4-NITROPHENYL-ALPHA-D-MANNOPYRANOSIDE CAS:10357-27-4

Ama-substrate e-enzyme: I-4NPM ingasetshenziswa njenge-substrate yama-enzyme ahlukahlukene, kufaka phakathi ama-glycosidases nama-enzyme ahlobene nawo. Lawa ma-enzyme ahlukanisa isibopho se-glycosidic phakathi kwe-mannose ne-4NPM, okuholela ekukhululweni kwe-nitrophenyl moiety. Ububanzi be-substrate hydrolysis bungalinganiswa nge-spectrophotometric ngokuqapha ukumuncwa kweqembu le-nitrophenyl elikhishwe ku-wavelength ethile. Lokhu kuvumela abacwaningi ukuthi bahlole umsebenzi we-enzyme kanye ne-kinetics.

Ukuhlolwa kwe-metabolism yama-carbohydrate: Ngokusebenzisa i-4NPM njenge-substrate, abacwaningi bangacwaninga umsebenzi wama-enzyme ahilelekile ku-metabolism yama-carbohydrate, njenge-alpha-mannosidases. Lawa ma-enzyme afaka i-hydrolyze izibopho ze-glycosidic kuma-compounds aqukethe i-mannose, futhi umsebenzi wawo ungalinganiswa ngokuqapha ukukhishwa kwe-nitrophenyl moiety kusuka ku-4NPM.

Izifundo ze-Glycosylation: I-4NPM ingasetshenziswa futhi ekuhlolweni ukuze kuhlolwe ama-enzyme ahilelekile ezinqubweni ze-glycosylation. I-Glycosylation inqubo yokunamathisela ama-molecule kashukela kumaprotheni noma kwamanye ama-molecule, futhi ama-enzyme amaningi ahilelekile kule nqubo. Ngokusebenzisa i-4NPM njenge-substrate yokwamukela, abacwaningi bangalinganisa ukudluliselwa kwengxenye kashukela ku-4NPM ngama-enzyme athile ahilelekile ekuphenduleni kwe-glycosylation.

Ukuhlolwa kwama-enzyme inhibitors noma ama-activator: I-4NPM ingasetshenziswa ekuhlolweni kokuhlolwa okuphezulu ukuze kutholakale ama-compounds avimbela noma asebenze ama-enzyme athile. Ngokuhlola umphumela wama-test compounds ekufakweni kwe-hydrolysis noma ekuguqulweni kwe-4NPM ngama-enzyme aqondiwe, abacwaningi bangabona ama-ejenti okwelapha angaba khona noma ama-probe amakhemikhali awusizo okutadisha umsebenzi wama-enzyme.