5-Bromo-4-chloro-3-indolyl-N-acetyl-beta-D-glucosaminide CAS:4264-82-8

I-5-Bromo-4-chloro-3-indolyl-N-acetyl-beta-D-glucosaminide (X-Gluc) iyi-substrate ye-chromogenic evame ukusetshenziswa ukuthola umsebenzi we-beta-glucuronidase (GUS). I-GUS iyi-enzyme etholakala ezintweni eziphilayo ezahlukahlukene, kufaka phakathi amabhaktheriya, izitshalo, nezilwane ezincelisayo. I-X-Gluc ivame ukusetshenziswa ekuhlolweni kwentatheli ye-GUS kanye nocwaningo lwebhayoloji yama-molecule.

Ukusetshenziswa okuyinhloko kwe-X-Gluc kusezindleleni zokufaka imibala ze-histochemical, lapho ingabona khona ukuvezwa kanye nomsebenzi we-enzyme ye-GUS. Le substrate ingena kumaseli futhi i-hydrolyzed yi-GUS, okuholela ekwakhekeni kwe-blue precipitate noma umkhiqizo ongancibiliki. Lokhu kufaka imibala eluhlaza okwesibhakabhaka kuvumela abacwaningi ukuthi babone futhi baqonde umsebenzi we-GUS kumaseli, izicubu, kanye nezinto eziphilayo eziphelele.

I-X-Gluc ingasetshenziswa futhi ekuhlolweni kwenani ukuze kulinganiswe umsebenzi we-enzyme ye-GUS. Ukuqina kombala oluhlaza okwesibhakabhaka noma inani lomkhiqizo owenziwe kungahlotshaniswa nezinga lokuvezwa kwe-GUS noma umsebenzi wayo we-enzyme.

Ngaphezu kwalokho, i-X-Gluc isetshenziswe ocwaningweni lwezakhi zofuzo zezitshalo ukuze ifunde ukubonakaliswa kwezakhi zofuzo, umsebenzi wokukhuthaza, kanye nokuguqulwa kwezitshalo. Iphinde yasetshenziswa ezinhlelweni zamagciwane ukuze kuhlanganiswe futhi kutholakale amaprotheni e-GUS fusion.