I-4-Nitrophenyl Beta-D-glucuronide CAS:10344-94-2

Ukutholwa komsebenzi we-β-glucuronidase: I-4-NPBG ivame ukusetshenziswa njenge-substrate ye-chromogenic ukuhlola ubukhona nomsebenzi we-β-glucuronidase kumasampuli ahlukahlukene ezinto eziphilayo.I-enzyme ihlukanisa isibopho se-glycosidic se-4-NPBG, ikhiqiza i-4-nitrophenol, engalinganiswa kusetshenziswa i-spectrophotometry.

Ucwaningo lwe-metabolism yezidakamizwa: Njengoba i-β-glucuronidase idlala indima ebalulekile ekusetshenzisweni kwezidakamizwa kanye ne-xenobiotics, i-4-NPBG ingasetshenziswa ukuhlola umsebenzi wale enzyme ezifundweni ze-metabolism yezidakamizwa.Kuyasiza ekuqondeni izinga kanye ne-kinetics yokusabela kwe-glucuronidation, okubalulekile ekukhishweni kwezidakamizwa kanye ne-bioavailability.

Izifundo ze-Toxicology: Ezinye izinhlanganisela ezinobuthi zingagaywa futhi zikhishwe ngendlela ye-glucuronide conjugates.Ngokusebenzisa i-4-NPBG njenge-substrate, abacwaningi bangahlola umsebenzi we-β-glucuronidase kumathishu ahlukene noma imigqa yamaseli ukuze bahlole ubuthi obungaba khona noma imiphumela emibi yezinhlanganisela.

Ukuxilongwa komtholampilo: Ukulinganisa komsebenzi we-β-glucuronidase usebenzisa i-4-NPBG kungasetshenziswa kuzilungiselelo zomtholampilo ukuze kuhlolwe izifo noma izimo ezithile.Amazinga angavamile noma umsebenzi we-β-glucuronidase angase abonise ukuphazamiseka okuthile kofuzo, ukungasebenzi kahle kwesibindi, noma umdlavuza.