I-N-Ethylmaleimide CAS:128-53-0 Intengo Yomkhiqizi

Ukuguqulwa Kwamaprotheni: I-NEM iguqula ngokungenakuguqulwa izinsalela ze-cysteine ​​​​kumaprotheni ngokwenza i-covalent adduct. Lokhu kuguqulwa kungathinta umsebenzi weprotheni, isakhiwo, kanye nokusebenzisana. Ukusabela kwe-NEM namaqembu e-sulfhydryl kuyenza ithuluzi eliwusizo lokufunda umsebenzi weprotheni kanye nezinqubo ezincike ku-thiol.

Ukuvinjelwa kwama-Enzyme: Ama-enzyme amaningi athembele kumaqembu e-thiol ukuze enze umsebenzi wawo wokuvuselela. Ngokusabela kumaqembu e-thiol, i-NEM ingavimbela ama-enzyme athile ngokukhetha. Lokhu kuye kwasetshenziswa kabanzi ukutadisha i-kinetics ye-enzyme, ukuqonda izindlela ze-enzymatic, kanye nokuhlola izithibi ze-enzyme ezingaba khona.

Izifundo Zokusebenzisana Kwamaprotheni: I-NEM ivame ukusetshenziselwa ukuphenya ukusebenzisana kwamaprotheni. Ngokuvimba ngokukhetha izinsalela ze-cysteine ​​ezisebenzayo, i-NEM ivimbela ukwakheka kwezibopho ze-disulfide phakathi kwamaprotheni asebenzisanayo, ngaleyo ndlela kuphazamise ukusebenzisana kwawo. Lokhu kuvumela abacwaningi ukuthi batadishe indima yama-cysteine ​​athile ekusebenzisaneni kwamaprotheni namaprotheni.

Izifundo Zokulawulwa Kwe-Redox: I-NEM isetshenziswa ekutadisheni izindlela zokulawulwa kwe-redox kumaphrotheni. Ngokushintsha ama-cysteine ​​asebenzayo, i-NEM ingazinzisa ukwakheka kwamaprotheni athile futhi ivimbele ukungcoliswa okungafuneki noma ukuncipha kwezinsalela ze-cysteine. Lokhu kusiza ekuqondeni indima yezindlela zokulawula ze-redox kanye nezindlela zomthetho ezizwela i-redox.

Izifundo Zokuxhumanisa: I-NEM ingasetshenziswa njenge-ejenti yokuxhumanisa ukuze kuzinziswe ama-protein complexes noma ukusebenzisana kwama-protein nama-protein. Ngokushintsha ama-cysteine ​​residues, i-NEM ingadala izibopho ezihambisanayo phakathi kwama-protein asebenzisanayo, okuvumela abacwaningi ukuthi baphenye ama-protein complexes kanye nezakhiwo zawo. Le ndlela iwusizo kakhulu ezifundweni zebhayoloji yesakhiwo kanye ne-proteomics.

Ukutholakala Kwemithi: Ngenxa yekhono layo lokuxhumana ngokukhetha nezinsalela ze-cysteine, i-NEM isetshenziswe ocwaningweni lokutholakala kwemithi. Ingasetshenziswa ukuhlonza nokuchaza izinhloso zemithi ezingaba khona noma ukutadisha ukucaciswa kwemithi engaba khona kumaprotheni acebile nge-cysteine.