukhanya kwe-ultraviolet (UV) cishe ku-470 nm, futhi ukukhanya okukhishwe ngokuvamile kutholakala cishe ku-530 nm. Lokhu kuphakama kokukhanya kuwela esifundeni esiluhlaza se-spectrum ebonakalayo, lapho ukuphazamiseka kokutholwa okuvela kumasampula ebhayoloji kuphansi kakhulu.
-
I-Proteinase K CAS:39450-01-6 Intengo Yomkhiqizi
I-Proteinase K iyi-enzyme ye-serine protease esebenza kakhulu engakwazi ukuvuselela i-hydrolysis yezibopho ze-peptide kumaprotheni. Itholakala ku-albhamu ye-fungus i-Engyodontium futhi yaziwa ngobubanzi bayo bokucaciswa kwe-substrate. I-Proteinase K ingahlukanisa ngempumelelo izibopho ze-peptide ohlangothini lwe-C lwe-hydrophobic, aliphatic, noma ama-aromatic amino acid, okwenza ibe usizo ezinhlelweni ezahlukahlukene ze-biology yama-molecule kanye ne-biochemistry..
-
I-N,N-Dimethyl-p-phenylenediamine dihydrochloride CAS:536-46-9
I-N,N-Dimethyl-p-phenylenediamine dihydrochloride iyinhlanganisela yamakhemikhali evame ukusetshenziswa ezinhlotsheni ezahlukene zesayensi nezimboni. Iyimpuphu yekristalu emhlophe kuya kwemhlophe enefomula yama-molecule engu-C8H14N2·2HCl.
Le nhlanganisela inokusetshenziswa okubanzi ngenxa yezakhiwo zayo kanye nokusabela kwayo. Ivame ukusetshenziswa njenge-reagent yokuhlaziya yokunquma ama-peroxide kanye nodayi ophakathi ekukhiqizweni kwezinto ezinemibala ehlukahlukene kanye nemibala.
Emkhakheni we-analytical chemistry, i-N,N-Dimethyl-p-phenylenediamine dihydrochloride ivame ukusetshenziswa njengesibonakaliso se-colorimetric sokunquma izinhlobo ze-oxidative, njenge-hydrogen peroxide noma ama-peroxide kumasampula. I-compound idlula ekushintsheni kombala lapho isabela nalezi zidakamizwa ezi-oxidizing, okuvumela ukulinganiswa kwazo.
Ngaphezu kwalokho, le nhlanganisela ingatholakala nasekwakheni amadayi ezinwele, lapho isebenza khona njengonjiniyela wombala. Amandla ayo okudala imibala egqamile nehlala isikhathi eside ayenza ithandwe embonini yezimonyo.
-
Usawoti we-Creatine phosphate disodium CAS:922-32-7
Usawoti we-disodium we-Creatine phosphate uyinhlanganisela equkethe i-creatine, i-amino acid derivative eyenzeka ngokwemvelo, eboshwe emaqenjini e-phosphate kanye nama-ion e-sodium. Idlala indima ebalulekile ekuhlinzekeni amandla okunciphisa imisipha futhi ivame ukusetshenziswa njengesengezo sokudla ngabasubathi nabakhi bomzimba.
Uma isetshenziswa, usawoti we-disodium we-creatine phosphate uguqulwa ube yi-creatine phosphate emzimbeni, bese ugcinwa emisipheni. Ngesikhathi semisebenzi enamandla kakhulu, njengokuphakamisa izinsimbi noma ukusheshisa, i-creatine phosphate isebenza njengomthombo otholakala kalula wezibopho ze-phosphate ezinamandla aphezulu, okuvumela ukugcwaliswa kabusha okusheshayo kwe-adenosine triphosphate (ATP).
I-ATP iyi-molecule yamandla eyinhloko esetshenziswa yimisipha, futhi amazinga ayo ancipha ngokushesha ngesikhathi sokuzivocavoca okunamandla. Ngokunezela nge-creatine phosphate, abasubathi bangandisa ukutholakala kwe-ATP, okuholela ekusebenzeni kahle kwemisipha, amandla akhuphukile, kanye nokukhathala okuncishisiwe.
Ngaphezu kwalokho, usawoti we-creatine phosphate disodium ungaba nezinye izinzuzo ezingaba khona, okuhlanganisa ukwanda kwemisipha, ukululama okuthuthukisiwe, kanye nokusebenza kwengqondo okuthuthukisiwe. Ngokuvamile uthathwa ngomlomo ngendlela yempuphu noma yethebhulethi, kungaba njengesengezo esizimele noma njengengxenye yohlelo lokwengeza ngaphambi kokuzivocavoca noma ngemuva kokuzivocavoca.
-
2′,6′-DiMethylcarbonylphenyl-10-sulfopropylacridiniuM-9-carboxylate 4′-NHS Ester CAS:194357-64-7
I-2′,6′-DiMethylcarbonylphenyl-10-sulfopropylacridinium-9-carboxylate I-4′-NHS ester iyinhlanganisela yamakhemikhali enesakhiwo sama-molecule esiyinkimbinkimbi. Iqukethe iqembu le-sulfopropylacridinium kanye neqembu elisebenzayo le-carboxylate ester. Ukuba khona kwengxenye ye-ester kubonisa ukuthi iyasabela futhi ingasetshenziswa njenge-ejenti yokulebula noma yokuguqula ama-biomolecule.
Iqembu le-sulfopropylacridinium lale nhlanganisela lisikisela ukuthi lingase libe nokusetshenziswa okungenzeka ekuhlolweni okusekelwe ku-fluorescence, lapho lingasetshenziswa khona njenge-probe noma idayi ye-fluorescent yokuthola nokuhlaziya ama-biomolecule. Lingase futhi libe nokubaluleka ezifundweni zezinqubo zamaseli, njengokubonisana kwe-calcium ngaphakathi kweseli.
Ukufakwa kweqembu le-NHS ester kubonisa ukuthi lingasabela nama-amine ayinhloko, njengalawo atholakala kumaprotheni noma kuma-peptide, ukuze kwakheke ama-amide bond azinzile. Lokhu kusebenza kuyenza ibe usizo ezinhlelweni zokuhlanganisa izinto eziphilayo, lapho ingasetshenziswa khona ukulebula noma ukuguqula ama-biomolecule ngamanye ama-molecule asebenzayo, njenge-fluorophores noma ama-tag.
-
I-ABEI CAS:66612-29-1 Intengo Yomkhiqizi
I-N-(4-Aminobutyl)-N-ethylisoluminol, eyaziwa nangokuthi i-ABEI, iyinhlanganisela ekhanyayo evame ukusetshenziswa njenge-probe ye-chemiluminescent ekusetshenzisweni kwe-bioanalytical. Iyingxenye yesigaba se-isoluminol derivatives futhi isetshenziswa kabanzi ekuhlolweni kwe-immunoassays kanye namasu okuthola i-DNA/RNA.
.
-
I-Z-GLY-PRO-PNA CAS: 65022-15-3
I-Z-GLY-PRO-PNA iyi-peptide nucleic acid (PNA) etholakala ku-Z-Gly-Pro sequence. Le nhlanganisela ivame ukusetshenziswa ocwaningweni nasekuxilongweni ngenxa yekhono layo lokubopha ngqo ku-nucleic acid sequences ehambisanayo. Ukwengezwa kweqembu le-Z ku-N-terminus kuvumela ukuqondiswa okuphumelelayo nokulethwa ku-molecule target oyifunayo. Ngenhlanganisela yayo eyingqayizivele ye-peptide kanye ne-nucleic acid properties, i-Z-GLY-PRO-PNA inikeza amandla amakhulu ekusetshenzisweni okuhlukahlukene, okuhlanganisa ukwelashwa kwezakhi zofuzo, ukuxilongwa kwama-molecular, kanye nokuthuthukiswa kwemithi..
-
I-TRIS-Acetate CAS: 6850-28-8 Intengo Yomkhiqizi
I-TRIS-Acetate, iyi-buffer evame ukusetshenziswa ekuhlolweni kwezinto eziphilayo kanye namakhemikhali. Iyinhlanganisela ye-Tris base kanye ne-acetic acid, okuholela esixazululweni esizinzile se-pH esisetshenziswa ukulawula nokugcina ububanzi be-pH obufunwayo bezinhlelo zokusebenza ezahlukahlukene. I-TRIS-Acetate iwusizo kakhulu ezifundweni ze-DNA kanye ne-RNA, njengoba inikeza indawo efanelekile yemisebenzi ye-enzyme, i-electrophoresis, kanye ne-gel electrophoresis. Isiza ukugcina ukuzinza kanye nobuqotho bama-nucleic acid ngesikhathi sezinqubo ezahlukahlukene, njengokulandelana kwe-DNA, i-polymerase chain reaction (PCR), kanye ne-agarose gel electrophoresis. Ngaphezu kocwaningo lwe-nucleic acid, i-TRIS-Acetate isetshenziswa futhi ezinqubweni zokuhlukanisa amaprotheni kanye nokuhlanza, ukukhipha amaprotheni e-membrane, kanye nokuhlolwa kokukhula kwamaseli. Amandla ayo okuhlanganisa aguquguqukayo ayenza ithuluzi eliwusizo ezindaweni eziningi zocwaningo lwesayensi, ukuqinisekisa izimo ezifanele zokusabela kwezinto eziphilayo kanye nokugcina ukuzinza kwama-enzyme namaprotheni.
