I-D-Luciferin CAS: 2591-17-5 Intengo Yomkhiqizi

I-D-Luciferin iyi-molecule encane ekhipha ukukhanya etholakala kakhulu ezintweni eziphilayo ezikhanyayo njenge-fireflies, amabhaktheriya, kanye nezinto eziphilayo zasolwandle. Iyingxenye ebalulekile yokusabela kwe-luciferase, inqubo yamakhemikhali ekhiqiza ukukhanya.

I-D-Luciferin ivame ukusetshenziswa njengesisekelo ekuhlolweni kwe-bioluminescence kanye namasu okuthwebula izithombe, lapho ukusabela kwayo ne-luciferase kukhiqiza ukukhanya okungatholakala futhi kulinganiswe. Lokhu kuyenza ithuluzi eliwusizo ezindaweni ezahlukene zocwaningo, okuhlanganisa i-molecular biology, ukutholakala kwezidakamizwa, kanye nokuthwebula izithombe kwezokwelapha.

Ngenxa yezakhiwo zayo ezihlukile zokubona, i-D-Luciferin isetshenziswe kabanzi ezifundweni ezihilela ukubonakaliswa kwezakhi zofuzo, ukusebenzisana kwamaprotheni namaphrotheni, kanye nokusabalalisa amaseli. Inikeza indlela engangenisi uhlaselo futhi ebucayi yokuphenya izinqubo zezinto eziphilayo ngesikhathi sangempela.

Ngaphezu kwalokho, kuye kwathuthukiswa ama-derivatives e-D-Luciferin nama-analog, okuvumela ukuguqulwa okuhlukahlukene kanye nezinhlelo zokusebenza. Lawa ma-derivatives aguquliwe avumela amaza ahlukahlukene okukhishwa kokukhanya, ukuqina okuthuthukisiwe, kanye nokungena kwezicubu okuthuthukisiwe.

Oda noma Cela Isilinganiso

Imininingwane Yomkhiqizo

Amathegi Omkhiqizo

Ukusetshenziswa kanye Nomphumela

Ukuthwebula izithombe ze-bioluminescence: I-D-Luciferin isetshenziswa kabanzi kumasu okuthwebula izithombe ze-bioluminescence, njengokuhlolwa kwentatheli ye-luciferase. Ngokungenisa izakhi zofuzo ze-luciferase emaseli noma ezintweni eziphilayo ezithakaselwayo, umsebenzi noma ukubonakaliswa kwezakhi zofuzo ezithile kungabonakala futhi kulinganiswe. Lobu buchwepheshe buvumela abacwaningi ukuthi balandelele futhi baqaphe izinqubo zamaseli ngesikhathi sangempela.

Ukutholakala kwemithi kanye nentuthuko: Ukuhlolwa kwe-bioluminescence okusekelwe ku-D-Luciferin kuyithuluzi elibalulekile ekutholakaleni kwemithi. Ngokufaka izakhi zofuzo ze-luciferase reporter kumaseli noma izinto eziphilayo eziqondiwe, abacwaningi bangalinganisa umphumela wemithi noma amakhemikhali ezinqubweni ezithile zebhayoloji. Lokhu kwenza kube nokwenzeka ukuhlonza abantu abangaba khona ngemithi kanye nokuhlolwa kokusebenza kwayo kahle.

Ucwaningo lwebhayoloji yama-molecule: I-D-Luciferin isetshenziswa kabanzi ezindleleni ezahlukene zebhayoloji yama-molecule, njengokufunda ukusebenzisana kwamaprotheni nama-protein, ukuqapha ukubonakaliswa kwezakhi zofuzo, kanye nokuhlola izindlela zokubonisana kwamaseli. Ikhono lokulinganisa ngobuningi ukukhishwa kokukhanya linikeza indlela engahlaseli futhi ebucayi yokuphenya lezi zinqubo zebhayoloji.

Ukuthwebula izithombe ze-in vivo: I-D-Luciferin ingasetshenziswa ekuthwebuleni izithombe ze-in vivo kumamodeli ezilwane. Ngokufaka i-D-Luciferin esilwaneni, amaseli noma izicubu eziveza i-luciferase zingabonakala futhi ziqashwe kusetshenziswa izinhlelo zokuthwebula izithombe ze-bioluminescence. Lokhu kuvumela abacwaningi ukuthi batadishe ukuqhubeka kwesifo, ukufuduka kwamaseli, kanye nezimpendulo zokwelapha ezilwaneni eziphilayo.

Ukuqapha imvelo: Izinhlelo ezisekelwe ku-D-Luciferin zisetshenziswe ekuqapheni imvelo, njengokuthola ukungcoliswa kwamagciwane ekudleni nasemanzini. Ukuvezwa kwezakhi zofuzo ze-Luciferase kumabhaktheriya kungasetshenziswa ukuhlola ukuphila kwawo kanye nomsebenzi wawo, okuhlinzeka ngendlela esheshayo nebucayi yokuqapha inani lamagciwane emvelo.

Ukupakisha Umkhiqizo:

Ulwazi Olwengeziwe:

Ukwakheka	C11H8N2O3S2
Ukuhlolwa	99%
Ukubukeka	Impuphu ephuzi ekhanyayo
Inombolo ye-CAS	2591-17-5
Ukupakisha	Encane futhi enkulu
Impilo Yeshelufu	iminyaka engu-2
Isitoreji	Gcina endaweni epholile neyomile
Isitifiketi	I-ISO.