I-Diethylene triamine pentaacetic acid CAS:67-43-6

I-Diethylene triamine pentaacetic acid (DTPA) iyinhlanganisela yamakhemikhali evame ukusetshenziswa njenge-chelating agent. Inamandla okubopha ngama-ion ensimbi esindayo, ikakhulukazi lawo afana ne-lead, i-mercury, ne-cadmium, ukuze yakhe ama-complexes azinzile. I-DTPA isetshenziswa ezinhlotsheni ezahlukene zokusebenza, okuhlanganisa ukulungiswa kwemvelo, ukwelashwa kwezokwelapha, izinqubo zezimboni, kanye nemithi ye-radiopharmaceuticals. Izici zayo ze-metal-chelating ziyenza isebenze kahle ekususeni izinsimbi ezisindayo emanzini angcolile, ekwelapheni ubuthi bensimbi esindayo, ekuvimbeleni ukuphazamiseka kokusabela kwamakhemikhali, kanye nokwenza kube lula ukulethwa kwemithi yokwelapha okuqondiwe. Sekukonke, i-DTPA iyinhlanganisela eguquguqukayo enezinhlelo zokusebenza ezibalulekile ezimbonini ezahlukene.

Oda noma Cela Isilinganiso

Imininingwane Yomkhiqizo

Amathegi Omkhiqizo

Ukusetshenziswa kanye Nomphumela

Ukungasebenzi kwama-RNases: Inhloso eyinhloko ye-DEPC ukuvimbela ama-RNases, angama-enzyme angalimaza i-RNA. I-DEPC isabela nama-histidine residues kuma-RNases, iguqula izindawo zawo ezisebenzayo futhi izenze zingasebenzi. Lokhu kuqinisekisa ukugcinwa kobuqotho be-RNA ngesikhathi sokuhlolwa okufana nokuhlukaniswa kwe-RNA, ukubhalwa kabusha, kanye nokulandelana kwe-RNA.

Ukulondolozwa kwamasampula e-RNA: Ukwelashwa kwe-DEPC kuvame ukusetshenziswa emishinini yelabhorethri, njengamashubhu e-microcentrifuge, amathiphu e-pipette, kanye namanzi asetshenziswa ekuhlolweni kwe-RNA. Lokhu kusiza ukuvimbela noma yimaphi ama-RNase asele angaba khona futhi kuvimbele ukuwohloka kwe-RNA ngesikhathi sokuphathwa nokugcinwa kwesampula.

Ukulungiswa kwe-reagent engena-RNAse: I-DEPC ingasetshenziswa ukwelapha izixazululo noma ama-reagent okudingeka angabi nama-RNAse, njenge-buffers, izixazululo ze-Tris-EDTA (TE), noma amanzi asetshenziswa ekuhlolweni kwe-molecular biology. Lezi zixazululo eziphathwe zingasetshenziswa ngokuphephile ukunciphisa ukuwohloka kwe-RNA.

Ukuguqulwa kwe-DNA: I-DEPC ingasabela namaqembu ama-amino kuma-molecule e-DNA, okuholela ekuguqulweni kwezisekelo ze-DNA ezihlanganiswe yi-carbethoxylated. Lokhu kungasetshenziswa ezicelweni ezithile, njengokusabela kokulandelana kwe-DNA noma ukuguqula i-DNA ukuze kufundwe ngokuxhumanisa.

Ukupakisha Umkhiqizo:

Ulwazi Olwengeziwe:

Ukwakheka	C6H10O5
Ukuhlolwa	99%
Ukubukeka	Uketshezi olungenambala
Inombolo ye-CAS	1609-47-8
Ukupakisha	Encane futhi enkulu
Impilo Yeshelufu	iminyaka engu-2
Isitoreji	Gcina endaweni epholile neyomile
Isitifiketi	I-ISO.