I-X-GAL CAS: 7240-90-6 Intengo Yomkhiqizi

Ukushintsha Kombala: I-X-Gal ngokuvamile ayinambala kodwa, lapho i-hydrolysis ikhishwa yi-β-galactosidase, iphenduka ibe luhlaza okwesibhakabhaka. Lokhu kushintsha kombala kuvumela ukubona okubonakalayo kanye nokulinganisa umsebenzi we-β-galactosidase.

Ukutholwa Kwezakhi Zofuzo ze-LacZ: I-X-Gal isetshenziselwa ukuhlonza amaseli noma izakhi zofuzo eziveza izakhi zofuzo ze-lacZ. I-LacZ ivame ukusetshenziswa njengezakhi zofuzo ezibikayo ku-molecular biology ukuhlola ukubonakaliswa kwezakhi zofuzo noma umsebenzi wokutadisha wokukhuthaza.

Ukuhlolwa Kwekoloni: I-X-Gal ivame ukusetshenziswa ekuhlolweni kwekoloni lamagciwane. Amakoloni amagciwane aveza i-LacZ abonakala eluhlaza okwesibhakabhaka uma ekhuliswe ku-agar equkethe i-X-Gal, okwenza kube lula ukuhlonza nokukhetha amakoloni ane-lacZ.

Ukuhlaziywa Kokuhlanganiswa Kwezakhi Zofuzo: I-X-Gal isetshenziswa futhi ekuhlolweni kokuhlanganiswa kwezakhi zofuzo. Uma i-gene eqondiwe ixhunywe ku-gene ye-lacZ, ukudaya kwe-X-Gal kungaveza iphethini yokubonakaliswa kweprotheni yokuhlanganiswa ngaphakathi kweseli noma izicubu.

Ukutholakala Kwamaprotheni: Ukudaya kwe-X-Gal kungasetshenziswa ukuhlola ukutholakala kwamaprotheni angaphansi kweseli. Ngokuhlanganisa iphrotheni ethakazelisayo ne-lacZ gene, umsebenzi we-β-galactosidase ungabonisa ukuthi iphrotheni itholakala kuphi ngaphakathi kweseli.

Ama-analogue e-X-Gal: Izinhlobo eziguquliwe ze-X-Gal, njenge-Bluo-Gal noma i-Red-Gal, zakhiwe ukuze kuvunyelwe izinhlelo zokuthuthukisa imibala ehlukile. Lezi zinhlobo ezifanayo zivumela ukuhlukanisa phakathi kwamaseli noma izicubu ezine-lacZ-positive kanye ne-lacZ-negative kusetshenziswa imibala ehlukene.