I-TAPSO CAS: 68399-81-5 Intengo Yomkhiqizi

I-TAPSO (3-[N-tris(hydroxymethyl)methyl]amino]-2-hydroxypropanesulfonic acid) iyi-zwitterionic buffer evame ukusetshenziswa ocwaningweni lwezinto eziphilayo kanye nezamakhemikhali. Iyi-ejenti yokuvimba esebenza kahle ene-pKa eseduze ne-pH yezinto eziphilayo, okwenza ifaneleke ukugcina i-pH ezinzile ekuhlolweni kwezinto eziphilayo. I-TAPSO ivame ukusetshenziswa ekuhlanzweni kwamaprotheni, ekuhlolweni kwama-enzyme, ekukhuleni kwamaseli, kanye nasekusetshenzisweni kwe-electrophoresis. Ukuncibilika kwayo emanzini amaningi kanye nokuphazamiseka okuphansi kwezinqubo zezinto eziphilayo kwenza kube ukukhetha okuthandwayo emphakathini wesayensi. I-TAPSO yaziwa ngomphumela wayo omncane emsebenzini wama-enzyme futhi ivame ukusetshenziswa njengenye indlela kwezinye izinto zokuvimba ezifana nama-Tris noma ama-phosphate buffers.

Oda noma Cela Isilinganiso

Imininingwane Yomkhiqizo

Amathegi Omkhiqizo

Ukusetshenziswa kanye Nomphumela

Ukuhlanzwa kwamaprotheni: I-TAPSO ivame ukusetshenziswa njenge-buffer kumasu okuhlanza amaprotheni njenge-ion exchange chromatography kanye ne-size exclusion chromatography. Amandla ayo okugcina i-buffer asiza ukugcina i-pH efiselekayo kuyo yonke inqubo yokuhlanza, okuqinisekisa ukuqina kwamaprotheni.

Ukuhlolwa kwe-enzyme: I-TAPSO isetshenziswa ekuhlolweni komsebenzi we-enzyme ukuze inikeze indawo ye-pH ehambisanayo elingisa izimo zomzimba. Ngokugcina i-pH ezinzile, i-TAPSO isiza ukuqinisekisa ukulinganiswa komsebenzi we-enzyme okunembile nokuthembekile.

Ukukhuliswa kwamaseli: I-TAPSO ivame ukusetshenziswa njengesivikelo sokugcina imidiya yokukhuliswa kwamaseli ku-pH ezinzile. Imvelo yayo ye-zwitterionic inciphisa ukusebenzisana namaseli futhi inciphise imiphumela engaba khona ye-cytotoxic engavela ngokusebenzisa ezinye izinto ezikhuphulayo.

I-Electrophoresis: I-TAPSO ingasetshenziswa njenge-buffer egijimayo kumasu e-electrophoretic, njenge-protein gel electrophoresis (SDS-PAGE) noma i-capillary electrophoresis. Amandla ayo okugcina i-buffer asiza ukugcina i-pH efiselekayo ngesikhathi senqubo yokuhlukanisa.

Ukupakisha Umkhiqizo:

Ulwazi Olwengeziwe:

Ukwakheka	C6H14NNaO4
Ukuhlolwa	99%
Ukubukeka	Impuphu emhlophe
Inombolo ye-CAS	68399-81-5
Ukupakisha	Encane futhi enkulu
Impilo Yeshelufu	iminyaka engu-2
Isitoreji	Gcina endaweni epholile neyomile
Isitifiketi	I-ISO.