I-HEPES CAS: 7365-45-9 Intengo Yomkhiqizi

I-HEPES (4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid) iyi-buffer yezinto eziphilayo evame ukusetshenziswa ocwaningweni lwezinto eziphilayo nezamakhemikhali. Yaziwa ngekhono layo lokugcina i-pH ezinzile ezixazululweni zamanzi, okwenza ifaneleke ezinhlelweni ezahlukahlukene ezibandakanya izinqubo zamaseli kanye ne-enzymatic. I-HEPES iyinhlanganisela ye-zwitterionic, okusho ukuthi inezindleko ezinhle nezimbi emaqenjini ahlukene asebenzayo, okuyivumela ukuthi imelane nezinguquko ku-pH ezibangelwa ukwengezwa kwama-acid noma izisekelo. Ivame ukusetshenziswa ekukhuleni kwamaseli, ekuhlolweni kwama-enzyme, ezifundweni zamaphrotheni, ekuhlolweni kwe-electrophoresis, kanye nasekusetshenzisweni kwemithi.

Oda noma Cela Isilinganiso

Imininingwane Yomkhiqizo

Amathegi Omkhiqizo

Ukusetshenziswa kanye Nomphumela

Ukuvimba i-pH: I-HEPES isetshenziswa kabanzi njenge-ejenti yokuvimba ukugcina i-pH ezinzile kumidiya yokukhulisa amaseli kanye nokuhlolwa kwezinto eziphilayo. Isebenza kahle ebangeni le-pH yezinto eziphilayo eliphakathi kuka-6.8 no-8.2, okwenza ifanelekele ukugcina izimo ezifanele zezinqubo zezinto eziphilayo.

Ukukhuliswa kwamaseli: I-HEPES ivame ukungezwa kwimidiya yokukhuliswa kwamaseli ukuze kuzinziswe izinga le-pH, inikeze indawo efanelekile yokukhula kwamaseli nokugcina ukusebenza kwamaseli. Kuyasiza ekuvimbeleni ukuguquguquka kwe-pH okungathinta kabi ukuziphatha kwamaseli kanye nemiphumela yokuhlola.

Ukuhlolwa kwe-enzyme: I-HEPES ivame ukusetshenziswa njenge-buffer ekuphenduleni kwe-enzyme ngenxa yekhono layo lokugcina i-pH ethile. Iyasiza ekugcineni umsebenzi we-enzyme ofanele kanye nokuzinza, ngaleyo ndlela ithuthukise ukunemba nokuthembeka kokuhlolwa kwe-enzyme.

Izifundo zamaphrotheni: I-HEPES isetshenziswa ezivivinyweni ezahlukahlukene ezihlobene namaphrotheni, okuhlanganisa ukuhlanzwa kwamaphrotheni, ukwakheka kwamaphrotheni, kanye nokuhlaziywa kwesakhiwo samaphrotheni. Kuyasiza ekuqiniseni i-pH nokugcina ukugoqeka kwamaphrotheni okulungile kanye nokuqina phakathi nalezi zivivinyo.

I-Electrophoresis: I-HEPES ithola ukusetshenziswa kumasu e-gel electrophoresis, njenge-SDS-PAGE kanye ne-agarose gel electrophoresis. Isetshenziswa njenge-ejenti yokubopha ku-buffer egijimayo, ihlinzeka ngendawo ye-pH ezinzile yokuhlukaniswa nokuhlaziywa kwama-biomolecule.

Izakhi Zemithi: I-HEPES isetshenziswa ekwakhiweni kwemikhiqizo yemithi neye-biotechnology. Isiza ukugcina ukuzinza nobuqotho bezithako ezisebenzayo, iqinisekisa ukusebenza kwazo kanye nokuphila kwazo eshalofini.

Ukupakisha Umkhiqizo:

Ulwazi Olwengeziwe:

Ukwakheka	C8H18N2O4S
Ukuhlolwa	99%
Ukubukeka	Impuphu emhlophe
Inombolo ye-CAS	7365-45-9
Ukupakisha	Encane futhi enkulu
Impilo Yeshelufu	iminyaka engu-2
Isitoreji	Gcina endaweni epholile neyomile
Isitifiketi	I-ISO.