I-DEPC CAS:1609-47-8 Intengo Yomkhiqizi

I-Diethyl pyrocarbonate (DEPC) iyinhlanganisela yamakhemikhali evame ukusetshenziswa emalabhorethri ebhayoloji yama-molecular. Iwuketshezi olucacile nolungenambala olusabela kakhulu kuma-compound e-nucleophilic njenge-amines kanye ne-thiols. I-DEPC isetshenziswa kakhulu ukuvimba ama-RNases (ribonucleases), ama-enzyme angalimaza i-RNA, ngokushintsha izindawo zawo ezisebenzayo. Ifinyelela lokhu ngokusabela ngama-residues e-histidine ku-enzyme, abalulekile emsebenzini wayo. Lokhu kwenza i-DEPC ibe ithuluzi elibalulekile ekuhlolweni okuhlobene ne-RNA futhi kuqinisekisa ukugcinwa kobuqotho be-RNA. Ngaphezu kwezakhiwo zayo zokuvimbela i-RNase, i-DEPC ingaguqula namanye ama-biomolecule, njengeprotheni kanye ne-DNA, ngenxa yokusabela kwayo namaqembu e-nucleophilic. Kufanele kuqashelwe lapho kuphathwa i-DEPC, njengoba inobuthi futhi ingase ibe yi-mutagenic.

Oda noma Cela Isilinganiso

Imininingwane Yomkhiqizo

Amathegi Omkhiqizo

Ukusetshenziswa kanye Nomphumela

Ukungasebenzi kwama-RNases: Inhloso eyinhloko ye-DEPC ukuvimbela ama-RNases, angama-enzyme angalimaza i-RNA. I-DEPC isabela nama-histidine residues kuma-RNases, iguqula izindawo zawo ezisebenzayo futhi izenze zingasebenzi. Lokhu kuqinisekisa ukugcinwa kobuqotho be-RNA ngesikhathi sokuhlolwa okufana nokuhlukaniswa kwe-RNA, ukubhalwa kabusha, kanye nokulandelana kwe-RNA.

Ukulondolozwa kwamasampula e-RNA: Ukwelashwa kwe-DEPC kuvame ukusetshenziswa emishinini yelabhorethri, njengamashubhu e-microcentrifuge, amathiphu e-pipette, kanye namanzi asetshenziswa ekuhlolweni kwe-RNA. Lokhu kusiza ukuvimbela noma yimaphi ama-RNase asele angaba khona futhi kuvimbele ukuwohloka kwe-RNA ngesikhathi sokuphathwa nokugcinwa kwesampula.

Ukulungiswa kwe-reagent engena-RNAse: I-DEPC ingasetshenziswa ukwelapha izixazululo noma ama-reagent okudingeka angabi nama-RNAse, njenge-buffers, izixazululo ze-Tris-EDTA (TE), noma amanzi asetshenziswa ekuhlolweni kwe-molecular biology. Lezi zixazululo eziphathwe zingasetshenziswa ngokuphephile ukunciphisa ukuwohloka kwe-RNA.

Ukuguqulwa kwe-DNA: I-DEPC ingasabela namaqembu ama-amino kuma-molecule e-DNA, okuholela ekuguqulweni kwezisekelo ze-DNA ezihlanganiswe yi-carbethoxylated. Lokhu kungasetshenziswa ezicelweni ezithile, njengokusabela kokulandelana kwe-DNA noma ukuguqula i-DNA ukuze kufundwe ngokuxhumanisa.

Ukupakisha Umkhiqizo:

Ulwazi Olwengeziwe:

Ukwakheka	C6H10O5
Ukuhlolwa	99%
Ukubukeka	Uketshezi olungenambala
Inombolo ye-CAS	1609-47-8
Ukupakisha	Encane futhi enkulu
Impilo Yeshelufu	iminyaka engu-2
Isitoreji	Gcina endaweni epholile neyomile
Isitifiketi	I-ISO.