I-FLUORESCEIN MONO-BETA-D- GALACTOPYRANOSIDE CAS:102286-67-9

I-Fluorescein mono-beta-D-galactopyranoside, eyaziwa nangokuthi i-FMG, iyinhlanganisela ekhanyayo evame ukusetshenziswa njenge-substrate ezivivinyweni ezahlukahlukene ze-biochemical kanye ne-cell biology. Itholakala ku-methyl-beta-D-galactopyranoside ngokuyihlanganisa ne-molecule ye-fluorescein. I-FMG isetshenziswa kabanzi ukutadisha umsebenzi we-beta-galactosidase, i-enzyme evuselela i-hydrolysis ye-lactose ibe yi-galactose kanye ne-glucose. Ngokusebenzisa i-FMG njenge-substrate, abacwaningi bangaqapha umsebenzi we-enzyme we-beta-galactosidase ngokulinganisa ukuphuma kwe-fluorescence. I-hydrolysis ye-FMG yi-beta-galactosidase iholela ekukhishweni kwe-fluorescein, okuholela ekwandeni kwesignali ye-fluorescent engalinganiswa. Le nhlanganisela isetshenziswa futhi ukuphenya ukuqashelwa kwama-carbohydrate kanye nokusebenzisana. I-FMG ingasetshenziswa njenge-probe yama-molecule ukutadisha ukubopha kwama-lectin (amaprotheni abopha ngqo kuma-carbohydrate) kuma-carbohydrate aqukethe i-galactose. Ukubopha kwama-FMG-lectin complexes kungatholakala futhi kulinganiswe ngokusekelwe ezinguqukweni zokukhishwa kwe-fluorescence. Sekukonke, i-FMG iyithuluzi eliguquguqukayo lokufunda umsebenzi we-enzyme kanye nokuqashelwa kwama-carbohydrate, okunikeza indlela elula nebucayi yokukala i-fluorescence nokuhlola lezi zinqubo zebhayoloji.

Oda noma Cela Isilinganiso

Imininingwane Yomkhiqizo

Amathegi Omkhiqizo

Ukusetshenziswa kanye Nomphumela

I-Fluorescein mono-beta-D-galactopyranoside (FMG) iyi-molecule evame ukusetshenziswa ocwaningweni lwezinto eziphilayo njenge-substrate ukuthola ubukhona kanye nomsebenzi we-enzyme ye-beta-galactosidase. I-FMG iyi-derivative ye-sugar lactose futhi ihlanganiswe ne-molecule ye-fluorescein.

Umphumela oyinhloko we-FMG ukuthi i-hydrolyzed ngokukhethekile yi-beta-galactosidase, i-enzyme ephula i-lactose ibe yi-galactose kanye ne-glucose. Lokhu hydrolysis ye-enzyme ye-FMG kuholela ekukhishweni kwe-fluorescein, ekhipha isignali enamandla ye-fluorescence.

Ukusetshenziswa okuyinhloko kwe-FMG ukuthola nokulinganisa umsebenzi we-beta-galactosidase kumasampula ahlukahlukene. Le enzyme itholakala ezintweni eziningi eziphilayo, kufaka phakathi amabhaktheriya namaseli ezilwane ezincelisayo, futhi umsebenzi wayo ungabonisa izinqubo ezahlukahlukene zamaseli kanye nezindlela ze-metabolic.

Ngokusebenzisa i-FMG njenge-substrate, umsebenzi we-beta-galactosidase ungalinganiswa ngokuqapha i-fluorescence ekhishwa yi-fluorescein ekhululiwe. Lokhu kulinganisa kungenziwa ngezindlela ezahlukahlukene zokuhlola, kufaka phakathi ukuhlolwa kwe-in vitro kanye nezifundo ze-live cell imaging.

Ngaphezu kwalokho, i-FMG ingasetshenziswa njengethuluzi lokufunda ukusatshalaliswa kanye nendawo ye-beta-galactosidase ngaphakathi kwamaseli. Ngokusebenzisa amasu e-fluorescent microscopy, abacwaningi bangabona ngeso lengqondo i-fluorescence ekhishwa yi-FMG ngesikhathi se-hydrolysis, okubavumela ukuthi balandelele umsebenzi wendawo kanye nesikhathi se-beta-galactosidase.

Isampula Yomkhiqizo

Ukupakisha Umkhiqizo:

Ulwazi Olwengeziwe:

Ukwakheka	C26H22O10
Ukuhlolwa	99%
Ukubukeka	Impuphu emhlophe
Inombolo ye-CAS	102286-67-9
Ukupakisha	Encane futhi enkulu
Impilo Yeshelufu	iminyaka engu-2
Isitoreji	Gcina endaweni epholile neyomile
Isitifiketi	I-ISO.