4-Nitrophenyl-alpha-D-galactopyranoside CAS:7493-95-0

I-substrate yokuhlolwa kwe-enzymatic: I-4-Nitrophenyl-alpha-D-glucopyranoside ivame ukusetshenziswa njenge-substrate ekuhlolweni kwe-enzymatic ukukala umsebenzi we-glycosidase namanye ama-enzyme ahilelekile ku-carbohydrate metabolism.Lawa ma-enzyme angahlukanisa isibopho phakathi kwe-glucose neqembu le-4-nitrophenyl, akhiphe umkhiqizo ophuzi obizwa ngokuthi i-4-nitrophenol.Inani le-4-nitrophenol elakhiwe lilingana ngokuqondile nomsebenzi we-enzyme, okuvumela ukulinganiswa komthamo womsebenzi we-enzyme.

Ukutholwa komsebenzi we-enzyme: Ngokusebenzisa i-4-Nitrophenyl-alpha-D-glucopyranoside njenge-substrate, umsebenzi wama-enzyme athile ungalinganiswa.Isibonelo, ama-enzyme e-glycosidase afana ne-beta-glucosidase, i-alpha-glucosidase, noma i-beta-galactosidase angakwazi ukuhlukanisa inhlanganisela, akhiphe i-4-nitrophenol, engatholwa nge-photometric.Le ndlela isetshenziswa kakhulu ezinhlelweni ezahlukahlukene zezokwelapha, ezemithi, kanye ne-biotechnological.

Ukuhlolwa komphumela ophezulu: Ngenxa yokuhambisana kwayo nezinhlelo zokuhlonza ezizenzakalelayo, i-4-Nitrophenyl-alpha-D-glucopyranoside ivame ukusetshenziswa ekuhlolweni kokuhlolwa komphumela ophezulu.Le nhlanganisela ivumela ukubonakaliswa okusheshayo nokusebenza kahle komsebenzi we-enzyme, okwenza kube usizo ekutholeni izidakamizwa, ubunjiniyela be-enzyme, kanye nezifundo zokuvimbela ama-enzyme.

Izicelo zokuxilonga: I-4-Nitrophenyl-alpha-D-glucopyranoside ingasetshenziswa ezinhlelweni zokuxilonga ukuze kutholwe ubukhona noma umsebenzi wama-enzyme athile ahlobene nezifo ezahlukahlukene.Isibonelo, umsebenzi wama-enzyme athile ungasetshenziswa njengophawu lokuxilonga izifo ezithile, futhi i-4-Nitrophenyl-alpha-D-glucopyranoside ingasetshenziswa njenge-substrate ukukala umsebenzi walawa ma-enzyme kumasampula omtholampilo.