3,3′,5,5′-Tetramethylbenzidine CAS:207738-08-7

I-3,3′, 5,5′-Tetramethylbenzidine, eyaziwa nangokuthi i-TMB, iyinhlanganisela yamakhemikhali evame ukusetshenziswa njenge-substrate ye-chromogenic ekuhlolweni kwe-enzyme-linked immunosorbent (ELISA) nakwezinye izivivinyo ze-biochemical. Ivame ukusetshenziswa ukuthola nokulinganisa ukuba khona kwama-enzyme afana ne-horseradish peroxidase (HRP) kumasampula ahlukahlukene e-biological. I-TMB idlula ekushintsheni kombala kusuka kokungenambala kuya koluhlaza okwesibhakabhaka lapho kukhona la ma-enzyme. Ngemva kwalokho, ukusabela kungamiswa ngokungeza i-asidi eguqula umbala oluhlaza okwesibhakabhaka ube umbala ophuzi wokugcina. Ukuqina kombala ophuzi kuhambisana nenani lama-enzyme akhona, okuvumela ukulinganiswa.

Oda noma Cela Isilinganiso

Imininingwane Yomkhiqizo

Amathegi Omkhiqizo

Ukusetshenziswa kanye Nomphumela

I-ELISA: I-TMB ivame ukusetshenziswa ekuhlolweni okusekelwe ku-ELISA ukuthola nokulinganisa ukuba khona kwamaprotheni, ama-antibodies, noma ama-antigen. Ukuqina kombala kuhambisana ngqo nokuhlushwa kwe-molecule eqondiwe.

Ukuhlolwa komsebenzi we-enzyme: I-TMB ingasetshenziswa ukukala umsebenzi wama-enzyme afana ne-HRP. Ngokukala izinga lokushintsha kombala, abacwaningi banganquma umsebenzi noma ukuhlushwa kwe-enzyme kusampula.

Ukufaka umbala kwezinto eziphilayo: I-TMB ingasetshenziswa ku-immunohistochemistry kanye ne-immunocytochemistry ukuze kubonwe izicubu ezithile noma izingxenye zeseli. Lapho i-TMB isabela ne-enzyme HRP, ikhiqiza ukujiya okunsundu noma okuluhlaza okwesibhakabhaka endaweni ye-molecule eqondiwe, okuvumela ukubonakala kwayo ngaphansi kwe-microscope.

Ukupakisha Umkhiqizo:

Ulwazi Olwengeziwe:

Ukwakheka	C16H23ClN2O
Ukuhlolwa	99%
Ukubukeka	Impuphu emhlophe
Inombolo ye-CAS	207738-08-7
Ukupakisha	Encane futhi enkulu
Impilo Yeshelufu	iminyaka engu-2
Isitoreji	Gcina endaweni epholile neyomile
Isitifiketi	I-ISO.