I-NBD chloride CAS: 10199-89-0

I-NBD chloride, eyaziwa nangokuthi i-7-chloro-4-nitrobenzo-2-oxa-1,3-diazole, iwudayi we-fluorescent ovame ukusetshenziswa ekusetshenzisweni kwamakhemikhali kanye ne-biological. Ingaphansi kwesigaba se-nitrobenzoxadiazole (NBD), esibonisa izakhiwo eziqinile ze-fluorescence.

I-NBD chloride ibonakala ngekhono layo lokwakha izibopho ze-covalent nama-nucleophile, njenge-primary amines, ama-thiols, namaqembu e-hydroxyl. Lesi sici siyivumela ukuthi isetshenziswe njenge-ejenti yokulebula ama-biomolecule, okuhlanganisa amaprotheni, ama-peptides, ama-nucleic acid, namanye ama-molecule amancane.

Uma i-NBD chloride isabela ne-nucleophile, ibhekana nokusabela kokushintsha, okuholela ekwakhekeni komkhiqizo oqinile onelebula le-NBD. Lo mkhiqizo onelebula ubonisa ukukhishwa okukhulu kwe-fluorescence, okwenza kube usizo ezindleleni ezahlukene zokuthola nokuthwebula izithombe.

Ukukhishwa kwe-fluorescence kwama-molecule anelebula le-NBD kungavuselelwa ngokukhanya kwe-ultraviolet (UV) cishe ku-470 nm, futhi ukukhanya okukhishwe ngokuvamile kutholakala cishe ku-530 nm. Lokhu kuphakama kokukhanya kuwela esifundeni esiluhlaza se-spectrum ebonakalayo, lapho ukuphazamiseka kokutholwa okuvela kumasampula ebhayoloji kuphansi kakhulu.

Oda noma Cela Isilinganiso

Imininingwane Yomkhiqizo

Amathegi Omkhiqizo

Ukusetshenziswa kanye Nomphumela

Ukulebula kwe-Covalent: I-NBD chloride ingakha izibopho ze-covalent namaqembu e-nucleophilic kuma-biomolecule, njenge-primary amines, ama-thiols, namaqembu e-hydroxyl. Lokhu kuvumela ukulebula okukhethekile kwala ma-biomolecule ngezinjongo zokuthola nokuthwebula izithombe.

Ukuthuthukiswa kwe-Fluorescence: Lapho i-NBD chloride isabela nama-nucleophiles, yakha umkhiqizo onelebula le-NBD elikhanyayo. Lo mkhiqizo onelebula ubonisa ukukhishwa okunamandla kwe-fluorescence, okwenza kube usizo ekuboniseni nasekulandeleleni ama-molecule anelebula.

Ukuzwela kanye nokucaciswa: Ukukhishwa kwe-fluorescence kwemikhiqizo enelebula le-NBD kubucayi kakhulu, okuvumela ukutholakala kwamanani aphansi ama-molecule anelebula. Ngaphezu kwalokho, ukwakheka kwesibopho se-covalent phakathi kwe-NBD chloride nama-nucleophiles kunikeza ukucaciswa, njengoba kuqinisekisa ukunamathela okuzinzile nokuqondile kwedayi ku-biomolecule eqondiwe.

Ukulebula kwe-biomolecule: I-NBD chloride ingasetshenziswa ukulebula ama-biomolecule ahlukahlukene, okuhlanganisa amaprotheni, ama-peptide, ama-nucleic acid, namanye ama-molecule amancane. Lokhu kwenza abacwaningi bakwazi ukuphenya indawo, amandla, kanye nokusebenzisana kwala ma-biomolecule ezinhlelweni zezinto eziphilayo.

I-Fluorescence microscopy: Ama-biomolecule anelebula le-NBD angabonakala kusetshenziswa i-fluorescence microscopy. Le ndlela ivumela ukubonwa kwama-molecule anelebula ezingeni leseli noma le-subcellular, okunikeza ulwazi olubalulekile mayelana nokusatshalaliswa kwawo kanye nokuziphatha kwawo.

I-Flow cytometry: Ama-biomolecule anelebula le-NBD angahlaziywa futhi alinganiswe kusetshenziswa i-flow cytometry. Le ndlela ivumela ukuhlaziywa okuphezulu kwamaseli noma izinhlayiya ezinelebula, okunikeza ulwazi mayelana namazinga okuvezwa noma izakhiwo zobuso zama-biomolecule anelebula.

Ukulebula nokulandelela amaprotheni: I-NBD chloride ingasetshenziswa ukulebula izinsalela ezithile ze-amino acid noma amaqembu asebenzayo kumaprotheni. Lokhu kwenza abacwaningi bakwazi ukutadisha isakhiwo samaprotheni, ukugoqa, amandla, kanye nokusebenzisana ngesikhathi sangempela.

Ukupakisha Umkhiqizo:

Ulwazi Olwengeziwe:

Ukwakheka	C6H2ClN3O3
Ukuhlolwa	99%
Ukubukeka	Impuphu ephuzi
Inombolo ye-CAS	10199-89-0
Ukupakisha	Encane futhi enkulu
Impilo Yeshelufu	iminyaka engu-2
Isitoreji	Gcina endaweni epholile neyomile
Isitifiketi	I-ISO.