I-X-GAL CAS:7240-90-6 Intengo Yomkhiqizi

Ukushintsha Umbala: I-X-Gal ngokuvamile ayinambala kodwa, lapho i-hydrolysis nge-β-galactosidase, iphenduka ibe luhlaza okwesibhakabhaka.Lokhu kushintsha kombala kuvumela ukutholwa okubonakalayo kanye nokulinganisa komsebenzi we-β-galactosidase.

Ukutholwa kofuzo lwe-LacZ: I-X-Gal isetshenziselwa ukukhomba amaseli noma izakhi zofuzo eziveza isakhi sofuzo se-lacZ.I-LacZ ivame ukusetshenziswa njengofuzo lwentatheli kubhayoloji yamangqamuzana ukuhlola ukusho kofuzo noma umsebenzi womgqugquzeli wocwaningo.

Ukuhlolwa Kwekholoni: I-X-Gal ivame ukusetshenziswa ekuhlolweni kokuhlolwa kwekholoni yebhaktheriya.Amakholomu ebhaktheriya aveza i-LacZ abonakala eluhlaza okwesibhakabhaka uma ekhuliswe ku-agar equkethe i-X-Gal, okwenza kube lula ukuhlonza nokukhethwa kwamakoloni ane-lacZ-positive.

Ukuhlaziywa kwe-Gene Fusion: I-X-Gal nayo iyasetshenziswa ekuhloleni ukuhlanganisa izakhi zofuzo.Uma isakhi sofuzo esiqondiwe sixhunywe kufuzo lwe-lacZ, ukungcoliswa kwe-X-Gal kungaveza iphethini yenkulumo yephrotheni ehlanganisiwe ngaphakathi kweseli noma izicubu.

I-Protein Localization: I-X-Gal staining ingasetshenziselwa ukuphenya ukwenziwa kwasendaweni kwamaprotheni angaphansi kwamaselula.Ngokuhlanganisa iphrotheni enentshisekelo kuzakhi zofuzo ze-lacZ, umsebenzi we-β-galactosidase ungabonisa lapho iphrotheni itholakala khona ngaphakathi kweseli.

Ama-X-Gal Analogue: Amafomu alungisiwe e-X-Gal, afana ne-Bluo-Gal noma i-Red-Gal, athuthukisiwe ukuze avumele ezinye izikimu zokuthuthukisa imibala.Lawa ma-analogue anika amandla umehluko phakathi kwamaseli noma izicubu ezine-lacZ-positive kanye ne-lacZ-negative esebenzisa imibala ehlukene.