I-4-Methylumbelliferyl-beta-D-glucopyranoside CAS:18997-57-4

Umphumela we-4-Methylumbelliferyl-beta-D-glucopyranoside (MUG) ukusebenza njengesisekelo se-enzyme beta-glucosidase. Le enzyme ihlukanisa isibopho se-glucosidic se-MUG, okuholela ekukhishweni kwe-4-Methylumbelliferone (4-MU). Ukusetshenziswa kwe-MUG kugxile kakhulu emkhakheni we-microbiology, ikakhulukazi ekutholeni nasekuhlonzeni amabhaktheriya akhiqiza i-beta-glucosidase. I-MUG ivame ukusetshenziswa ekutholeni i-Escherichia coli (E. coli) emanzini nasezilwaneni. I-E. coli ine-enzyme beta-glucosidase, engakwazi ukuhlanza i-MUG futhi ikhiqize isignali ye-fluorescent lapho kukhona ukukhanya kwe-ultraviolet (UV). Impahla ye-fluorescent ye-4-MU ivumela ukutholakala nokulinganiswa okulula. Lapho i-substrate ye-MUG ihlanzwa, i-4-MU ekhiqizwayo ikhipha ukukhanya okuluhlaza okwesibhakabhaka, okwenza kube lula ukuhlonza amabhaktheriya anomsebenzi we-beta-glucosidase. Le ndlela ivame ukusetshenziswa ekuqapheni imvelo kanye nokuhlolwa kokuphepha kokudla, njengoba inikeza indlela esheshayo nebucayi yokuthola ukungcola kwamagciwane. Ngaphezu kokusetshenziswa kwayo ku-microbiology, i-MUG ingasetshenziswa futhi ukutadisha umsebenzi kanye nokuvinjelwa kwe-beta-glucosidase ocwaningweni lwe-biochemistry kanye ne-molecular biology. I-fluorescence yayo ivumela ukulinganiswa kwe-enzyme kinetics futhi ingasetshenziswa ukuhlola izithiyo ezingaba khona noma iziqalisi zomsebenzi we-beta-glucosidase. Sekukonke, i-MUG iyinhlanganisela eguquguqukayo ethola ukusetshenziswa kabanzi emikhakheni ye-microbiology, i-biochemistry, kanye ne-molecular biology ukuthola umsebenzi we-beta-glucosidase kanye nokuhlonza amagciwane akhiqiza le enzyme.