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I-Heppso sodium CAS: 89648-37-3 Intengo Yomkhiqizi
Usawoti we-sodium i-N-[2-Hydroxyethyl]piperazine-N'-[2-hydroxypropanesulfonic acid] uyinhlanganisela yamakhemikhali enefomula i-C8H19N2NaO4S. Usawoti we-sodium otholakala ku-piperazine, oqukethe amaqembu asebenzayo e-hydroxyethyl kanye ne-hydroxypropanesulfonic acid. Uvame ukusetshenziswa embonini yezemithi njenge-ejenti yokubopha kanye ne-stabilizer ekwakhiweni kwemithi. Le nhlanganisela isiza ukugcina i-pH kanye nokuzinza kwemithi.
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I-P-NITROPHENYL BETA-D-LACTOPYRANOSIDE CAS:4419-94-7
I-P-Nitrophenyl beta-D-lactopyranoside, eyaziwa nangokuthi i-PNPG, iyinhlanganisela evame ukusetshenziswa ekuhlolweni kwe-enzyme ukukala umsebenzi we-beta-galactosidase, i-enzyme ehilelekile ekusetshenzisweni kwe-carbohydrate. I-PNPG iyi-substrate yokwenziwa engahlukaniswa yi-beta-galactosidase, okuholela ekukhishweni komkhiqizo ophuzi. Ubukhulu be-substrate hydrolysis bungalinganiswa nge-spectrophotometric ngokulinganisa ukumuncwa komkhiqizo ku-wavelength ethile. Lokhu kuvumela abacwaningi ukuthi bahlole umsebenzi kanye ne-kinetics ye-beta-galactosidase ezimweni ezahlukahlukene, njengokufunda umsebenzi we-enzyme, ukuhlola ama-enzyme inhibitors noma ama-activator, noma ukuhlola imiphumela yokuguqulwa kwezakhi zofuzo emsebenzini we-enzyme.
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popso sesquisodium CAS:108321-08-0
Usawoti we-Piperazine-N,N'-bis(2-hydroxypropanesulfonic acid) sesquisodium, owaziwa nangokuthi usawoti we-PIPES sesquisodium, uyinhlanganisela yamakhemikhali esetshenziswa njenge-ejenti yokubopha ezinhlotsheni ezahlukahlukene zesayensi nezimboni. Isiza ukugcina izinga le-pH elizinzile ezixazululweni, ikakhulukazi ebangeni le-pH yomzimba. Usawoti we-PIPES sesquisodium uvame ukusetshenziswa kwimidiya yokukhulisa amaseli, i-protein biochemistry, i-electrophoresis, amasu e-molecular biology, kanye nezinhlelo zokulethwa kwezidakamizwa. Isebenza njengomlawuli we-pH kanye nesithuthukisi somsebenzi we-enzyme kanye nokuzinza, okwenza kube wusizo ocwaningweni olubanzi kanye nezinqubo zezimboni.
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I-ACES CAS: 7365-82-4 Intengo Yomkhiqizi
I-N-(2-Acetamido)-2-aminoethanesulfonic acid, eyaziwa nangokuthi i-ACES, iyi-ejenti yokubopha esetshenziswa kakhulu ocwaningweni lwesayensi. Isiza ukugcina i-pH ezinzile ezixazululweni futhi incibilika kakhulu emanzini. I-ACES inobuthi obuphansi futhi ayiphazamisi kakhulu izinqubo ze-biochemical, okwenza ifaneleke ukusetshenziswa okuhlukahlukene. Ivame ukusetshenziswa njenge-buffer ezifundweni zokuzinza kwamaprotheni kanye nokuhlolwa kwama-enzyme, lapho igcina khona izimo ezifanele zokwakheka kwamaprotheni kanye nomsebenzi wama-enzyme.
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I-Phenylgalactoside CAS:2818-58-8
I-Phenylgalactoside, eyaziwa nangokuthi i-p-nitrophenyl β-D-galactopyranoside (pNPG), iyi-substrate yokwenziwa evame ukusetshenziswa ekuhlolweni kwe-biochemical kanye ne-molecular biology. Ivame ukusetshenziswa ukuthola nokulinganisa umsebenzi we-enzyme i-β-galactosidase.
Uma i-phenylgalactoside i-hydrolyzed yi-β-galactosidase, ikhipha i-p-nitrophenol, okuyinhlanganisela enombala ophuzi. Ukukhululwa kwe-p-nitrophenol kungalinganiswa ngobuningi kusetshenziswa i-spectrophotometer, njengoba ukumuncwa kwe-p-nitrophenol kungatholakala ku-wavelength engu-405 nm.
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I-DIPSO CAS: 68399-80-4 Intengo Yomkhiqizi
I-DIPSO imele i-“Diisopropyl azodicarboxylate,” okuyi-reagent evame ukusetshenziswa kumakhemikhali e-organic. Isetshenziswa kakhulu ekusabeleni kwe-Mitsunobu, okuyindlela yokuguqula ama-alcohol abe amaqembu ahlukahlukene asebenzayo njenge-esters, ethers, noma ama-amines. I-DIPSO isebenza njengomthombo we-intermediate esabela kakhulu ebizwa ngokuthi i-azodicarboxylate, elekelela lolu shintsho.
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I-4-Nitrophenyl-beta-D-glucopyranoside CAS:2492-87-7
I-4-Nitrophenyl-beta-D-glucopyranoside iyi-substrate evame ukusetshenziswa ekuhlolweni kwamakhemikhali ukuhlola umsebenzi wama-enzyme afana ne-β-glucuronidase. Le nhlanganisela i-hydrolyzed yi-enzyme, okuholela ekukhishweni kwe-4-nitrophenol, engalinganiswa kusetshenziswa i-spectrophotometry. Ukusetshenziswa kwayo kwenza abacwaningi bakwazi ukutadisha izici ezahlukahlukene ze-metabolism yezidakamizwa, ubuthi, kanye nokuxilongwa kwemitholampilo okuhlobene nokusabela kwe-glucuronidation.
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I-Tris maleate CAS:72200-76-1
I-Tris maleate iyinhlanganisela yamakhemikhali esebenza njenge-pH buffer kanye ne-adjuster ezimbonini ezahlukahlukene. Isetshenziselwa ukugcina amazinga e-pH azinzile kanye nokumelana nezinguquko ezibangelwa ukwengezwa kwama-acid noma izisekelo. I-Tris maleate ivame ukusetshenziswa ocwaningweni lwe-biochemical, ukuhlanzwa kwamaprotheni, izinqubo zezimboni, kanye ne-analytical chemistry. Isebenza kahle kakhulu ekubambeni amazinga e-pH aphansi futhi yaziwa ngokuguquguquka kwayo ekugcineni izimo ze-pH ezinhle kakhulu.
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I-MOBS CAS:115724-21-5 Intengo Yomkhiqizi
I-4-morpholin-4-ylbutane-1-sulfonic acid, eyaziwa nangokuthi i-MOBI-S, iyinhlanganisela yezinto eziphilayo ezenziwe ngokwenziwa esetshenziswa njenge-ejenti yokubopha ocwaningweni lwezinto eziphilayo kanye nezamakhemikhali. Izinzile futhi ingagcina i-pH ezinzile ebangeni elingathathi hlangothi kuya kwele-alkaline kancane.BI-S isetshenziswa kabanzi ekukhuleni kwamaseli, ekuhlolweni kwama-enzyme, kumasu e-molecular biology, kanye nase-electrophoresis. Ihambisana nezinhlobo ezahlukene zama-reagent futhi yaziwa ngokuqina kwayo kanye nokumelana nezinguquko ekushiseni kanye ne-pH.
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1,2,3,4,6-penta-O-acetyl-alpha-D-galactopyranose CAS:4163-59-1
I-1,2,3,4,6-penta-O-acetyl-alpha-D-galactopyranose iyinhlanganisela yamakhemikhali eyingxenye yomndeni wama-carbohydrate. Ivela ku-alpha-D-galactopyranose, ushukela ovela ngokwemvelo. Le nhlanganisela ethile inamaqembu amahlanu e-acetyl anamathele kumaqembu athile e-hydroxyl ku-molecule kashukela. Ivame ukusetshenziswa ezinhlotsheni ezahlukahlukene zamakhemikhali nezemithi, kufaka phakathi njengento yokuqala yokwenziwa kwamanye amakhemikhali. Uhlobo lwayo lwe-acetylated luthuthukisa ukuzinza kwayo kanye nokusebenza kwayo, okwenza kube yisisekelo esiwusizo kumakhemikhali e-organic.
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I-POPSO CAS: 68189-43-5 Intengo Yomkhiqizi
I-POPSO, isifinyezo se-Piperazine-N,N'-bis(2-hydroxypropanesulfonic acid) sesquisodium salt, iyi-agent yokubopha evame ukusetshenziswa ocwaningweni lwezinto eziphilayo kanye nezamakhemikhali. Isiza ukugcina izinga le-pH elizinzile ezixazululweni, ikakhulukazi ngaphakathi kobubanzi be-pH yezinto eziphilayo. Usawoti we-PIPES sesquisodium usetshenziswa ekukhuleni kwamaseli, i-protein biochemistry, i-electrophoresis, amasu e-molecular biology, izinhlelo zokulethwa kwemithi, nokuningi. Amandla ayo okulawula i-pH ayenza ithuluzi eliwusizo ocwaningweni oluhlukahlukene kanye nezicelo zezimboni.
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I-BES CAS: 10191-18-1 Intengo Yomkhiqizi
I-N,N-Bis(hydroxyethyl)-2-aminoethanesulfonic acid, eyaziwa nangokuthi i-BES noma i-N,N-Bis(2-hydroxyethyl)aminoethanesulfonic acid, iyinhlanganisela yamakhemikhali evame ukusetshenziswa njenge-ejenti yokubopha ezinhlotsheni ezahlukahlukene zesayensi nezimboni.
I-BES iyinhlanganisela ye-zwitterionic, okusho ukuthi inezindleko ezinhle nezimbi ngaphakathi kwesakhiwo sayo. Lesi sici siyivumela ukuthi igcine i-pH ezinzile ngempumelelo ezixazululweni.
I-BES inenani le-pKa elingaba ngu-7.4, okwenza ibe usizo kakhulu ekuqiniseni amazinga e-pH engokwenyama. Ivame ukusetshenziswa ekuhlolweni kwezinto eziphilayo kanye namakhemikhali, njengokuhlanzwa kwamaprotheni, ukusabela kwama-enzyme, kanye nokukhuliswa kwamaseli, lapho ukugcina i-pH ethile kubalulekile khona.
Ngaphezu kwalokho, i-BES isetshenziswa kabanzi kumasu e-electrophoresis, njengoba isiza ukugcina i-pH edingekayo ekuhlukaniseni nasekuhlaziyeni ama-biomolecule ashajiwe, njengeprotheni nama-nucleic acid.
