I-Phenylgalactoside CAS:2818-58-8

Umphumela emsebenzini we-enzyme: I-Phenylgalactoside ivame ukusetshenziswa ukukala umsebenzi we-enzyme i-β-galactosidase. Uma i-phenylgalactoside i-hydrolyzed yi-β-galactosidase, ikhipha i-p-nitrophenol. Ukuqongelela kwe-p-nitrophenol kungalinganiswa ngokwenani, kunikeze ukuqonda ngomsebenzi we-β-galactosidase. Lo mphumela usetshenziswa ezinhlotsheni ezifana nokuhlolwa kwe-enzyme kanye nezinhlelo zokuhlola.

Ukuhlaziywa kokubonakaliswa kwezakhi zofuzo: I-Phenylgalactoside ivame ukusetshenziswa njengesisekelo ekuhlolweni kwebhayoloji yama-molecule ukuze kufundwe ukubonakaliswa kwezakhi zofuzo. Izakhi zofuzo ze-lacZ, ezifaka ikhodi ye-β-galactosidase, zivame ukuhlanganiswa nokulandelana kokulawula kwezinye izakhi zofuzo ezithakaselwayo. Ukubonakaliswa kwezakhi zofuzo ze-lacZ kanye ne-hydrolysis ye-phenylgalactoside yi-β-galactosidase kungabonisa iphethini yokubonakaliswa kanye nezinga lezakhi zofuzo ezihlosiwe ezifundwayo.

Izinhlelo zokuhlola: I-Phenylgalactoside iyisici esibalulekile sezinhlelo zokuhlola ezisebenzisa umsebenzi we-β-galactosidase. Isibonelo esisodwa esaziwa kabanzi yindlela yokuhlola eluhlaza okwesibhakabhaka nomhlophe, esetshenziselwa ukuhlonza amaseli aphinde ahlanganiswe noma aguquliwe ekuhlolweni kwebhayoloji yama-molecular. Amakoloni aphumelele ekuthatheni i-DNA ephinde ihlanganiswe noma adlule ekuhlanganisweni kwezakhi zofuzo azoveza i-β-galactosidase, okuholela ekufakweni kwe-phenylgalactoside kanye nokwakheka kombala oluhlaza okwesibhakabhaka.

Ukuhlanzwa kwamaprotheni: Kwezinye izimo, i-phenylgalactoside ingasetshenziswa njenge-ligand ye-affinity chromatography ukuhlanza amaprotheni abopha ngokuqondile noma asebenza yi-β-galactosidase. Iphrotheni ethandwayo ingaba ne-affinity tag noma i-fusion tag equkethe isizinda esibopha i-β-galactosidase. Ngokudlulisa ingxube yamaprotheni kukholomu ene-phenylgalactoside engashukumi, iphrotheni efiselekayo ingagcinwa ngokukhetha futhi kamuva iphunyuzwe.