4-NITROPHENYL-ALPHA-D-MANNOPYRANOSIDE CAS:10357-27-4

Ama-Enzyme substrates: I-4NPM ingasetshenziswa njenge-substrate yama-enzyme ahlukahlukene, kuhlanganise nama-glycosidase nama-enzyme ahlobene.Lawa ma-enzyme ahlukanisa isibopho se-glycosidic phakathi kwe-mannose ne-4NPM, okuholela ekukhululweni kwe-nitrophenyl moiety.Izinga le-substrate hydrolysis lingalinganiswa nge-spectrophotometrically ngokuqapha ukumunca kweqembu le-nitrophenyl elikhishiwe ngobude obuthile begagasi.Lokhu kuvumela abacwaningi ukuthi bahlole umsebenzi we-enzyme kanye ne-kinetics.

Ukuhlolwa kwe-carbohydrate metabolism: Ngokusebenzisa i-4NPM njenge-substrate, abacwaningi bangakwazi ukutadisha umsebenzi wama-enzyme abandakanyeka ku-carbohydrate metabolism, njenge-alpha-mannosidases.Lawa ma-enzyme enza i-hydrolyze izibopho ze-glycosidic kumakhompiyutha aqukethe i-mannose, futhi umsebenzi wawo ungalinganiswa ngokuqapha ukukhululwa kwe-nitrophenyl moiety kusuka ku-4NPM.

Izifundo ze-Glycosylation: I-4NPM ingasetshenziswa futhi ekuhlolweni ukuze kuphenywe ama-enzyme abandakanyeka kuzinqubo ze-glycosylation.I-Glycosylation iyinqubo yokunamathisela ama-molecule kashukela kumaprotheni noma kwamanye ama-molecule, futhi ama-enzyme amaningi ahilelekile kule nqubo.Ngokusebenzisa i-4NPM njenge-substrate eyamukelayo, abacwaningi bangakala ukudluliswa kwe-sugar moiety ku-4NPM ngama-enzyme athile ahilelekile ekuphenduleni kwe-glycosylation.

Ukuhlola ama-enzyme inhibitors noma ama-activators: I-4NPM ingasetshenziswa ekuhlolweni kokuhlolwa okuphezulu ukuze kuhlonzwe izinhlanganisela ezivimbela noma ezivula ama-enzyme athile.Ngokuhlola umphumela wezinhlanganisela zokuhlola ku-hydrolysis noma ukuguqulwa kwe-4NPM ngama-enzyme okuhlosiwe, abacwaningi bangakwazi ukuhlonza ama-ejenti okwelapha angaba khona noma ama-probe amakhemikhali awusizo okutadisha umsebenzi we-enzyme.