I-Ethidium Bromide CAS:1239-45-8

I-Ethidium bromide (EtBr) iwudayi ojwayelekile we-fluorescent osetshenziswa ku-molecular biology kanye ne-biochemistry ukuze kubonwe ama-nucleic acid, ikakhulukazi i-DNA, ku-agarose gel electrophoresis. Ine-affinity ephezulu ye-DNA futhi, uma ivezwa ekukhanyeni kwe-ultraviolet (UV), iyakhanya futhi ikhiphe umbala obomvu-orenji. Lokhu kuvumela abacwaningi ukuthi babone futhi bahlaziye kalula izingcezu ze-DNA ezihlukaniswe ngobukhulu ku-gel matrix.

I-EtBr ihlangana phakathi kwezibili eziyisisekelo ze-DNA ne-RNA, okubangela ushintsho ezimpawini zayo ze-fluorescence. Ivame ukungezwa kuma-gel e-agarose noma ixutshwe namasampula e-DNA ngaphambi kwe-electrophoresis. Lapho ijeli ivezwa ekukhanyeni kwe-UV, ama-band e-DNA aqukethe i-EtBr abonakala njengama-band anombala o-orenji akhanyayo ngokumelene nesizinda esimnyama, okwenza kube lula ukubona ngeso lengqondo nokuhlaziya izingcezu ze-DNA.

Oda noma Cela Isilinganiso

Imininingwane Yomkhiqizo

Amathegi Omkhiqizo

Ukusetshenziswa kanye Nomphumela

Ukubona nge-DNA/RNA: I-EtBr ivame ukusetshenziswa ukubona ngeso lengqondo i-DNA ne-RNA ku-agarose gel electrophoresis. Ihlangana phakathi kwezibili eziyisisekelo zama-molecule e-nucleic acid, okubangela ukuba akhanye lapho evezwa ekukhanyeni kwe-UV. Lokhu kuvumela abacwaningi ukubona ngeso lengqondo futhi bahlaziye ukuhlukaniswa kwezingcezu ze-DNA noma ze-RNA ngokusekelwe kusayizi noma ukulandelana kwazo.

Ukupenda i-DNA: I-EtBr ingasetshenziswa ukupenda i-DNA ezinqubweni ezahlukene zokuhlola. Ingangezwa kumasampula e-DNA, njenge-plasmids ehlanziwe noma imikhiqizo ye-PCR, ukuqinisekisa ukuba khona kwawo kanye nokuhlushwa kwawo. I-DNA enemibala ingabe isibonakala kusetshenziswa ukukhanya kwe-UV noma uhlelo lokuthwebula izithombe lwejeli.

Ukulinganiswa kwe-DNA: I-EtBr ingasetshenziswa ekulinganiseni ukuhlushwa kwe-DNA. Ngokuqhathanisa ubukhali bama-band e-DNA anemibala ye-EtBr kujeli ye-agarose namazinga aziwayo okuhlushwa kwe-DNA, inani elilinganiselwe le-DNA kusampula lingatholakala.

I-Fluorescent Microscopy: I-EtBr ingasetshenziswa ku-fluorescence microscopy ukuze ifake umbala futhi ibone ngeso lengqondo i-DNA emaseli noma ezicutshini eziphilayo. Ivumela abacwaningi ukuthi batadishe indawo ye-DNA kanye nokuguquguquka kwayo ngaphakathi kwamaseli noma babone ukuhlukana kwe-DNA kumaseli e-apoptosis.

Ukupakisha Umkhiqizo:

Ulwazi Olwengeziwe:

Ukwakheka	C21H20BrN3
Ukuhlolwa	99%
Ukubukeka	Impuphu ebomvu kuya kophuzi omnyama
Inombolo ye-CAS	1239-45-8
Ukupakisha	Encane futhi enkulu
Impilo Yeshelufu	iminyaka engu-2
Isitoreji	Gcina endaweni epholile neyomile
Isitifiketi	I-ISO.