I-Tris maleate CAS:72200-76-1

Umthamo wokufaka i-buffer: I-Tris (i-maleate) iyi-buffer ye-pH ephumelelayo, okusho ukuthi ingamelana nezinguquko ku-pH ngokumunca noma ukukhulula ama-proton. Isetshenziswa kabanzi ukugcina uhla oluthile lwe-pH, ngokuvamile phakathi kwe-pH 6 no-8, ezinhlelweni ezahlukene zebhayoloji nezamakhemikhali.

Ucwaningo lwamaprotheni nama-enzyme: I-Tris (i-maleate) ivame ukusetshenziswa ezifundweni zamaprotheni nama-enzyme, lapho ukugcina i-pH ezinzile kubalulekile ekugcineni ukuzinza nokusebenza kwawo. Kungasiza ekulondolozeni ukwakheka kanye nokusebenza kwamaprotheni ngokuvimbela ukuguquka kwe-pH okubangelwa yi-pH.

Ukusetshenziswa kwe-molecular biology: I-Tris (i-maleate) isetshenziswa kabanzi nasemasu e-molecular biology njenge-DNA ne-RNA isolation, i-polymerase chain reaction (PCR), kanye ne-gel electrophoresis. Isiza ukugcina izimo ze-pH ezifanele ezidingekayo kulezi zinqubo futhi iqinisekisa ukunemba kwazo kanye nokuphindaphindeka kwazo.

Ukusetshenziswa kwezimboni: I-Tris (i-maleate) isetshenziswa ezinqubweni ezahlukene zezimboni njengokukhiqiza imithi, ukubilisa, kanye ne-biotechnology. Isetshenziselwa ukulawula i-pH ekukhiqizweni okukhulu, ukuqinisekisa izimo ezifanele zokukhula nomsebenzi wezinto ezincane noma ukuhlanganiswa kwemikhiqizo efiselekayo.

I-Analytical chemistry: I-Tris (i-maleate) isetshenziswa ku-analytical chemistry yokulinganisa kanye nokulinganisa amamitha e-pH, kanye nokulungiselela ama-calibration buffers okulinganisa i-pH. Ihlinzeka ngenani le-pH elaziwayo lokulinganisa okunembile nokuthembekile.