I-TRIS-Acetate CAS: 6850-28-8 Intengo Yomkhiqizi

I-TRIS-Acetate, iyi-buffer evame ukusetshenziswa ekuhlolweni kwezinto eziphilayo kanye namakhemikhali. Iyinhlanganisela ye-Tris base kanye ne-acetic acid, okuholela esixazululweni esizinzile se-pH esisetshenziswa ukulawula nokugcina ububanzi be-pH obufunwayo bezinhlelo zokusebenza ezahlukahlukene. I-TRIS-Acetate iwusizo kakhulu ezifundweni ze-DNA kanye ne-RNA, njengoba inikeza indawo efanelekile yemisebenzi ye-enzyme, i-electrophoresis, kanye ne-gel electrophoresis. Isiza ukugcina ukuzinza kanye nobuqotho bama-nucleic acid ngesikhathi sezinqubo ezahlukahlukene, njengokulandelana kwe-DNA, i-polymerase chain reaction (PCR), kanye ne-agarose gel electrophoresis. Ngaphezu kocwaningo lwe-nucleic acid, i-TRIS-Acetate isetshenziswa futhi ezinqubweni zokuhlukanisa amaprotheni kanye nokuhlanza, ukukhipha amaprotheni e-membrane, kanye nokuhlolwa kokukhula kwamaseli. Amandla ayo okuhlanganisa aguquguqukayo ayenza ithuluzi eliwusizo ezindaweni eziningi zocwaningo lwesayensi, ukuqinisekisa izimo ezifanele zokusabela kwezinto eziphilayo kanye nokugcina ukuzinza kwama-enzyme namaprotheni.

Oda noma Cela Isilinganiso

Imininingwane Yomkhiqizo

Amathegi Omkhiqizo

Ukusetshenziswa kanye Nomphumela

I-Tris-acetate (TRIS-Acetate) iyi-buffer evame ukusetshenziswa ekuhlolweni kwezinto eziphilayo kanye nezamakhemikhali. Iqukethe inhlanganisela ye-tris(hydroxymethyl)aminomethane (Tris) kanye ne-acetic acid, esebenza njengomlawuli we-pH kanye ne-stabilizer. I-pH ye-buffer ye-TRIS-Acetate ivame ukuba phakathi kuka-7.4 no-8.4.
Umphumela oyinhloko we-TRIS-Acetate ukugcina i-pH ezinzile, ebalulekile ekuphenduleni okuningi kwezinto eziphilayo kanye nezamakhemikhali. Isebenza njengesivikelo ngokunciphisa noma yiziphi izinguquko ezibalulekile ku-pH ezingase zenzeke ngenxa yama-asidi noma izisekelo ezengeziwe ngesikhathi sezinqubo zokuhlola.
I-TRIS-Acetate ithola ukusetshenziswa okuhlukahlukene ku-biology yamangqamuzana, i-biochemistry, kanye ne-biotechnology:
I-DNA kanye ne-RNA Electrophoresis: I-TRIS-Acetate ivame ukusetshenziswa njenge-buffer egijimayo ku-agarose kanye ne-polyacrylamide gel electrophoresis. Ihlinzeka ngendawo ye-pH ezinzile ngesikhathi sokuhlukaniswa kwezingcezu ze-DNA kanye ne-RNA ngokusekelwe kusayizi wazo.
Ukuhlaziywa Kwamaprotheni: Ama-buffer e-TRIS-Acetate asetshenziselwa i-protein electrophoresis, njenge-SDS-PAGE (Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis). Iqinisekisa ukuqina nokuhlukaniswa kwamaprotheni phakathi nenqubo.
Ukusabela Kwe-Enzyme: Ama-buffer e-TRIS-Acetate avame ukusetshenziswa ekuhlolweni nasekufundweni kwe-enzyme. Ahlinzeka ngebanga le-pH elifanele kakhulu lemiphumela ehlukahlukene ye-enzyme futhi asize ekugcineni umsebenzi we-enzyme.
Ukukhuliswa Kwamaseli Nezicubu: Ama-buffer e-TRIS-Acetate asetshenziswa kwimidiya yokukhulisa amaseli ukugcina i-pH efanele yokukhula nokwanda kwamaseli. Kuyasiza ukugcina izimo zomzimba ezidingekayo ukuze amaseli aphile kahle.

Ukupakisha Umkhiqizo:

Ulwazi Olwengeziwe:

Ukwakheka	C6H15NO5
Ukuhlolwa	99%
Ukubukeka	Impuphu emhlophe
Inombolo ye-CAS	6850-28-8
Ukupakisha	Encane futhi enkulu
Impilo Yeshelufu	iminyaka engu-2
Isitoreji	Gcina endaweni epholile neyomile
Isitifiketi	I-ISO.