3-HYDROXY-4-(5-NITROPYRIDYLAZO)PROPYLANI CAS:143205-66-7

Kuyi-fluorescent probe equkethe iqembu le-nitrobenzoxadiazole (NBD), elibonisa izakhiwo eziqinile ze-fluorescence.Iqembu le-aldehyde livumela ukulebula okuqondile nokutholwa kwama-biomolecules afana namaprotheni, ama-nucleic acid, nama-lipids.

I-NBD-aldehyde ivame ukusetshenziswa njengodayi osebenzayo ekuhlolweni kokuthola kanye nokulinganisa ukusabela kwe-protein noma i-peptide conjugation, ukuguqulwa kwamaprotheni, kanye nokusebenzisana kwamaprotheni-amaprotheni.Ingalebula amaqembu amahhala e-amino kumaprotheni noma ama-peptide, yakhe izibopho eziqinile eziqinile.Lokhu kwenza kube usizo ezinhlelweni ezifana ne-immunocytochemistry, immunohistochemistry, kanye ne-western blotting.

I-fluorescence ye-NBD-aldehyde ingajatshuliswa nge-ultraviolet noma ukukhanya okuluhlaza okwesibhakabhaka, futhi ikhipha i-fluorescence eluhlaza.Lesi sakhiwo sinika amandla ukubonwa kanye nokulinganisa kwama-biomolecules anelebuli kusetshenziswa i-fluorescence microscopy noma amasu e-spectroscopy.