I-PIPES CAS: 5625-37-6 Intengo Yomkhiqizi

Amapayipi (i-piperazine-1,4-bisethanesulfonic acid) iyinhlanganisela yokubopha ye-zwitterionic esetshenziswa kakhulu ocwaningweni lwezinto eziphilayo kanye nezamakhemikhali. Inezici eziningana ezibalulekile kanye nezinhlelo zokusebenza, okuhlanganisa:

I-ejenti yokuvimba i-pH: I-PIPES iyi-buffer ephumelelayo esiza ukugcina uhla lwe-pH oluzinzile ekuhlolweni okuhlukahlukene kwezinto eziphilayo. Ivame ukusetshenziswa kwimidiya yokukhulisa amaseli, ukuhlolwa kwama-enzyme, kanye nezinhlelo zokusebenza zebhayoloji yama-molecular.

Umthamo ophezulu wokuvimba: Amapayipi anamandla amahle okuvimba ngaphakathi kobubanzi be-pH obungu-6.1 kuya ku-7.5, okwenza afaneleke ukugcina izimo ze-pH ezizinzile ezinhlelweni eziningi zezinto eziphilayo.

Ukusebenzisana okuncane nama-biomolecule: I-PIPES yaziwa ngokuphazamiseka kwayo okuphansi kwezinqubo ze-biochemical kanye nokubopha okuncane kumaphrotheni nama-enzyme, okwenza ibe yindawo ekahle yokugcina ubuqotho kanye nomsebenzi wama-biomolecule.

Ifanelekela ukuhlolwa okuncike ekushiseni: I-PIPES iyakwazi ukugcina izakhiwo zayo zokubopha ebangeni elibanzi lokushisa, kufaka phakathi amazinga okushisa aphakeme kanye namazinga okushisa aphezulu. Lokhu kuyenza ifanelekele ukuhlolwa okudinga ukuzinza nokunemba ezimweni zokushisa ezahlukahlukene.

Ukusetshenziswa kwe-Electrophoresis: Amapayipi avame ukusetshenziswa njenge-buffer kumasu e-gel electrophoresis, njenge-RNA noma i-DNA agarose gel electrophoresis, ngenxa yokumuncwa kwayo okuphansi kwe-UV kanye nezakhiwo zayo zokuhambisa umoya eziphezulu.

Ukwakheka kwemithi: I-PIPES isetshenziswa futhi ekwakhiweni kwemithi njenge-ejenti yokubopha, ihlinzeka ngokuqina nokugcina i-pH efanele ukuze imithi isebenze kahle.