I-P-NITROPHENYL BETA-D-LACTOPYRANOSIDE CAS:4419-94-7

I-P-Nitrophenyl beta-D-lactopyranoside, eyaziwa nangokuthi i-PNPG, iyinhlanganisela evame ukusetshenziswa ekuhlolweni kwe-enzyme ukukala umsebenzi we-beta-galactosidase, i-enzyme ehilelekile ekusetshenzisweni kwe-carbohydrate. I-PNPG iyi-substrate yokwenziwa engahlukaniswa yi-beta-galactosidase, okuholela ekukhishweni komkhiqizo ophuzi. Ubukhulu be-substrate hydrolysis bungalinganiswa nge-spectrophotometric ngokulinganisa ukumuncwa komkhiqizo ku-wavelength ethile. Lokhu kuvumela abacwaningi ukuthi bahlole umsebenzi kanye ne-kinetics ye-beta-galactosidase ezimweni ezahlukahlukene, njengokufunda umsebenzi we-enzyme, ukuhlola ama-enzyme inhibitors noma ama-activator, noma ukuhlola imiphumela yokuguqulwa kwezakhi zofuzo emsebenzini we-enzyme.

Oda noma Cela Isilinganiso

Imininingwane Yomkhiqizo

Amathegi Omkhiqizo

Ukusetshenziswa kanye Nomphumela

Ukutholwa komsebenzi we-beta-galactosidase: I-PNPG ivame ukusetshenziswa ekuhlolweni ukuze kulinganiswe umsebenzi we-beta-galactosidase, i-enzyme evuselela i-hydrolysis ye-lactose ibe yi-glucose ne-galactose. I-hydrolysis ye-PNPG yi-beta-galactosidase ikhipha i-molecule ye-p-nitrophenol (pNP), engatholakala nge-spectrophotometric ngenxa yombala wayo ophuzi.

Ukuhlolwa kwama-enzyme inhibitors nama-activator: I-PNPG ingasetshenziswa ekuhlolweni okuphezulu ukuze kutholakale ama-compound alawula umsebenzi we-beta-galactosidase. Ngokulinganisa izinga le-PNPG hydrolysis lapho kukhona ama-compounds ahlukene okuhlola, abacwaningi bangathola ama-inhibitors anciphisa umsebenzi we-enzyme noma ama-activator athuthukisa umsebenzi we-enzyme.

Ucwaningo lwe-kinetics ye-enzyme: I-hydrolysis ye-PNPG yi-beta-galactosidase ilandela i-kinetics ye-Michaelis-Menten, okuvumela abacwaningi ukuthi bathole amapharamitha abalulekile e-enzyme njengejubane lokusabela eliphezulu (i-Vmax) kanye ne-Michaelis constant (Km). Lolu lwazi lusiza ekuqondeni ukufana kwe-substrate ye-enzyme kanye nokusebenza kahle kwe-catalytic.

Izicelo zebhayoloji yama-molecule: I-Beta-galactosidase, ehlukanisa i-PNPG, ivame ukusetshenziswa njenge-gene yentatheli ku-biology yama-molecule. I-substrate ye-PNPG ivame ukusetshenziselwa ukuthola nokubona ngeso lengqondo ukuvezwa kwe-gene yentatheli, okuhlinzeka ngendlela elula nebucayi yokuhlola ukuvezwa kwezakhi zofuzo ezinhlelweni ezahlukene zokuhlola.

Isampula Yomkhiqizo

Ukupakisha Umkhiqizo:

Ulwazi Olwengeziwe:

Ukwakheka	C18H25NO13
Ukuhlolwa	99%
Ukubukeka	Impuphu emhlophe
Inombolo ye-CAS	4419-94-7
Ukupakisha	Encane futhi enkulu
Impilo Yeshelufu	iminyaka engu-2
Isitoreji	Gcina endaweni epholile neyomile
Isitifiketi	I-ISO.