3-HYDROXY-4-(5-NITROPYRIDYLAZO)PROPYLANI CAS:143205-66-7

Kuyi-probe ye-fluorescent equkethe iqembu le-nitrobenzoxadiazole (NBD), elibonisa izakhiwo eziqinile ze-fluorescence. Iqembu le-aldehyde livumela ukulebula okukhethekile kanye nokutholakala kwama-biomolecule afana namaprotheni, ama-nucleic acid, kanye nama-lipid.

I-NBD-aldehyde ivame ukusetshenziswa njengodayi ophendulayo ekuhlolweni kokuthola nokulinganisa ukusabela kokuhlanganiswa kwamaprotheni noma ama-peptide, ukuguqulwa kwamaprotheni, kanye nokusebenzisana kwamaprotheni nama-protein. Ingabhala amaqembu ama-amino amahhala kumaphrotheni noma ama-peptide, yakha izibopho eziqinile ze-covalent. Lokhu kuyenza ibe usizo ezinhlelweni ezifana ne-immunocytochemistry, i-immunohistochemistry, kanye ne-western blotting.

Ukugqama kwe-NBD-aldehyde kungavuselelwa ngokukhanya kwe-ultraviolet noma okuluhlaza okwesibhakabhaka, futhi kukhipha ukukhanya okuluhlaza. Lesi sici senza kube lula ukubona nokulinganisa ama-biomolecule anelebula kusetshenziswa ubuchwepheshe be-fluorescence microscopy noma i-spectroscopy.