5-Bromo-4-chloro-3-indolyl phosphate disodium salt CAS:102185-33-1

I-5-Bromo-4-chloro-3-indolyl phosphate disodium salt (BCIP) iyinhlanganisela yamakhemikhali evame ukusetshenziswa ku-biology yamangqamuzana kanye nezicelo ze-biochemistry. Iyisithako se-chromogenic sama-enzyme e-alkaline phosphatase.

I-BCIP ivame ukusetshenziswa kanye ne-nitroblue tetrazolium (NBT) njenge-substrate ukuthola umsebenzi we-alkaline phosphatase. Uma i-BCIP ikhishwa yi-alkaline phosphatase, kwakheka i-blue precipitate, okuvumela ukubonakala kokuba khona noma umsebenzi we-enzyme.

Le nhlanganisela iwusizo kakhulu ekusetshenzisweni okufana ne-immunohistochemistry, i-in situ hybridization, kanye ne-enzyme-linked immunosorbent assays (ELISAs) ukuthola ukuba khona noma indawo yama-biomolecule athile noma ama-nucleic acid. I-precipitate eluhlaza okwesibhakabhaka eyenziwe yi-BCIP inikeza isignali ebonakalayo esiza ekuhlonzeni nasekuhlaziyeni ama-molecule aqondiwe kumasampula okuhlola.

Oda noma Cela Isilinganiso

Imininingwane Yomkhiqizo

Amathegi Omkhiqizo

Ukusetshenziswa kanye Nomphumela

I-Immunohistochemistry (IHC): I-BCIP ivame ukusetshenziswa ekuhlolweni kwe-IHC ukuze kubonwe ubukhona noma indawo yama-antigen athile kumasampula ezicubu. Ukukhanya okuluhlaza okwesibhakabhaka okukhiqizwa yi-BCIP kuvumela ukuhlonza izindawo ze-antigenic futhi kungasetshenziswa ekuhlaziyweni kwekhwalithi noma kwesilinganiso esincane.

I-In situ hybridization (ISH): I-BCIP ivame ukusetshenziswa kumasu e-ISH ukuthola nokuthola izindawo ezithile ze-nucleic acid sequences, njenge-mRNA noma i-DNA. I-blue precipitate eyakhiwe yi-BCIP ikhombisa ukuba khona kwe-target sequences futhi isiza ekubonisweni kokusatshalaliswa kwazo ngaphakathi kwamaseli noma izicubu.

Ukuhlolwa kwe-immunosorbent okuxhunywe ku-enzyme (ama-ELISA): I-BCIP ngezinye izikhathi isetshenziswa kuma-ELISA ukuthola ukuba khona noma ukuhlushwa kwama-biomolecule athile, njengeprotheni noma ama-antibodies. Ngokuhlanganisa i-BCIP nelebula le-enzyme elifanele nokusebenzisa i-alkaline phosphatase ukuvuselela ukusabela, umbala oluhlaza okwesibhakabhaka okhiqizwayo ungabonisa ukuba khona kanye nobuningi be-biomolecule ethakazelisayo.

Ukupakisha Umkhiqizo:

Ulwazi Olwengeziwe:

Ukwakheka	C8H7BrClNNaO4P
Ukuhlolwa	99%
Ukubukeka	Impuphu emhlophe
Inombolo ye-CAS	102185-33-1
Ukupakisha	Encane futhi enkulu
Impilo Yeshelufu	iminyaka engu-2
Isitoreji	Gcina endaweni epholile neyomile
Isitifiketi	I-ISO.