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I-N,N-Bis(2-hydroxyethyl) -2-aminoethanesulfonic acid sodium usawoti CAS:66992-27-6
I-N,N-Bis(2-hydroxyethyl) -2-aminoethanesulfonic acid usawoti we-sodium, owaziwa nangokuthi usawoti we-sodium we-HEPES, uyinhlanganisela yamakhemikhali evamise ukusetshenziswa njenge-ejenti egcina i-pH kumalabhorethri ebhayoloji namakhemikhali.Isiza ukugcina ububanzi be-pH obuzinzile, buyenze ilungele izinhlelo zokusebenza ezifana nesiko lamaseli, ukuhlolwa kwe-enzyme, izifundo zamaprotheni, i-electrophoresis, nokwakheka kwemithi.Usawoti we-sodium we-HEPES uqinisekisa izimo ezifanele zezinqubo zebhayoloji futhi uthuthukisa ukunemba nokuthembeka kwemiphumela yokuhlola.
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I-S-Butyrylthiocholine iodide CAS:1866-16-6
I-S-Butyrylthiocholine iodide iyinhlanganisela yamakhemikhali evame ukusetshenziswa ekuhlolweni kwamakhemikhali ezinto eziphilayo kanye ne-enzymatic.Kuyi-substrate ye-enzyme i-butyrylcholinesterase (BChE) futhi isetshenziselwa ukukala umsebenzi wayo.
Uma i-iodide ye-S-Butyrylthiocholine i-hydrolyzed yi-BChE, ikhiqiza i-thiocholine ne-butyric acid njengemikhiqizo.Ukukhululwa kwe-thiocholine kungalinganiswa kusetshenziswa i-spectrophotometric noma i-fluorometric assay, okuvumela ukulinganisa komsebenzi we-BChE.
I-iodide ye-S-Butyrylthiocholine ivamise ukusetshenziswa kuzilungiselelo zomtholampilo nezocwaningo ukuze kuhlolwe umsebenzi we-BChE kumasampuli afana ne-plasma yegazi noma izicubu.Ingasetshenziselwa ukuhlola umsebenzi we-enzymatic we-BChE kanye nendima yayo engaba khona ezinqubweni ezihlukahlukene zebhayoloji, kanye nasekuxilongweni nasekuqaphelweni kwezimo ezithile zezokwelapha.
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I-ABTS (2,2′-Azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) usawoti we-diammonium) CAS:30931-67-0
I-Diammonium 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonate), evame ukubizwa ngokuthi i-ABTS, i-chromogenic substrate evame ukusetshenziswa ekuhlolweni kwamakhemikhali ezinto eziphilayo, ikakhulukazi emkhakheni we-enzymology.Kuyinhlanganisela yokwenziwa esetshenziselwa ukukala umsebenzi wama-enzyme ahlukahlukene, kufaka phakathi ama-peroxidase nama-oxidase.
I-ABTS ayinambala ngesimo sayo se-oxidized kodwa iphenduka ibe luhlaza okwesibhakabhaka-luhlaza lapho ifakwa i-enzyme lapho kukhona i-hydrogen peroxide noma umoya-mpilo wamangqamuzana.Lokhu kushintsha kombala kungenxa yokwakheka kwe-cation radical, edonsa ukukhanya ku-spectrum ebonakalayo.
Ukusabela phakathi kwe-ABTS kanye ne-enzyme kukhiqiza umkhiqizo onemibala ongakalwa nge-spectrophotometrically.Ukuqina kombala kuhambisana ngokuqondile nomsebenzi we-enzymatic, okuvumela abacwaningi ukuthi bahlole ngobuningi i-enzyme kinetics, inhibition ye-enzyme, noma ukusebenzisana kwe-enzyme-substrate.
I-ABTS inohlu olubanzi lwezinhlelo zokusebenza emikhakheni eyahlukene, okuhlanganisa ukuxilonga emitholampilo, ucwaningo lwezemithi, nesayensi yokudla.Izwela kakhulu futhi inikeza ububanzi obubanzi obuguquguqukayo, okuyenza ibe ukukhetha okudumile ezivivinyweni eziningi ze-biochemical.
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4-NITROPHENYLΑ-D-MALTOHEXAOSIDE CAS:74173-30-1
I-4-Nitrophenyl α-D-maltohexaoside iyinhlanganisela eyingxenye yesigaba sokuxhumanisa kwe-α-glycosidic.Iphuma ku-maltose, okuyi-disaccharide eyakhiwe amayunithi amabili eglucose.Kule nhlanganisela, iqembu le-hydroxyl leyunithi yokuqala ye-glucose lithathelwa indawo yi-nitrophenyl moiety.
Le nhlanganisela ivame ukusetshenziswa njenge-substrate ekuhlolweni kwe-enzymatic ukutadisha umsebenzi wama-enzyme ahlukahlukene, ikakhulukazi lawo abandakanyeka ku-carbohydrate metabolism.Iqembu le-nitrophenyl livumela ukutholwa kalula kanye nokulinganisa ukusabela kwe-enzymatic ngokukala ukumunca noma i-fluorescence yomkhiqizo oqhekekile.
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PIPES CAS:5625-37-6 Intengo Yomkhiqizi
PIPES (piperazine-1,4-bisethanesulfonic acid) iyinhlanganisela ye-zwitterionic buffering evame ukusetshenziswa ocwaningweni lwebhayoloji nolwe-biochemical.Iyisivikelo se-pH esisebenzayo esinamandla aphezulu wokugcina izimo ze-pH ezinzile ebangeni le-pH lika-6.1 kuya ku-7.5.I-PIPES inokuphazanyiswa okuncane kwama-biomolecules futhi ifanele ukuhlolwa okuncike kukushisa.Ivame ukusetshenziswa kumasu we-gel electrophoresis kanye nokwakhiwa kwemithi njenge-ejenti yokuzinzisa.Sekukonke, PIPES iyinhlanganisela eguquguqukayo futhi esetshenziswa kabanzi kuzilungiselelo ezahlukahlukene zokuhlola.
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3,3′,5,5′-Tetramethylbenzidine CAS:207738-08-7
I-3,3′,5,5′-Tetramethylbenzidine, eyaziwa nangokuthi i-TMB, iyinhlanganisela yamakhemikhali evame ukusetshenziswa njenge-chromogenic substrate ekuhlolweni kwe-enzyme-linked immunosorbent (ELISA) nokunye ukuhlola kwamakhemikhali ezinto eziphilayo.Ivamise ukusetshenziselwa ukuthola kanye nokulinganisa ubukhona bama-enzyme afana ne-horseradish peroxidase (HRP) kumasampuli ahlukahlukene ebhayoloji.I-TMB ishintsha umbala usuka ongenambala ube luhlaza okwesibhakabhaka uma kukhona la ma-enzyme.Ngokulandelayo, ukusabela kungamiswa ngokungeza i-asidi eguqula umbala oluhlaza okwesibhakabhaka ube umbala ophuzi wokugcina.Amandla ombala ophuzi alingana nenani le-enzyme ekhona, okuvumela ukulinganisa.
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I-APS-5 CAS:193884-53-6 Intengo Yomkhiqizi
(4-Chlorophenyl) i-thio-methanol 1-(i-dihydrogen phosphate) usawoti we-disodium (1:2) iyinhlanganisela yamakhemikhali eyingxenye ye-acridine derivatives.Iqukethe uhlelo lwendandatho ye-10-methylacridine neqembu le-thioether elixhunywe endaweni ye-4-chlorophenyl.I-compound ibuye ibe neqembu le-methanol namaqembu amabili e-phosphate ahlukaniswe kancane ngama-ion sodium.
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5-Bromo-4-chloro-3-indolyl phosphate disodium usawoti CAS:102185-33-1
I-5-Bromo-4-chloro-3-indolyl phosphate disodium salt (BCIP) iyinhlanganisela yamakhemikhali evamise ukusetshenziswa ku-molecular biology kanye ne-biochemistry application.Kuyi-substrate ye-chromogenic yama-enzyme e-alkaline phosphatase.
I-BCIP ivamise ukusetshenziswa kanye ne-nitroblue tetrazolium (NBT) njenge-substrate yokuthola umsebenzi we-alkaline phosphatase.Uma i-BCIP i-dephosphorylated yi-alkaline phosphatase, amafomu emvula eluhlaza okwesibhakabhaka, okuvumela ukubonakala ngeso lengqondo ubukhona noma umsebenzi we-enzyme.
Le nhlanganisela iwusizo ikakhulukazi ezinhlelweni ezifana ne-immunohistochemistry, in situ hybridization, kanye ne-enzyme-linked immunosorbent assays (ELISAs) ukuze kutholwe ukuba khona noma ukwenziwa kwendawo kwama-biomolecules athile noma ama-nucleic acid.Imvula eluhlaza okwesibhakabhaka eyenziwe yi-BCIP inikeza isignali ebonakalayo esiza ekuhlonzeni nasekuhlaziyeni ama-molecule aqondiwe kumasampuli okuhlola.
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I-Sodium Salt Salt CAS: 139-41-3 Intengo Yomkhiqizi
I-N,N-Bis(2-hydroxyethyl) usawoti we-glycine sodium iyinhlanganisela yamakhemikhali esetshenziswa njenge-ejenti ephazamisayo emisebenzini ehlukahlukene ye-biochemical and biophysical.Isiza ukugcina izinga le-pH elizinzile ezimeni zokuhlola, iyenze ibe usizo ezifundweni zama-enzyme, ucwaningo lwamaprotheni, isiko lamaseli, nezindlela zokuvala zaseNtshonalanga.
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I-4-Aminophthalhydrazide AMPPD CAS:3682-14-2
I-4-Aminophthalhydrazide, eyaziwa nangokuthi i-4-APhH, iyinhlanganisela yamakhemikhali ene-molecular formula C8H8N2O.Ingokwesigaba se-hydrazide compounds futhi itholakala ku-phthalic acid.
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I-N-Acetyl-L-cysteine CAS: 616-91-1
I-N-Acetyl-L-cysteine (NAC) iwuhlobo oluguquliwe lwe-amino acid cysteine.Ihlinzeka ngomthombo we-cysteine futhi ingaguqulwa kalula ibe i-tripeptide glutathione, i-antioxidant enamandla emzimbeni.I-NAC yaziwa ngezakhiwo zayo ze-antioxidant kanye ne-mucolytic, okuyenza ibe wusizo ezinhlelweni ezahlukahlukene zezempilo.
Njenge-antioxidant, i-NAC isiza ukuvikela amaseli emonakalweni obangelwa ama-radicals mahhala, izinhlobo ze-oxygen esebenzayo, kanye nobuthi.Iphinde isekele i-glutathione synthesis, edlala indima ebalulekile ezinqubweni zokukhipha ubuthi emzimbeni kanye nokugcina amasosha omzimba anempilo.
I-NAC icwaningwe ngezinzuzo zayo ezingaba khona empilweni yokuphefumula, ikakhulukazi kubantu abanezimo ezifana ne-bronchitis engapheli, i-COPD, ne-cystic fibrosis.Ivamise ukusetshenziswa njenge-expectorant ukusiza amafinyila azacile futhi axege, okwenza kube lula ukusula imigudu yomoya.
Ngaphezu kwalokho, i-NAC ibonise isithembiso ekusekeleni impilo yesibindi ngokusiza ekukhipheni izinto ezinobuthi, njenge-acetaminophen, isidambisi sobuhlungu esivamile.Kungase futhi kube nemiphumela yokuzivikela ekulimaleni kwesibindi okubangelwa ukuphuza utshwala.
Ngaphezu kwezakhiwo zayo zokusekela i-antioxidant nokuphefumula, i-NAC iye yahlolwa ngezinzuzo zayo ezingaba khona empilweni yengqondo.Olunye ucwaningo lubonisa ukuthi kungase kube nomthelela omuhle ezinkingeni zemizwa, njengokucindezeleka kanye nesifo esicindezelayo (OCD).
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I-acetylthiocholine iodide CAS:1866-15-5
I-acetylthiocholine iodide iyinhlanganisela yamakhemikhali evame ukusetshenziswa njenge-substrate ekuhlolweni kwe-enzyme ukukala umsebenzi we-enzyme i-acetylcholinesterase (AChE).I-AChE iyi-enzyme eyenza i-hydrolyze i-neurotransmitter acetylcholine, isinyathelo esibalulekile sokunqamula ukudluliswa kwesignali phakathi kwamaseli ezinzwa.
Uma i-acetylthiocholine iodide isetshenziswa yi-AChE, iqembu le-acetyl liyasuswa, okuholela ekwakhekeni kwe-thiocholine ne-acetate ion.I-Thiocholine ibe isisabela ngento engenambala ebizwa ngokuthi i-DTNB (5,5′-dithiobis(2-nitrobenzoic acid)) ukuze kukhiqizwe inhlanganisela enombala ophuzi ebizwa ngokuthi 5-thio-2-nitrobenzoate, engalinganiswa nge-spectrophotometrically.Izinga lokuthuthukiswa kombala lilingana ngokuqondile nomsebenzi we-AChE kusampula.
