I-4-Nitrophenyl-alpha-D-galactopyranoside CAS:7493-95-0

I-substrate yokuhlola i-enzyme: I-4-Nitrophenyl-alpha-D-glucopyranoside ivame ukusetshenziswa njenge-substrate ekuhlolweni kwe-enzyme ukukala umsebenzi wama-glycosidases namanye ama-enzyme ahilelekile ekugayweni kwe-carbohydrate. Lawa ma-enzyme angahlukanisa isibopho phakathi kwe-glucose neqembu le-4-nitrophenyl, akhiphe umkhiqizo ophuzi obizwa ngokuthi i-4-nitrophenol. Inani le-4-nitrophenol elikhiqizwayo lilingana ngqo nomsebenzi we-enzyme, okuvumela ukulinganiswa kobuningi bomsebenzi we-enzyme.

Ukutholwa komsebenzi we-enzyme: Ngokusebenzisa i-4-Nitrophenyl-alpha-D-glucopyranoside njenge-substrate, umsebenzi wama-enzyme athile ungalinganiswa. Isibonelo, ama-enzyme e-glycosidase afana ne-beta-glucosidase, i-alpha-glucosidase, noma i-beta-galactosidase angahlukanisa i-compound, akhiphe i-4-nitrophenol, engatholakala nge-photometric. Le ndlela isetshenziswa kabanzi ezinhlotsheni ezahlukene zezokwelapha, zemithi, kanye ne-biotechnology.

Ukuhlolwa komthamo ophezulu: Ngenxa yokuhambisana kwayo nezinhlelo zokuthola ezenzakalelayo, i-4-Nitrophenyl-alpha-D-glucopyranoside ivame ukusetshenziswa ekuhlolweni komthamo ophezulu. Le khompawundi ivumela ukuhlukaniswa okusheshayo nokusebenza kahle komsebenzi we-enzyme, okwenza kube usizo ekutholakaleni kwemithi, ubunjiniyela be-enzyme, kanye nezifundo zokuvimbela ama-enzyme.

Izicelo zokuxilonga: I-4-Nitrophenyl-alpha-D-glucopyranoside ingasetshenziswa ezinhlelweni zokuxilonga ukuthola ukuba khona noma umsebenzi wama-enzyme athile ahlobene nezifo ezahlukahlukene. Isibonelo, umsebenzi wama-enzyme athile ungasetshenziswa njengophawu lokuxilonga lwezifo ezithile, kanti i-4-Nitrophenyl-alpha-D-glucopyranoside ingasetshenziswa njengesisekelo sokulinganisa umsebenzi wala ma-enzyme kumasampula omtholampilo.