I-4-Nitrophenyl Beta-D-glucuronide CAS:10344-94-2

I-4-Nitrophenyl Beta-D-glucuronide iyinhlanganisela yamakhemikhali eyakhiwe ngokunamathisela i-molecule ye-glucose eqenjini le-4-nitrophenyl ngokusebenzisa ukuxhumana kwe-glycosidic. Ivame ukusetshenziswa njenge-substrate ekuhlolweni kwe-enzymatic ukuthola ubukhona kanye nomsebenzi we-β-glucuronidase, i-enzyme ehilelekile ekusetshenzisweni kwemithi ehlukahlukene kanye nama-xenobiotics ezincelisayo. Uma i-β-glucuronidase ikhona, ihlukanisa isibopho se-glycosidic phakathi kweqembu le-glucose ne-4-nitrophenyl, okuholela ekukhishweni kwe-4-nitrophenol, engatholakala nge-spectrophotometric ku-400-420 nm. Lokhu kusabela kwe-enzymatic kunikeza isilinganiso sobuningi somsebenzi we-β-glucuronidase futhi kuvame ukusetshenziswa njengethuluzi ekutholakaleni kwemithi, izifundo zobuthi, kanye nokuxilongwa kwemitholampilo.

Oda noma Cela Isilinganiso

Imininingwane Yomkhiqizo

Amathegi Omkhiqizo

Ukusetshenziswa kanye Nomphumela

Ukutholwa komsebenzi we-β-glucuronidase: I-4-NPBG ivame ukusetshenziswa njenge-substrate ye-chromogenic ukuhlola ubukhona kanye nomsebenzi we-β-glucuronidase kumasampula ahlukahlukene ebhayoloji. I-enzyme ihlukanisa isibopho se-glycosidic se-4-NPBG, ikhiqize i-4-nitrophenol, engalinganiswa kusetshenziswa i-spectrophotometry.

Izifundo ze-metabolism yezidakamizwa: Njengoba i-β-glucuronidase idlala indima ebalulekile ekusetshenzisweni kwezidakamizwa kanye nama-xenobiotic, i-4-NPBG ingasetshenziswa ukuhlola umsebenzi wale enzyme ezifundweni ze-metabolism yezidakamizwa. Kuyasiza ekuqondeni ubukhulu kanye ne-kinetics ye-glucuronidation reactions, okubalulekile ekususweni kwemithi kanye nokutholakala kwayo emzimbeni.

Izifundo zobuthi: Amanye amakhemikhali anobuthi angaguqulwa abe yi-metabolite futhi akhishwe ngesimo se-glucuronide conjugates. Ngokusebenzisa i-4-NPBG njenge-substrate, abacwaningi bangahlola umsebenzi we-β-glucuronidase ezicutshini ezahlukene noma emigqeni yamaseli ukuze bahlole ubuthi obungaba khona noma imiphumela emibi yamakhemikhali.

Ukuxilongwa kwezokwelapha: Ukulinganiswa komsebenzi we-β-glucuronidase kusetshenziswa i-4-NPBG kungasetshenziswa ezindaweni zezokwelapha ukuxilonga izifo noma izimo ezithile. Amazinga noma umsebenzi ongavamile we-β-glucuronidase kungabonisa izifo ezithile zofuzo, ukungasebenzi kahle kwesibindi, noma umdlavuza.

Isampula Yomkhiqizo

Ukupakisha Umkhiqizo:

Ulwazi Olwengeziwe:

Ukwakheka	C12H13NO9
Ukuhlolwa	99%
Ukubukeka	Impuphu emhlophe
Inombolo ye-CAS	10344-94-2
Ukupakisha	Encane futhi enkulu
Impilo Yeshelufu	iminyaka engu-2
Isitoreji	Gcina endaweni epholile neyomile
Isitifiketi	I-ISO.